46 1735-5338 Arak University of Medical Sciences 3337 Basic Sciences A Molecular Study of the Prevalence of Pathogenicity Island type 2 in strains of Pseudomonas Aeruginosa Isolated from Environmrntal and Clinical Samples inTehran Hospitals, 2013 Abdanankord Yasamin b Dabiri Hossein c Goodarzi Hossein d b Department of Microbiology, Fars Sciences and Research Branch, Islamic Azad University, Shiraz, Iran c Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran d Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran 1 9 2015 18 6 1 7 07 12 2014 23 06 2015 Background: Pseudomonas aeruginosa is one of the important causes of hospital infections septicemia, in patients hospitalized in burn ward and those with cystic fibrosis. Considering the PAPI-2 important role in bacteria pathogenesis, the aim of this study is to investigate the frequency of the encoding genes exoU and xerC as markers of PAPI-2 from isolated environmental and clinical samples. Materials and Methods: In this descriptive study, 40 isolates from sewage of burn wound hospital and 30 from patients hospitalized in burn ward of the hospital covered by shahid Beheshti University, respectively. The frequency of PAPI-2 in both environmental and clinical strains was detected by using PCR and the primers exou and xerc. Results: Of 40 studied environmental pseudominas aeruginosa strains that their genus and species were confirmed by chemical tests, 30 samples (75%) consisted of exoU gene and 32(80%) included xerC gene. Also, of 30 isolated strains of burn patients, 23 isolates(76.7%) contained both exoU and xerC gene. The results revealed a high prevalence of PAPI-2 (90%) between clincial and environmental samples of pseudomonas aeruginosa. Conclusion: With due attention to the results, information reveal that the importance and prevalence of pathogenicity island type 2 were high in Iranian clinical and environmental Pseudomonas aeruginosa isolates. Also, considering all environmental isolates have at least one of genes, we should care about the risk of transporting pathogenic strains and find solutions to control it.
3520 Health Studying the Polypropylenimine-G2 (PPI-G2) Dendrimer Performance in Removal of Escherichia coli, Proteus Mirabilis, Bacillus Subtilis and Staphylococcus Aureus from Aqueous Solution Izanloo Hasan e Nazari Shahram f Ahmadi Jebelli Mohammad g Alizadeh Matboo Soodabeh h Tashauoei Hamid Reza i Vakili Behnam j Rajabi Mohammad k Aghababaei Hosein l Majidi Gharib m e Department of Environmental Health Engineering, Research Center for Environmental Pollutants, Qom University of Medical Sciences, Qom, Iran f Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran g Research Center for Environmental Health, School of Public Health, Kurdistan University of Medical Sciences, Sanandaj, Iran h Ardabil University of Medical Sciences, Ardabil, Iran i Department of Environmental Health Engineering, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran j Water & Wastewater Engineering Company, Tehran, Iran k Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran. l Science and Research Branch, Islamic Azad University, Tehran, Iran m Qom University of Medical Sciences, Qom, Iran 1 9 2015 18 6 8 16 20 02 2015 06 05 2015         Background: Dendrimers are a subset of branched structures that have certain structural order. The aim of this study was to investigate the performance of Polypropylenimine-G2 (PPI-G2) dendrimers in removal of Escherichia coli, Proteus mirabilis, Bacillus subtilis and Staphylococcus aureus from aqueous solution .         Materials and Methods: In this experimental study , initially dilution of 103 CFU/ml was prepared from each strain of bacteria . Then , different concentrations of dendrimers (0.5, 5, 50 and 500 µg/ml) was added to water . In order to determine the efficiency of dendrimers in removal of bacteria, samples were taken at different times (0, 10, 20, 30, 40, 50 and 60 min) and were cultured on nutrient agar medium . Samples were incubated for 24 hours at 37 ° C and then the number of colonies was counted .        Results: By the increasment of dendrimer concentration and contact time , the number of bacteria in aqueous solution decreased. In times of 40 , 50 and 60 minutes, and the concentrations of 50 and 500 µg/ml, all kinds of bacteria in aqueous solution were removed . 0.5 µg/ml of dendrimer concentration had not effect in reducing the number of Escherichia coli and Proteus mirabilis . The effect of dendrimer on gram-negative bacteria was weaker than gram-positive bacteria.        Conclusion: Results of this study indicated that PP I -G2 dendrimer is able to remove Escherichia coli , Proteus mirabilis , Staphylococcus aureus and Bacillus subtilis in aqueous solution. However, using dendrimers can be considered as a new approach for drinking water disinfection but it requires further wide range studies. 