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Volume 16, Issue 9 (12-2013)                   J Arak Uni Med Sci 2013, 16(9): 101-110 | Back to browse issues page

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Nowroozi J, Siasi Torbati E, Baharvand R. Isolation of Listeria Monocytogenes from Meat Products and Study on PrfA Gene in Bacteria Isolated and Compaire Them with Clinical Samples and Standard Samples . J Arak Uni Med Sci. 2013; 16 (9) :101-110
URL: http://jams.arakmu.ac.ir/article-1-2420-en.html
1- 1. Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
2- 1. Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran , baharkia44@yahoo.com
Abstract:   (6722 Views)

Background: Listeria is a gram positive bacterium, facultive intracellular pathogene. Prf A gene has an important role in virulence. The purpose of this study was to determine of the presence of Listeria monocytogenes in various meat products and presence of prf A gene in bacteria isolated from food samples and compare them with clinical samples and standard samples.

Materials and Methods: During 6 months of 60 samples of beef, chicken, ham, cocktail, sausage, from a butchery’s Khoramabad and shops in Khoramabad and Tehran were collected. L. monocytogenes was isolated according to cold enrichment method and prf A gene were analyzed by PCR (polymerase chain reaction) method. Statistical analysis was performed with the software SPSS (statistical package for social science).

Results: From 60 samples of meat and meat products, 8 (13.3%) were positive for Listeria spp. Among in these samples, 4 cases of L. monocytogenes (6.6%), 3 cases (5%), L. innocua and 1 case (1.6%) L. welshimri, were isolated which L. innocua was isolated from meat and poultry samples and L. monocytogens from meat, chicken, ham and L. welshimeri from meat were isolated. Prf A gene was detected in isolated L. monocytogenes and donated bacteria from dairy products, clinical and standard samples, 2 cases of donated samples of vegetable contained prf A genes.

Conclusion: The prf A gene (activator of transcription and regulators the expression of other virulence genes), in the studied samples were observed. This gene plays a role in pathogenesis. Because these bacteria are transmitted through food and is a serious threat to public health. Thus identification of bacteria especially its genes could be possible to find some ways to prevent the bacteria and avoid disease from it.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2013/07/17 | Accepted: 2013/11/20

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