Volume 21, Issue 2 (5-2018)
J Arak Uni Med Sci 2018, 21(2): 75-85 |
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1- Department of Microbiology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran (IAUPS)
2- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , haddadi@kiau.ac.ir
3- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , haddadi@kiau.ac.ir
3- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract: (2793 Views)
Abstract
Background: In recent years, increasingly antibiotic resistance problem among Klebsiella isolates and side effects of antibiotics overuse have made researchers to study the antimicrobial activity of medicinal plants. The aim of this study was to study the inhibitory effect of ethanolic and aqueous extract of Vaccinium arctostaphylos on ESBLs producing Klebsiella strains.
Materials and Methods: Among 143 isolates of Klebsiella collected from some hospitals and clinical laboratories in Karaj, ESBLs producer were screened by phenotypic confirmatory test (PCT). One of them was identified based on 16S rRNA gene sequencing. MIC and MBC of ethanol and aqueous extracts of Vaccinium arctostaphylos were determined using microdilution method on selected ESBLs producing Klebsiella strains.
Results: Resistance to ceftazidime, ceftriaxon and cefotaxime was observed in 14.7% of the isolates. 32 isolates (22%) were detected as ESBL producers. Results of MIC and MBC tests showed that ethanolic and aqueous extract of Vaccinium arctostaphylos have inhibitory effect on ESBLs producing Klebsiella strains,
Conclusion: The presence of antibacterial activity could be confirmed in most plant species used in traditional medicine in Iran and we should focus on combining traditional medicines and modern drugs.
Background: In recent years, increasingly antibiotic resistance problem among Klebsiella isolates and side effects of antibiotics overuse have made researchers to study the antimicrobial activity of medicinal plants. The aim of this study was to study the inhibitory effect of ethanolic and aqueous extract of Vaccinium arctostaphylos on ESBLs producing Klebsiella strains.
Materials and Methods: Among 143 isolates of Klebsiella collected from some hospitals and clinical laboratories in Karaj, ESBLs producer were screened by phenotypic confirmatory test (PCT). One of them was identified based on 16S rRNA gene sequencing. MIC and MBC of ethanol and aqueous extracts of Vaccinium arctostaphylos were determined using microdilution method on selected ESBLs producing Klebsiella strains.
Results: Resistance to ceftazidime, ceftriaxon and cefotaxime was observed in 14.7% of the isolates. 32 isolates (22%) were detected as ESBL producers. Results of MIC and MBC tests showed that ethanolic and aqueous extract of Vaccinium arctostaphylos have inhibitory effect on ESBLs producing Klebsiella strains,
Conclusion: The presence of antibacterial activity could be confirmed in most plant species used in traditional medicine in Iran and we should focus on combining traditional medicines and modern drugs.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/12/23 | Accepted: 2018/04/29
Received: 2017/12/23 | Accepted: 2018/04/29
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