Volume 21, Issue 7 (2-2019)
J Arak Uni Med Sci 2019, 21(7): 28-38 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khalili B S, Hamedi J, Haghighat S. Isolation and Identification Rare Actinobacteria from Persian Gulf and Oman Sea. J Arak Uni Med Sci 2019; 21 (7) :28-38
URL: http://jams.arakmu.ac.ir/article-1-5109-en.html
URL: http://jams.arakmu.ac.ir/article-1-5109-en.html
1- Department of Microbiology, Faculty of Advanced Sciences and Technologies, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
2- Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran. Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran. , jhamedi@ut.ac.ir
2- Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran. Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran. , jhamedi@ut.ac.ir
Abstract: (2512 Views)
| Background and Aim: The widespread use of antibiotics has been led to increased emergence of antibiotic resistant bacteria and high mortality and morbidity rates due to infectious diseases. Pseudomonas aeruginosa is one of the most important causes of nosocomial infections, which shows high resistance to a wide range of antibiotics. So, finding new and effective antimicrobial compounds in order to overcome antibiotic resistant infectious diseases is so critical. Screening of native actinobacteria can be an effective strategy to find novel antimicrobial compounds. The aim of current study was isolation, screening and identification of rare actinobacteria to find the strains which produce antimicrobial compounds against P. aeruginosa. Material and Methods: Thirty samples of water and sediments were collected from Persian Gulf and Oman Sea and used for isolation of actinobacterial strains. After isolation of actinobacteria, their metabolites were extracted and their anti-P. aeruginosa activities were investigated. Minimum inhibitory concentration (MIC) of the most efficient extract was determined using broth microdilution method. Finally, the most efficient strain was identified. Ethical Considerations: In this study, all principles of biosafety and bioethics have been considered. Findngs: Fifty actinobacteria were isolated from water and sediments. Five isolates had considerable antimicrobial activity. MIC value of the most efficient extract against P. aeruginosa was 100 µg/ml. Molecular analysis of 16SrRNA showed that the most effective fermentation broth extract belongs to Micromonospora and has 99.8% similarity to M. chalcea. Conclusion: The current study revealed that the water of southern Iran and their sediments are promising sources of potent rare Actinobacteria in the production of antimicrobial compounds against P. aeruginosa. |
References
1. Okeke IN. Poverty and root causes of resistance in developing countries. InAntimicrobial resistance in developing countries Springer, New York, NY. 2010. 27-35.
2. Parkins MD, Gregson DB, Pitout JD, Ross T, Laupland KB. Population-based study of the epidemiology and the risk factors for Pseudomonas aeruginosa bloodstream infection. Infection. 2010; 38(1):25-32.
3. Johnson LE, D'agata EM, Paterson DL, Clarke L, Qureshi ZA, Potoski BA, Peleg AY. Pseudomonas aeruginosa bacteremia over a 10‐year period: multidrug resistance and outcomes in transplant recipients. Transpl Infect Dis. 2009; 11(3):227-34.
4. Wiegand I, Marr AK, Breidenstein EB, Schurek KN, Taylor P, Hancock RE. Mutator genes giving rise to decreased antibiotic susceptibility in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 2008; 52(10):3810-3.
5. Baumgart AM, Molinari MA, Silveira AC. Prevalence of carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii in high complexity hospital. Braz J Infect Dis. 2010; 14(5):433-6.
6. Tuon FF, Gortz LW, Rocha JL. Risk factors for pan-resistant Pseudomonas aeruginosa bacteremia and the adequacy of antibiotic therapy. Braz J Infect Dis. 2012; 16(4):351-6.
7. Khan ST, Takagi M, Shin-ya K. Actinobacteria associated with the marine sponges Cinachyra sp., Petrosia sp., and Ulosa sp. and their culturability. Microbes Environ. 2012; 27(1):99-104.
8. Wilke MS, Heller M, Creagh AL, Haynes CA, McIntosh LP, Poole K, Strynadka NC. The crystal structure of MexR from Pseudomonas aeruginosa in complex with its antirepressor ArmR. Proc Natl Acad Sci 2008; 105(39):14832-7.