3423 Basic Sciences The Effect of Prenatal Stress on Seizure Threshold and Histomorphometrical Changes on Cerebellar Cortex in NMRI Mice Parsaee Sahar n Mohseni Kochesfehani Homa o Kaka Gholamreza p Sadraee Homayon Kahali Mojtaba n Department of Animal Sciences, Kharazmi University, Tehran, Iran o Department of Biology, Kharazmi University, Tehran, Iran. p Neurosciences Research center, Baqiyatallah University of Medical Sciences, Tehran, Iran Neurosciences Research center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Neurosciences Research center, Baqiyatallah University of Medical Sciences, Tehran, Iran. 1 9 2015 18 6 17 26 13 01 2015 23 06 2015   Background: Stress is a mental or emotional disturbance that occurs in response to external stimuli and can also appear during pregnancy. The aim of this study is to investigate the effect of maternal stress during pregnancy on the cerebellar structure changes and seizure threshold of their offspring .   Materials and Methods: In this experimental study, 20 pregnant female rats were divided into two groups: 1) Non stress group, and 2) Stress group which were under immobilization stress one hour for 14 days . The seizure threshold test in offspring was performed by injecting Pantilen tetrazol drug (PTZ)(n=8) . To investigate the cerebellum development, the offspring were divided into three groups . Control group: mothers did not any stress and offspring did not receive PTZ (n=4). Sham group: mothers did not stress but the offspring had received PTZ(n=4). Experimental group: mothers did stress and offispring did receive PTZ(n=4). After the section of cerebellum, the thickness of cerebellum layers and the number of cells in each layer were evaluated.   Results: The mean of seizure threshold in the offspring whose mothers were under the stress of pregnancy significantly increased compared to children whose mothers no received stress (p<0.001). In the other side, mean number of purkinje cells in the experimental group significantly decreased compared with the other groups (p<0.001). No significant differences were found in the mean of granular and molecular layers thickness of cerebellum in the experimental group when compared with the other groups(p<0.05). However, mean cellular density in the granular layer of cerebellum in the experimental group significantly decreased compared to other groups (p<0.001). Conclusion: Stress during pregnancy increased the seizure threshold in offspring and caused some developmental and structural disorders in the cerebellar rat offspring. 3331 General Success and Complications of Trabeculectomy with Releasable Sutures and in Open Angle Glaucoma Treatment Sarvarian Ahmad Department of Ophthalmology, Amirkabir Hospital, Arak 1 9 2015 18 6 27 32 06 12 2014 22 08 2015   Background: Trabeculectomy is one of the most common ophthalmic surgeries, but over filtration may cause serious ophthalmic complications. The aim of this study is to compare two suture methods in trabeculectomy.   Materials and Methods: This clinical trial accomplished on 28 eyes of 28 patients with primary open angle glaucoma unresponsive to full medication. Patients were divided into two equal groups. In one group, standard and the other group releasable suture trabeculectomy was performed. Post-Surgery short-term complications and intraocular pressure were compared in both groups.   Results: The mean IOP reduction in group with releasable sutures was 22.14±13.99 and in standard group was 28.14±14.10 (p=0.269). Frequency of iridocorneal touch in releasable and standard groups was 7.1 and 50 percent, respectively(p=0.016). Also, Frequency of shallow anterior chamber in releasable and standard groups was 21.4 and 92.2 percent, respectively(p<0.001). Conclusion: Trabeculectomy with releasable suture reduces short-term complications and in this group, IOP control is equal to standard trabeculectomy. 3461 General The Effects of Melatonin with Memantin on MPTP-Induced Parkinson Model in Male Mice Talebi Reza Alimoradian Abbas Sadegh Mehdi Department of Anatomy, Arak University of Medical Sciences, Arak, Iran Department of Pharmacology, Arak University of Medical Sciences, Arak, Iran Department of Physiology, Arak University of Medical Sciences, Arak, Iran 1 9 2015 18 6 33 41 26 01 2015 06 05 2015   Background: Oxidative stress and severe neuro-excitation have significant effects on pathogenesis of Parkinson’s disease and agents with antioxidant property can potentially prevent these effects. Herein we examined potential protective effects of melatonin as an antioxidant agent and memantine as an uncompetitive receptor of NMDA, on a model of Parkinson’s disease induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP).   Materials and Methods: Male mice were divided into 8 groups with 7 mice in each group: saline, ethanol, melatonin, memantin, MPTP, melatonin+MPTP, memantin+ MPTP, melatonin+ memantin+ MPTP. All of agents were injected intraperitoneally once a day for 14 days before beam traversal test. Dopaminergic neurons of the Substantia Nigra Pars compacta (SNPC) were determined by immunohistochemical and were counted.   