Volume 19, Issue 8 (11-2016)                   J Arak Uni Med Sci 2016, 19(8): 16-25 | Back to browse issues page

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Barari M, Sari S, Ebrahemi A. Screening of Common Chromosomal Disorders in Iranian Women with Hydatidiform Mole using QF-PCR. J Arak Uni Med Sci 2016; 19 (8) :16-25
URL: http://jams.arakmu.ac.ir/article-1-4477-en.html
1- Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2- PhD in Molecular and Cellular Biology, Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran , sari.s@iaups.ac.ir
3- PhD in Genetics, Research Institute for Endocrine Sciences Metabolism of SMBU, Tehran, Iran
Abstract:   (3606 Views)

Abstract

Background:  Hydatidiform Mole is a benign trophoblastic tumor is made of ectopic pregnancy. Abnormalities in the number or structure of chromosomes are causes of Hydatidiform Mole common numerical disorders resulted from proliferating repetitive sequences markers as called STR were studied in the region of chromosome X, Y, 13, 18 and 21. This study aimed to investigate chromosomal disorders prevalent in women with hydatidiform mole that was performed using QF-PCR techniques.

Materials and Methods: In this study, 50 women with hydatidiform mole and 80 healthy women as controls were selected. For studying the chromosomal abnormalities resulted of proliferating STR, Chromo Quant QF-PCR kit was used. Polymerase chain reaction was performed in PCR machine. Then electrophoresis was performed on Genetic Analyzer. Finally, amplified fragment were analyzed by Gene Marker software Statistical analysis was performed using SPSS version 19, and t-test. Data were expressed as mean ± SD. In this test, p <0.05 represents significant level between two groups.

Results:  In this study ،of 50 samples, 8 samples of 47XXY (16%), 40 samples of trisomy 21 (80%) and 2 cases of trisomy 18 (4%) were identified.

Conclusion: Anomalies Trisomy 21 (41 ± 1.58) and 47XXY (9.62 ± 1.36) are significantly associated with mydatidiform mole disease (p <0.001). The highest percentage of samples with trisomy 21 and 47XXY had the disease. So, it indicates that these anomalies have the highest percentage in the disease.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2016/06/11 | Accepted: 2016/08/13

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