9. Luo HY, Wang YR, Miao LH, Yang PL, Shi PJ, Fang CX, Yao B, Fan YL. Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. Int J Syst Evol Microbiol. 2009; 59(4):863-8.
10. Jensen PR, Lauro FM. An assessment of actinobacterial diversity in the marine environment. Antonie Van Leeuwenhoek. 2008; 94(1):51-62.
11. Gulve RM, Deshmukh AM. Antimicrobial activity of the marine actinomycetes. Int Interdiscip Res J. 2012; 2(3).
12. Takizawa M, Colwell RR, Hill RT. Isolation and diversity of actinomycetes in the Chesapeake Bay. Appl Environ Microbiol. 1993; 59(4):997-1002.
13. Bertani LE, Bertani G. Genetics of P2 and related phages. Adv Genet 1971, (16): 199-237.
14. Salehi M, Hekmatdoost M, Hosseini F. Quinolone resistance associated with efllux pumps mexAB-oprM in clinical isolates of Pseudomonas aeruginosa. J Microbial World 2014, 6(4): 290-298.
15. Lister PD, Wolter DJ, Hanson ND. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin Microbiol Rev. 2009; 22(4):582-610.
16. Khosravi AD, Mihani F. Detection of metallo-β-lactamase–producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran. Diagnostic microbiology and infectious disease. 2008 Jan 1; 60(1):125-8.
17. Mohseni M, Norouzi H. Antifungal activity of actinomycetes isolated from sediments of the Caspian Sea. J Mazandaran Univ Med Sci. 2013; 23(104):80-7.
18. Xie JJ, Zhou F, Li EM, Jiang H, Du ZP, Lin R, Fang DS, Xu LY. FW523-3, a novel lipopeptide compound, induces apoptosis in cancer cells. Mol Med Rep. 2011; 4(4):759-63.
19. Songsumanus A, Kudo T, Igarashi Y, Tanasupawat S. Characterization and screening of antimicrobial activity of Micromonospora strains from Thai soils. Malays J Microbiol . 2013 Jan 1; 9(3):260-9.
20. Madhumita, T. Isolation and screening of micromonospora for bioactive metabolites and their bioevaluation. 2016.
21. Abdelmohsen UR, Pimentel-Elardo SM, Hanora A, Radwan M, Abou-El-Ela SH, Ahmed S, Hentschel U. Isolation, phylogenetic analysis and anti-infective activity screening of marine sponge-associated actinomycetes. Mar drugs. 2010; 8(3):399-412.
22. Valli S, Suvathi SS, Aysha OS, Nirmala P, Vinoth KP, Reena A. Antimicrobial potential of Actinomycetes species isolated from marine environment. Asian Pac J Trop Biomed. 2012; 2(6):469-73.
23. Sengupta, S, Pramanik, A, Ghosh, A, & Bhattacharyya, M. Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem. BMC microbiol. 2015; 15(1): 170.
24. Houge-Frydrych CS, Readshaw SA, Bell DJ. SB-219383, a novel tyrosyl tRNA synthetase inhibitor from a Micromonospora sp. J Antibiot. 2000; 53(4):351-6.
25. Ertaş M, Özdemir K, Atalan E. Isolation and characterization of Micromonospora bacteria from various soil samples obtained around Lake Van. Afr J Biotechnol. 2013;12(21).
26. Hernandez LM, BLANCO JA, Baz JP, Puentes JL, Millan FR, Vazquez FE, Fernandez-Chimeno RI, Gravalos DG. 4'-N-Methyl-5'-hydroxystaurosporine and 5'-Hydroxy staurosporine, New Indolocarbazole Alkaloids from a Marine Micromonospora sp. Strain. J Antibiot. 2000; 53(9):895-902.
27. Gutierrez-Lugo MT, Woldemichael GM, Singh MP, Suarez PA, Maiese WM, Montenegro G, Timmermann BN. Isolation of three new naturally occurring compounds from the culture of Micromonospora sp. P1068. Nat Prod Res. 2005; 19(7):645-52.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
