Background: Gene therapy is a recent promising treatment that effective gene transfer is considered as its most important step. Furthermore noninvasive method of transfer will be important to, when gene therapy is supposed to be applied. Administration of drugs in oral rout is more appreciated by patients. Loading and release rates are very important in targeting and effectiveness of transfer in all different methods that have been used for oral drug transfer. Here, we have studied packing of gene particles into two different enteric coats and compared these two coats in loading of entrapped materials and there release rate in vitro.

Materials and Methods: First, DNA was mixed with chitosan by coacervation technique and resulted polyplexes were coated using solvent evaporation technique. FTIR and two different pHs, less and more but near eudragit pKa, were used to evaluate formation of particles and their behavior.

Results: Formed particles have similar stability in low pH and their differences are trivial. Eudragit L100 release rate is really slower than L100-55 and gradual. Eudragit L100 shows better ability in loading rate.

Conclusion: According to two formed particles' behavior, eudragit L100-55 might be used in oral gene transfer targeting of initial part of small intestine and eudragit L100 might be used for wider surface of small intestine, from the initial to the end part, and colon.