Volume 7, Issue 4 (Winter 2004)
J Arak Uni Med Sci 2004, 7(4): 14-21 |
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Shariatzadeh M A, Soleimani M, Dezfulian A, Noori M, Roodi E. The Stereologic Study of Onion Water-Alcohol Extract on Diabetic Rats Kidneys. J Arak Uni Med Sci 2004; 7 (4) :14-21
URL: http://jams.arakmu.ac.ir/article-1-6810-en.html
URL: http://jams.arakmu.ac.ir/article-1-6810-en.html
Abstract: (895 Views)
Introduction: Diabetes during its progress can result in nephropathy. Some of its symptoms are increasing kidney size and weight and its components. Regarding useful components of onion and its antioxidant, decreasing stress oxidative and b decreasing blood glucose effects. This investigation is designed to study the effect of onion water-alcohol extract in preventing nephropathy and its effect on kidney structure based on stereology method.
Materials and Methods: Four groups of matured vistar rats (n=8) were selected randomly (control group, control + extract group, diabetic only group, diabetic + extract group). Diabetes was induced by injecting interperitoneal sreptozotocin (60mg/kg). The control+ extract group and diabetic + extract group were treated by onion-water extract (50 mg/rat)every day for four weeks. Then all groups were anesthetized and their left kidney was removed and fixed in Bouin fixative. After histologic passage and H & E dying, using stereologic techniques, qualitative measurement was performed by Cavalier method for cortex, medulla, glomerulus and kidney size. Data analysis was done by SPSS software using one way anova, tukey and paired test. p<0.05 was considered significant.
Results: The primary and secondary weight of rats in only diabetic group and diabetic + extract group was not different, but in control group and control + extract group was significantly different (p<0.05). Medulla, cortex and whole kidney size in only diabetic group in comparison with diabetic+ extract group had no difference, but total glomerular size in diabetic only group and diabetic + extract group was significantly different (p<0.001).
Conclusion: Experimental induction of diabetes by STZ in a short period showed that onion extract can prevent glomerular hypertrophy and increasing kidney weight in diabetic rats, but had no effect on overall kidney size. So the study of onion extract effects on kidney structure during a long period is recommended.
Materials and Methods: Four groups of matured vistar rats (n=8) were selected randomly (control group, control + extract group, diabetic only group, diabetic + extract group). Diabetes was induced by injecting interperitoneal sreptozotocin (60mg/kg). The control+ extract group and diabetic + extract group were treated by onion-water extract (50 mg/rat)every day for four weeks. Then all groups were anesthetized and their left kidney was removed and fixed in Bouin fixative. After histologic passage and H & E dying, using stereologic techniques, qualitative measurement was performed by Cavalier method for cortex, medulla, glomerulus and kidney size. Data analysis was done by SPSS software using one way anova, tukey and paired test. p<0.05 was considered significant.
Results: The primary and secondary weight of rats in only diabetic group and diabetic + extract group was not different, but in control group and control + extract group was significantly different (p<0.05). Medulla, cortex and whole kidney size in only diabetic group in comparison with diabetic+ extract group had no difference, but total glomerular size in diabetic only group and diabetic + extract group was significantly different (p<0.001).
Conclusion: Experimental induction of diabetes by STZ in a short period showed that onion extract can prevent glomerular hypertrophy and increasing kidney weight in diabetic rats, but had no effect on overall kidney size. So the study of onion extract effects on kidney structure during a long period is recommended.
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Received: 2021/01/29 | Accepted: 2004/12/30
Received: 2021/01/29 | Accepted: 2004/12/30
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