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Karymi F, Farhadie Langaraoudi, Poorfathollah A A, Jalali Khou H. Evaluation of Ploidy Status in Patients with Lymphoma (Non-Hodgkin and Hodkgin) by flow Cytometry with Paraffin-Embedded Tissue. J Arak Uni Med Sci 2000; 3 (3) :29-34
URL: http://jams.arakmu.ac.ir/article-1-6631-en.html

DNA analysis by flow cytometry is one of novel techniques recently introduce of utilize in routine clinical diagnosis laboratories , generally, there is an wverall agreement between DNA status and chromosomal content of cells, so with DNA analysis especially in neoplastic diseases, one coukd find the ploidy feature and number of cells which are in synthetic phase (S-phase fraction) and also cellular kinetics in select neoplasia, which are charactristics findings and factors that could be used as prognostic markers for predicting clinical behaviour and monitoring response to treatment protocol. In this study, we measured DNA content in eighty eight (88_ malignant lymphopreliferation diseases including 58 NHL and 30 HD diagnosed histopathologycally, by flow cytometry using Hedley method, Also immunohistochemical staining for proper immunophenotyping done in selected cases with ABC method with MoAb,s. Aneuploidy were 38% in NHL and 47% in HD patients. This occurance was unrelated to other parameters used as Age, tumor location and histologic subtypes. (P>0.05) S-phase fraction (SPF) measurement calculated as percentage of cellw in active phase and proligeration index determinations reveral higher frequency of SPF (PI) within Iranian patients rather than reported Western patients. This finding can account for lower survival rate and poor response to chemotherapy and (or radiotherapeutic regimen used in Iranian patients, but also other parameters in this regard also should be considered simultaneously.