TY - JOUR JF - HBI_Journals JO - J Arak Uni Med Sci VL - 21 IS - 5 PY - 2018 Y1 - 2018/10/01 TI - The Role of Caloric Restriction on Lipid Coat Proteins Gene Expression and Insulin Resistance after 8 Weeks High Caloric Diet in Male Rats TT - نقش محدودیت کالری بر بیان ژن پروتئین‌های پوشش‌دهنده بافت چربی و مقاومت به انسولین پس از 8 هفته رژیم غذایی پرکالری در رت‌های نر N2 - Background and Aim: Excessive fat accumulation is one of the most important mechanisms of type 2 diabetes and insulin resistance. Accordingly, in this study, we will examine the effect of caloric restriction on lipid coat proteins that prevent lipolysis of the adipose tissue. Materials and Methods: In this study, 30 adult male Sprague-Dawley rats (200 ± 20 weight) were used. First, rats were divided into 2 groups (standard and high-calorie diet). After 8 weeks, the high-calorie diet group was randomly assigned to two subgroups: caloric restriction and high-fat diet. Finally, the amount of perilipin 1 and 5 genes expression evaluated by Pcr and insulin resistance evaluated by HOMA index. Findings: The weight and insulin resistance of the high-fat diet group was significantly higher than the standard and calorie restriction group (p≤0.005). Insulin resistance was not significantly different between the caloric restriction and standard group (p = 0.394). The expression of perilipin 1 in the adipose tissue of the high-fat diet was significantly higher than the caloric restriction group (p ≤0.005). The expression of perilipin 1 gene in skeletal muscle in the high-fat diet group was significantly higher than the calorie restriction and standard diet groups (p≤0.005). The calorie restriction group had more perilipin 5 expressions than the high-fat diet and the standard group (p ≤0.005). Conclusion: The caloric restriction may be due to the effect on lipid coat proteins and, as a result, a decrease in fat accumulation, that it can prevent insulin resistance. SP - 21 EP - 31 AU - Salesi, Mohsen AU - Mehrtash, Mohammad AU - Daryanoosh, Farhad AU - Tanide, Nader AD - Department of Exercise Physiology, Shiraz University, Shiraz, Iran. KW - Calorie restriction KW - Insulin resistance KW - Lipid coat proteins KW - Perilipin 1 and 5 UR - http://jams.arakmu.ac.ir/article-1-5604-en.html ER -