Results: Melatonin improved notably movement dysfunction resulted of MPTP such as the number of errors, paces and the time of movement during behavioral test and also the counting of neurons of Substantia Nigra Pars Compacta. Memantin had a synergic effect on the most of improvements. However, the level of improvement and retrieval of signs was not as in saline and ethanol groups. Conclusion: Melatonin especially together with memantine is able to prevent some of the MPTP-induced dysfunctions. However, the protective effects were not enogh, probably because of the amount of dose and the time of injection. 3466 Infection Prevalence of Virulence Genes etA, etB in Community Acquired Methicillin-Resistant Staphylococcus aureus in Patients Referred to Teaching Hospital by PCR in Shahrekord, 2014 Abbasi Safiyeh Zamanzad Behnam Student Research Committee, Department of Microbiology, Shahrekord University of Medical Sciences, Shahrekord, Iran Department of Microbiology, Shahrekord University of Medical Sciences, Shahvekord, Iran 1 9 2015 18 6 42 50 27 01 2015 06 05 2015   Background: Colonization with MRSA is no longer limited to hospitalized patients or persons with predisposing risk factors and at present there are several strains of community-acquired MRSA (CA-MRSA). The purpose of this study is to determine etA and etB genes in isolated Staphylococcus aureus strains of clinical samples from teaching hospital in Shahrekord in 2014.   Materials and Methods: In this descriptive study, 220 clinical samples were collected from teaching hospitals in Shahrekord. The microbiologic characteristics of isolates were determined using microbiologyic standard methods. The MRSA detection was carried out on oxacillin agar medium. The detection of virulence genes etA and etB was used by PCR. Inducible resistance to clindamycin was tested by "D-test".   Results: In 220 isolates, 110 detected as S. aureus and 13 as MRSA. Inducible clindamycin resistance was seen in 4 (3.5%) of the isolates. The frequency of genes etA, etB in studied strains was 7.6 and 15.3, respectively. Also, Inducible resistance to clindamycin was seen in four isolates(2%). Conclusion: The results of this study confirme the presence of community- acquired strains in Shahrekord. The results of this study indicate the presence of genes etA and etB in the strains studied, transforming into the strains of Staphylococcus aureus resistant to methicillin obtained from hospital, the development and transfering these strains in the community. 3529 Infection The Correlation of Plasma Levels of Apelin-13 with Insulin Resistance Index and Plasma Leptin of Diabetic Male Rats after 8-Week Aerobic Exercise Kazemi Fahimeh Zahedi Asl Saleh Department of Physical Education and Sport Sciences, Shahid Beheshti University, Tehran, Iran Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 1 9 2015 18 6 51 60 23 02 2015 20 04 2015   Background: The physiological role of apelin, an adipokine secreted by adipose tissue , in insulin resistance and type 2 diabetes has been identified . The aim of this study was to determine t he correlation of plasma level s of apelin-13 with insulin resistance index (HOMA-IR) and plasma leptin of diabetic male rats after 8-week aerobic exercise .   Materials and Methods: Peresent study was an experimental study with animal model. Twenty eight diabetic male Wistar rats were divided into 3 groups: Non-diabetic (n=9), control diabetic (n=9) and trained diabetic (n=10). Type 2 diabetes was induced by intraperitoneal injection of nicotinamide and streptozotocin. The trained diabetic rat ran 8-week on treadmill progressively. After the training period, plasma levels of glucose, insulin, leptin and apelin-13 were measured and HOMA-IR was calculated. One-way analysis of variance (ANOVA) and Pearson’s correlation were used for analyzing data. p<0.05 was considered to be statistically significant.   Results: A significant decrease in plasma levels of glucose, insulin and leptin and HOMA-IR in trained diabetic vs control diabetic rats, a significant increase in plasma levels of apelin in trained diabetic vs non-diabetic and control diabetic rats and a significant negative correlation of plasma levels of apelin with HOMA-IR and plasma leptin in trained diabetic rats was observed.   Conclusion: In present study, 8-week aerobic training by improvement of insulin sensitivity (decrease of HOMA-IR and plasma leptin) increased plasma levels of apelin-13 in diabetic male rats. 3515 General Evaluation of Root Canal Morphology in the Mandibular First Premolars: A CBCT Study in An Iranian Population Kazemipoor Maryam Hajighasemi Afrooz Mirbeigi Sanam Department of Endodontics, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran Department of Oral & Maxillofacial Radiology, Shahid Sadoughi University of Medical Sciences and Health Servieces, Yazd, Iran 1 9 2015 18 6 61 68 18 02 2015 20 04 2015   Background: Mandibular premolars, because of high anatomic variations, are among the most difficult teeth for root canal treatment.The aim of this study is to compare and evaluate root canal morphology of mandibular first premolars between males and famales in an Iranian population applying CBCT imaging.   