TY - JOUR T1 - Indole-3-Carbinol Enhances Doxorubicin-Induced Apoptosis Through Suppression of NF-B in B-Cell Precursor Acute Lymphoblastic Leukemia Cell Line NALM-6 TT - افزایش القاء آپوپتوز ناشی از دوکسوروبیسین توسط ایندول تری کربینول با مهار مسیر پیام دهی فاکتور هسته‌ای کاپا-B در رده سلولی لوسمی لنفوبلاستیک حاد پیش ساز سلولB (NALM-6) JF - HBI_Journals JO - HBI_Journals VL - 17 IS - 11 UR - http://jams.arakmu.ac.ir/article-1-3098-en.html Y1 - 2015 SP - 37 EP - 52 KW - Doxorubicin KW - I3C KW - NF-B KW - Pre-B acute lymphoblastic leukemia N2 - Background: Doxorubicin is a chemotherapeutic agent still in widespread use in hematologic malignancies. A side effect of anthracyclines such as doxorubicin is the activation of nuclear factor-&kappaB (NF-&kappaB), a potent inducer of antiapoptotic genes, which may blunt the therapeutic efficacy of the drugs. In this study, the effect of indole -3-carbinol (I3C) on the activation NF-&kappaB and the anti-apoptotic genes whose expression is regulated by NF-&kappaB was assessed in NALM-6 cells. Materials and Methods: NALM-6 cells were preincubated with various concentrations of I3C and then treated with doxorubicin. Cellular DNA content assay and Annexin V-FITC staining were performed by flowcytometry for evaluation of apoptosis. For assessing the effect of I3C on the expression of XIAP, survivin, and nuclear p65 proteins, NALM-6 cells were pretreated with I3C and then incubated with doxorubicin. Whole-cell and nuclear extracts were prepared for Western blot analysis. A paired t-test was conducted to evaluate the results. Results: DNA histogram analysis of NALM-6 cells indicates a combination of I3C with doxorubicin significantly escalated the percentages of sub-G1 population cells compared with doxorubicin - only treated group (p M3 ER -