Volume 7, Issue 2 (Summer 2004)
J Arak Uni Med Sci 2004, 7(2): 48-57 |
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Mosayebi G, Moazzani M. Comparison of Spleen and Live Dendritic Cell of Mouse in Induction of Lymphoproliferative Response. J Arak Uni Med Sci 2004; 7 (2) :48-57
URL: http://jams.arakmu.ac.ir/article-1-6783-en.html
URL: http://jams.arakmu.ac.ir/article-1-6783-en.html
Abstract: (817 Views)
Introduction: Dendritic cells (DCs) play an important role in induction of cellular immune responses. It has been showed that the nature of the immune responses is not the same in all tissues. It seems that DCs reside in different organs may distinct in their ability to induce lymphocyte proliferation .The innate tolerogenic characteristic of the liver may be due to the inability of liver DCs to induce proliferative responses.
Materials and Methods: In this study, the DCs were isolated from the liver and spleen of normal C57BL/6 mice by enzymatic digestion and nycodenz centrifugation gradient. Isolated DCs were pulsed with a proper concentration of myelin oligodendrocyte glycoprotein peptide (MOG35-55). About 6*105 pulsed DCs from liver and spleen were injected to the footpad of two different groups of mice. Unpulsed Dcs were injected to control group. After 5 days, the mononuclear cells (MNCs) of the popliteal lymph nodes were isolated from immunized mice and their proliferative response were evaluated in the presence and absence of MOG35-55.
Results: The obtained results showed that the proliferartive response intrnsity of MNGs in mice immunized with pulsed DCs were higher than control group (p<0.004). furthermore there was no significant difference between proliferative response of mice immunized with liver DCs and those immunized with splenic DCs. These finding showed that the liver and spleen DCs could be pulsed proplerly with the antigen in tissue culture and can induce a reasonable proliferative response.
Conclusion: The equal ability of liver and spleen DCs to induce the proliferative response indicates that the type of induced response may differ at in vivo and ex vivo conditions and microenviromental factors can modulated the immune response.
Materials and Methods: In this study, the DCs were isolated from the liver and spleen of normal C57BL/6 mice by enzymatic digestion and nycodenz centrifugation gradient. Isolated DCs were pulsed with a proper concentration of myelin oligodendrocyte glycoprotein peptide (MOG35-55). About 6*105 pulsed DCs from liver and spleen were injected to the footpad of two different groups of mice. Unpulsed Dcs were injected to control group. After 5 days, the mononuclear cells (MNCs) of the popliteal lymph nodes were isolated from immunized mice and their proliferative response were evaluated in the presence and absence of MOG35-55.
Results: The obtained results showed that the proliferartive response intrnsity of MNGs in mice immunized with pulsed DCs were higher than control group (p<0.004). furthermore there was no significant difference between proliferative response of mice immunized with liver DCs and those immunized with splenic DCs. These finding showed that the liver and spleen DCs could be pulsed proplerly with the antigen in tissue culture and can induce a reasonable proliferative response.
Conclusion: The equal ability of liver and spleen DCs to induce the proliferative response indicates that the type of induced response may differ at in vivo and ex vivo conditions and microenviromental factors can modulated the immune response.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2021/01/27 | Accepted: 2004/06/30
Received: 2021/01/27 | Accepted: 2004/06/30
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