Materials and Methods: In this analytical-cross sectional study, 230 CBCT imaging belonged to 115 males and 115 females were evaluated in this study. Images were assessed in the three spatial planes and the difference in the number of canals and roots were compared and reported statistically. Data were analyzed using SPSS 17 software and Chi-square test.The significance level was set as p<0.05.   Results: In total, of 460 mandibular first premolars, 85.7% had one root, 14.3% had two roots, 63.9% had one canal and 36/1% had two canals. In comparison between two genders, in the males the prevalence of one root was 78.7%, two roots 21.3%, one canal 60% and two canals 40%. In the females, the prevalence of one root was recorded as 92.6%, two roots 7.4%, one canal 67.8% and two canals 32.2%. Conclusion: According to the results of this study the number of roots in the mandibular first premolars in males was statistically more than in females. 3339 Basic Sciences The Role of NO in Antispasmodic Activity of Parsley (Petroselinum crispum l.) Leaf Flavonoids Extract on KCl-Induced Contraction in Adult Male Rat's Ileum Govahi Azam Moazedi Ahmad Ali Seyyednejad Seyyed Mansor Pourmahdi Mehdi Department of Biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran Department of Biology, Shahid Chamran University, Ahwaz, Iran Department of Biology, Shahid Chamran University, Ahwaz, Iran Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid chamran, Ahwaz, Iran. 1 9 2015 18 6 69 77 07 12 2014 23 06 2015   Background: Due to the harmful side effects of chemical drugs, in recent decades, much attention has been returned to the use of medicinal plants. The flavonoids as plant secondary compounds are allocated to own an important part of this attention. Based on previous studies, some of the flavonoids are able to reduce contraction induced by KCl, acetylcholine, etc in rat ileum. Therefore, in this study, the effect of the flavonoid extract of parsley leaves on smooth muscle contraction of the ileum and the role of Nitric oxide (NO) has been studied.   Materials and Methods: Distal segment of Wistar rat ileum was separated and contractions under one gram tension and at 37°C, in an organ bath containing Tyrode solution was recorded by isotonic method. Rats were divided into 2 groups with 7 in each group. After placing the tissue exposed to L-NAME 100&muM (Sigma, America) as an inhibitor of NO, the antispasmodic effect of cumulative concentrations of the flavonoid extract was investigated   Results: The extract cumulative concentrations reduced ileum contraction induced by KCl in a dose-dependent manner. Also, the antispasmoide effect of flavonoids extract on ileum contractions (only 16 mg/ml) was affected by L-NAME. Conclusion: This study showed that the extract relaxed rat's ileum by the NO system at higher concentrations. 3364 Pediatrics Comparing the Effect of Probiotics and Polyethylene Glycol in Treatment of Children with Functional Constipation Hashemi Mojtaba Javaheri Javad Habibi Marjan yousefi chaijan Parsa Naziri Mahdyieh Department of pediatrics, Arak University of Medical sciences, Arak, Iran Department of pediatrics, Arak University of Medical sciences, Arak, Iran Department of pediatrics, Arak University of Medical sciences, Arak, Iran Department of pediatrics, Arak University of Medical Sciences, Arak, Iran Depatrment of Base Sciences, Arak University of Medical sciences, Arak, Iran 1 9 2015 18 6 78 85 21 12 2014 20 04 2015   Background: The prevalence of constipation in childhood is 0.7% to 29.6%. Inorganic causes are considered as the most common cause of constipation in children. Polyethylene glycol (PEG) is the most effective and safest laxatives which is usable in children in the long-term. This study was conducted to evaluate and compare the effect of PEG treatment and probiotic bacilluscoagulans and bifidobacterium and probiotic to enhance the growth of probiotics.   Materials and Methods: In this double blind clinical trial study(interventional study), 120 children aged 2-16 years with chronic functional constipation enrolled the study based on ROME III criteria. The bowels became empty by using liquid paraffin. Then, children were randomly divided into three groups of polyethylene glycol+placebo and Probiotics+placebo and polyethylene glycol+probiotics recipients. After 6 weeks of treatment, treatment success rates between the three groups were compared before and after the study Data analyzed by SPSS 20 software.   Results: The results of the study conducted on the three groups for treatment of constipation showed that the effect of polyethylene glycol and probiotic on treatment was considerable before and after treatment but the levels of effect was similar in the comparison of groups. The simultaneous use of two drugs was more effective than a single drug. Conclusion: Overall, the results of our study showed that concurrent use of polyethylene glycol and probiotics is much more effective in the treatment of constipation.