%0 Journal Article %A Salesi, Mohsen %A Mehrtash, Mohammad %A Daryanoosh, Farhad %A Tanide, Nader %T The Role of Caloric Restriction on Lipid Coat Proteins Gene Expression and Insulin Resistance after 8 Weeks High Caloric Diet in Male Rats %J Journal of Arak University of Medical Sciences %V 21 %N 5 %U http://jams.arakmu.ac.ir/article-1-5604-en.html %R %D 2018 %K Calorie restriction, Insulin resistance, Lipid coat proteins, Perilipin 1 and 5, %X Background and Aim: Excessive fat accumulation is one of the most important mechanisms of type 2 diabetes and insulin resistance. Accordingly, in this study, we will examine the effect of caloric restriction on lipid coat proteins that prevent lipolysis of the adipose tissue. Materials and Methods: In this study, 30 adult male Sprague-Dawley rats (200 ± 20 weight) were used. First, rats were divided into 2 groups (standard and high-calorie diet). After 8 weeks, the high-calorie diet group was randomly assigned to two subgroups: caloric restriction and high-fat diet. Finally, the amount of perilipin 1 and 5 genes expression evaluated by Pcr and insulin resistance evaluated by HOMA index. Findings: The weight and insulin resistance of the high-fat diet group was significantly higher than the standard and calorie restriction group (p≤0.005). Insulin resistance was not significantly different between the caloric restriction and standard group (p = 0.394). The expression of perilipin 1 in the adipose tissue of the high-fat diet was significantly higher than the caloric restriction group (p ≤0.005). The expression of perilipin 1 gene in skeletal muscle in the high-fat diet group was significantly higher than the calorie restriction and standard diet groups (p≤0.005). The calorie restriction group had more perilipin 5 expressions than the high-fat diet and the standard group (p ≤0.005). Conclusion: The caloric restriction may be due to the effect on lipid coat proteins and, as a result, a decrease in fat accumulation, that it can prevent insulin resistance. %> http://jams.arakmu.ac.ir/article-1-5604-en.pdf %P 21-31 %& 21 %! %9 Original Atricle %L A-10-4622-2 %+ Department of Exercise Physiology, Shiraz University, Shiraz, Iran. %G eng %@ 1735-5338 %[ 2018