Background: In the recent years, introducing plant materials with the ability to destroy or control of biofilm formation of Streptococcus mutans is highly regarded.This study was planned to investigate the anti-bacterial activity and biofilm inhibition of Satureja Khuzestanica Jamzad against biofilm and single form of S.mutans.

Materials and Methods: The plant material collected, air dried and powdred. Then thier methanolic extract prepared by maceration method.The concentrated extract then diluted by sterile phosphate buffer.The anti-bacterial and biofilm inhibition activity of the extracts evaluated by dick diffiusion and micro titeration method respectively. Also, bioinformatic study of glucansucrase inhibition by dominant plant compounds of S.Khuzestanica Jamzad investigated by molecular docking method using Auto dock4 software.

Results: The results revalued that all tested extracts especially leaf ectract had significant antibacterial activity against single form of S.mutans.Results also showed that leaf and stem extract had most and least biofilm inhibition with 70 and 13 presend inhibition, respectively. The bioinformatics results also confirmed that studied phytochemicals especially gama-terpinen, carvacrol and beta-bisabolene can effectively inhibit the glucansucrase.

Conclusion: The results of present study showed that S.Khuzestanica Jamzad had significant antibacterial activity against single form of S.mutans and gama-terpinen, carvacrol and beta-bisabolene are most effective compounds in biofilm inhibition.