fa تأثیر یک دوره بی‌تحرکی اندام تحتانی بر بیان برخی ژن‌های درگیر در تنظیم فرآیندهای میتوکندریایی عضله‌ی نعلی رت‌های تمرین کرده و بی‌تمرین عمومى General پژوهشي اصیل Original Atricle <div style="text-align: justify;"><strong><span style="color:#12336E;"><span style="font-family:b nazanin;">زمینه و هدف:</span></span></strong><span style="font-family:b nazanin;"> هدف از پژوهش حاضر، بررسی تأثیر یک دوره بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی اندام تحتانی بر بیان ژن</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">های </span><span dir="LTR"><span style="font-family:times new roman,serif;">PGC-1&alpha;</span></span><span style="font-family:b nazanin;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;">NRF1</span></span><span style="font-family:b nazanin;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;">Mfn2</span></span><span style="font-family:b nazanin;">، </span><span dir="LTR"><span style="font-family:times new roman,serif;">PINK1</span></span><span style="font-family:b nazanin;"> و</span><span dir="LTR"><span style="font-family:times new roman,serif;"> Drp1</span></span><span style="font-family:b nazanin;">به عنوان تنظیم</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">گرهای اصلی کیفیت و عملکرد میتوکندریایی در عضله</span> <span style="font-family:b nazanin;">نعلی رت</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">های تمرین کرده استقامتی بود. </span><br> <strong><span style="color:#12336E;"><span style="font-family:b nazanin;">مواد و روش</span></span></strong><strong><span dir="LTR"><span style="color:#12336E;"><span style="font-family:times new roman,serif;">‌</span></span></span></strong><strong><span style="color:#12336E;"><span style="font-family:b nazanin;">ها:</span></span></strong><span style="font-family:b nazanin;"> به همین منظور 15 سر رت نر اسپراگ داولی به سه گروه (کنترل، ورزش+ بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی و گروه بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی) تقسیم شدند. رت</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">های گروه ورزش+ بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی طی 12 هفته و هر هفته 5 جلسه بر روی تردمیل دویدند. اندام تحتانی حیوانات برای 7 روز با روش قالب</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">گیری بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرک شد. عضله</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">ی نعلی استخراج و میزان بیان ژن</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">های موردنظر به روش </span><span dir="LTR"><span style="font-family:times new roman,serif;">RT-PCR</span></span><span style="font-family:b nazanin;"> اندازه</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">گیری گردید. جهت تعیین تفاوت</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">ها از روش آنوای یک</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">طرفه و آزمون تعقیبی توکی در سطح معنی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">داری 05/0</span><span style="font-family:sakkal majalla;">&ge;</span><span dir="LTR"><span style="font-family:times new roman,serif;">&alpha;</span></span><span style="font-family:b nazanin;"> استفاده شد. </span><br> <strong><span style="color:#12336E;"><span style="font-family:b nazanin;">ملاحظات اخلاقی:</span></span></strong> <span style="font-family:b nazanin;">این مطالعه با کد اخلاق </span><span dir="LTR"><span style="font-family:times new roman,serif;">IR.SUMS.REC.1396.S444</span></span><span style="font-family:b nazanin;"> به تصویب کمیته اخلاق پژوهشی دانشگاه علوم پزشکی شیراز رسیده است.</span><br> <strong><span style="color:#12336E;"><span style="font-family:b nazanin;">یافته</span></span></strong><strong><span dir="LTR"><span style="color:#12336E;"><span style="font-family:times new roman,serif;">‌</span></span></span></strong><strong><span style="color:#12336E;"><span style="font-family:b nazanin;">ها:</span></span></strong><span style="font-family:b nazanin;"> نتایج نشان داد که بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی در هر دو گروه بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی و ورزش + بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی نسبت به گروه کنترل موجب کاهش بیان ژن&shy;های </span><span dir="LTR"><span style="font-family:times new roman,serif;">PGC-1&alpha;</span></span><span style="font-family:b nazanin;"> (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;"> و 045/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">)، </span><span dir="LTR"><span style="font-family:times new roman,serif;">NRF1</span></span><span style="font-family:b nazanin;"> (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;"> و 006/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">)، </span><span dir="LTR"><span style="font-family:times new roman,serif;">Mfn2</span></span><span style="font-family:b nazanin;"> (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;"> و 001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">) و افزایش </span><span dir="LTR"><span style="font-family:times new roman,serif;">PINK1</span></span><span style="font-family:b nazanin;"> (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;"> و 001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">) می</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">شود، اما بیان ژن </span><span dir="LTR"><span style="font-family:times new roman,serif;">Drp1</span></span><span style="font-family:b nazanin;"> تغییر معناداری نداشت (069/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;"> و 223/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">). هم‌چنین بررسی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">ها مشخص کرد که در گروه ورزش + بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی، کاهش بیان </span><span dir="LTR"><span style="font-family:times new roman,serif;">PGC-1&alpha;</span></span><span style="font-family:b nazanin;"> (013/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">)، </span><span dir="LTR"><span style="font-family:times new roman,serif;">NRF1</span></span><span style="font-family:b nazanin;"> (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">) و </span><span dir="LTR"><span style="font-family:times new roman,serif;">Mfn2</span></span><span style="font-family:b nazanin;"> (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">) کمتر از گروه بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی بود. هم‌چنین افزایش بیان </span><span dir="LTR"><span style="font-family:times new roman,serif;">PINK1</span></span><span style="font-family:b nazanin;"> نیز کمتر از گروه بی</span><span dir="LTR"><span style="font-family:times new roman,serif;">‌</span></span><span style="font-family:b nazanin;">تحرکی بود (001/0</span><span dir="LTR"><span style="font-family:times new roman,serif;">p=</span></span><span style="font-family:b nazanin;">). </span><br> <strong><span dir="RTL"><span style="color:#12336E;"><span style="font-family:b nazanin;"><span style="font-size:11.0pt;">نتیجه</span></span></span></span><span style="color:#12336E;"><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">‌</span></span></span><span dir="RTL"><span style="color:#12336E;"><span style="font-family:b nazanin;"><span style="font-size:11.0pt;">گیری:</span></span></span></span></strong><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:11.0pt;"> این مطالعه نشان می</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:11.0pt;">دهد تمرین استقامتی اثر محافظتی بر کیفیت میتوکندریایی در دوره بی</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:11.0pt;">تحرکی دارد، اما نمی</span></span></span><span style="font-family:times new roman,serif;"><span style="font-size:11.0pt;">‌</span></span><span dir="RTL"><span style="font-family:b nazanin;"><span style="font-size:11.0pt;">تواند از بروز نقص میتوکندریایی جلوگیری کند.</span></span></span></div> <div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background and Aim:</strong> The purpose of this study was to determine the effect of a hind limb immobilization on the expression of PGC-1&alpha;, NRF1, Mfn2, PINK1 and Drp1 genes as the main regulators of mitochondrial quality and function in soleus muscle of endurance trained rats.<br> <strong>Materials and Methods:</strong> In this study, 15 male Sprague-Dawley rats were divided into three groups (control, exercise +immobilization and immobilization). The exercise + immobilization group run on the treadmill for 12 weeks and five times per week. The hind limb of the animal was immobilized for seven days with the casting method. Soleus muscle was extracted and the expression of the genes was measured by RT-PCR method. Univariate ANOVA and Tukey post hoc test were used to determine the differences (&alpha; = 0.05).<br> <strong>Ethical Considerations:</strong> This study with research ethics code IR.SUMS.REC.1396.S444 has been approved by research ethics committee at Shiraz university of medical sciences, Iran.<br> <strong>Findings:</strong> Results showed that immobilization in both immobilization and exercise +immobilization groups, compared to the&nbsp; conterl group, reduced the expression of PGC-1&alpha;&nbsp; gene (p = 0.001 and p = 0.045), NRF1 (p = 0.001 and p = 0.006), Mfn2 (p = 0.001, p = 0.001) and increased the expression of PINK1 (p = 0.001 and p= 0.001), but the expression of Drp1 gene didn&#39;t change significantly (p = 0.069 and p = 0.223). Also, studies showed that the expression of PGC-1&alpha; (p = 0.013), NRF1 (p = 0.001) and Mfn2 (p = 0.001) in the exercise + immobilization was lower in compare with the immobilization group. The expression of PINK1 was lower than immobilization group as well (p = 0.001).<br> <strong>Conclusion:</strong> This study shows that endurance training has a protective effect on mitochondrial quality during the immobilization period, but it can&#39;t prevent mitochondrial dysfunction. </span></div> تمرین استقامتی, عدم استفاده عضلانی, نقص عملکردی میتوکندریایی, NRF1 mRNA, PGC-1α mRNA Endurance training, Mitochondrial dysfunction , Muscle disuse, NRF1 mRNA, PGC-1α mRNA 51 61 http://jams.arakmu.ac.ir/browse.php?a_code=A-10-5672-1&slc_lang=fa&sid=1 Vahid Hadidi وحید حدیدی ahad.shafie312@yahoo.com 4600319475328460066494 4600319475328460066494 No Department of Exercise Physiology, Faculty of Psychology and Educational Science, Shiraz University, Shiraz, Iran. گروه فیزیولوژی ورزشی، دانشکده روانشناسی و علوم تربیتی، دانشگاه شیراز، شیراز، ایران. Farhad Daryanoosh فرهاد دریانوش daryanoosh@shirazu.ac.ir 4600319475328460066495 4600319475328460066495 Yes Department of Exercise Physiology, Faculty of Psychology and Educational Science, Shiraz University, Shiraz, Iran. گروه فیزیولوژی ورزشی، دانشکده روانشناسی و علوم تربیتی، دانشگاه شیراز، شیراز، ایران. Javad Nemati جواد نعمتی ahad.shafie312@yahoo.com 4600319475328460066496 4600319475328460066496 No Department of Exercise Physiology, Faculty of Psychology and Educational Science, Shiraz University, Shiraz, Iran. گروه فیزیولوژی ورزشی، دانشکده روانشناسی و علوم تربیتی، دانشگاه شیراز، شیراز، ایران. Nader Tanideh نادر تنیده ahad.shafie312@yahoo.com 4600319475328460066497 4600319475328460066497 No Stem Cell Technology Research Center, Department of Pharmacology, Shiraz University of Medicine Science, Shiraz, Iran. مرکز تحقیقات فن‌آوری سلول‌های بنیادی، بخش فارماکولوژی، دانشگاه علوم پزشکی شیراز، شیراز ایران.