fa بررسی ارتباط بین پلی‌مورفیسم 155G/C ژن CGB5 با نتیجه‌ی لقاح مصنوعی- انقال جنین در جمعیت ایران علوم پایه Basic Sciences پژوهشي اصیل Original Atricle <div style="text-align: justify;"><strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;">چکیده </span></span></strong><br> <strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;">زمینه و هدف:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> حاملگی موفق به توانایی رویان در تکثیر سلول&shy;های تروفوبلاست و تهاجم به آندومتر مادر و هم&shy;چنین</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">القای جریان خون خود پس از لانه&shy;گزینی بستگی دارد. گنادوتروپین جفتی انسان بتا (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&beta;-hCG</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">)، لانه&shy;گزینی بلاستوسیت، رگ&shy;زایی رحم و آنژیژنز را افزایش می&shy;دهد، هم&shy;چنین نگهداری ثبات رحم و سازگاری ایمنی در دوران بارداری را تنظیم می&shy;کند. زیر واحد بتا </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">hCG</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> توسط ژن&shy;های </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB3</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB6</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB7</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB8</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> رمزگذاری می شود. هدف از این مطالعه بررسی ارتباط پلی&shy;مورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5-155G/C</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> و نتایج بالینی در زنان تحت درمان با </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IVF-ET</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> ، بود.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;">مواد و روش&shy; ها:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> این مطالعه بر روی 200 بیمار که تحت درمان با </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IVF-ET</span></span></span> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">)</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">شامل100 نفر با نتیجه مثبت و 100 نفر با نتیجه منفی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&nbsp;(IVF-ET</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">قرار گرفتند، صورت گرفت</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> ژنوتیپ</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5 </span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">&nbsp;در جایگاه </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">-155G/C</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> توسط واکنش زنجیره‌ای پلی‌مراز-پلی‌مورفیسم طول قطعه محدود کننده (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">PCR-RFLP</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">) مورد</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">بررسی</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">قرار</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">گرفت. تجزیه و تحلیل</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">آماری</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">کمک</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">نرم</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">افزار</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">مدکالک</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">انجام</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">شد.</span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;">یافته&shy; ها:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> نتایج نشان می&shy;دهد که ژنوتیپ</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CC </span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">&nbsp;پلی&shy;مورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5-155G/C</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> با کاهش خطر شکست </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IVF-ET</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> مرتبط است(02/0 =</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">، 29/0=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">OR</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">، 85/0-1/0=</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CI</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">95%). اگرچه، تفاوت معناداری بین توزیع آللی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5-155G/C</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> بین دو گروه وجود نداشت (46/1 =²</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">x</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> ، 22/0 = </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">p</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">)</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><br> <strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;">نتیجه&shy; گیری:</span></span></strong><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> نتایج این مطالعه حاکی از این است</span></span> <span style="font-family:b mitra;"><span style="font-size:12.0pt;">که ژنوتیپ</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CC(-155G/C) </span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;">&nbsp;ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> اثر حفاظتی در نتیجه&shy;ی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IVF-ET</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> دارد. مطالعات بیشتر با اندازه&shy; ی بزرگ&shy;تر نمونه ها در جمعیت&shy;های مختلف، جهت شناخت مکانیسم های دخیل در پیوستگی پلی&shy;مورفیسم&shy;های ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CGB5</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> و نتیجه ی </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">IVF-ET</span></span></span><span style="font-family:b mitra;"><span style="font-size:12.0pt;"> ضروری می باشد. </span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family: times new roman;"><strong>Abstract</strong><br> <strong><em>Background:</em></strong> Successful pregnancy depends on the ability of the embryo to achieve appropriate extent of trophoblastic proliferation and invasion into maternal endometrium as well as, once implanted, to induce its own blood supply. Beta Human chorionic gonadotropin (&beta;-hCG), enhances blastocyst implantation, uterine vascularization, and angiogenesis, as well as regulates maintenance of uterine quiescence and immunological adaptation during pregnancy. The &beta;-subunit of hCG is encoded by CGB3, CGB6, CGB5, CGB7 and CGB8 genes. The aim of this study was to evaluate the association of CGB5-G/C polymorphism and the clinical outcomes in women who underwent IVF-ET procedures.<br> <strong><em>Materials and Methods:</em></strong> A total of 200 patients undergoing IVF-ET (100 patients with positive and 100 patients with negative IVF-ET outcome) were included in this study. Genotyping of CGB5 at -155G/C polymorphic site was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Statistical analysis was performed using the MedCalc software.<br> <strong><em>Results:</em></strong> Our findings show that the CC genotype of the CGB5 -155G/C polymorphism is associated with decreased risk of IVF-ET failure (OR=0.29; 95%CI=0.1-0.85; p=0.02). However, the allelic distribution of the CGB5 -155G/C is not significantly different between two groups (&chi;2=1.46; p=0.22).<br> <strong><em>Conclusion:</em></strong> The results of this study suggested that CGB5 (-155G/C) CC genotype has a protective effect on IVF-ET outcome. More studies with larger sample sizes on different populations are necessary to elucidate the underlying mechanisms which can explain the associations found between the GGB5 gene polymorphisms and IVF-ET outcome.</span></span><br> &nbsp;</div> لقاح مصنوعی, پلی‌مورفیسم, CGB5 CGB5, IVF-ET, Polymorphism 11 19 http://jams.arakmu.ac.ir/browse.php?a_code=A-10-4961-2&slc_lang=fa&sid=1 Bahareh Babaei Houlari بهاره بابائی هولاری Bahare.babaei@yahoo.com 4600319475328460058647 4600319475328460058647 No Department of Genetics, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran گروه ژنتیک، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Zivar Salehi زیور صالحی geneticzs@yahoo.co.uk 4600319475328460058648 4600319475328460058648 Yes Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران