46 1735-5338 Arak University of Medical Sciences 3802 General The Effect of Selective Aerobic Training on Serum Irisin Levels and Insulin Resistance Index in Women with Type 2 Diabetes Aghamohammadi Mozhgan b Habibi Abdolhamid c Ranjbar Roohollah d b Facalty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran. c Department of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran. d Department of Exercise physiology, Shahid chamran University of Ahvaz, Ahvaz, Iran. 1 2 2016 18 11 1 9 26 06 2015 12 09 2015 Background: Irisin, a recently identified myokine that seems had important role in hemostasis regulatation and improving insulin resistance. The effect of exercise training on serum irisin levels in type 2 diabates (T2D) is controversial. This study aims to investigate the effect of 6 weeks selective aerobic training on serum irisn levels and insulin resistance in women with T2D. Materials and Methods: This research is a semi experimental study with pre-and post-test design. Research subjects were 20 women with T2D (mean age 47.4±5.6 years and BMI 29.92±3.42). Subjects were randomly divided into 2 groups: exercise group (n=12) and control group (n=8). Training protocol performed in 6 weeks (4 times a weeks, 40-65 min per session) and main training intencity and time were increased per week (50%-80% Hrmax). Main aerobic training was performed in 6 blocks, each block consisted of 32. Blood sample was collected before the and after end of training protocol. Results: The results showed significant decrease in blood glucose, insulin and insulin resistance index and significant increase in serum irisin levels in exercise group compared with the control group (p≤0.05), but there is no significant correlation between serum irisin levels and insulin resistance. Conclusion: It seems that aerobic training can have considerable impact on serum irisin levels, fasting blood glucos and insulin resistance index in women with T2D.
3942 General Measuring the Constructs of Planned Behavior Theory Regarding the Behaviors Preventing of Junk Food Consumption in Elementary Students in Arak in 2015 Barati Faezeh e Shamsi Mohsen f Khorsandi Mahboobeh g Ranjbaran Mehdi h e Department of Health Education, Arak University of Medical Sciences, Arak, Iran. f Department of Health Education, Arak University of Medical Sciences, Arak, Iran. g Department of Health Education, Arak University of Medical Sciences, Arak, Iran. h Department of Epidemiology, Arak University of Medical Sciences, Arak, Iran. 1 2 2016 18 11 10 18 01 09 2015 02 11 2015 Background: Today, the consumption of junk food as a snack among children, especially in elementary school students is on the rise. This research has measured Theory of Planned Behavior constructs in this field. Materials and Methods: This is a cross-sectional and analytical study that was carried out on 217 primary school students in Arak city in 2015. Students were selected according to cluster sampling method. Data collection instrument was a researcher-developed questionnaire consisting of theory of planned behavior constructs such as attitudes, subjective norms, perceived behavioral control, behavioral intention and performance. Reliability and validity of the questionnaire was studied before the study. Multivariate linear regression model was used to determine the most important predictive constructs of students behavior. Results: In this study, the mean and standard deviation score of attitude (75.41±12.42), subjective norm (78.25±11.20), perceived behavioral control (63.69±17.06), behavicral intention (76.26±15.44), and performance (48.84±24.44) were observed. Based on the results, perceived behavioral control could predict 23 percent change in behavior. There was a direct correlation between the score of constructs and performance(p<0.05). Moreover, there were not any significant differences(p>0.05) between students nutrition and age(r=0.019), weight (r=0.07), height (r=0.12) and BMI (r=0.07). Conclusion: The results of this study suggested that positive attitude and self-esteem must taken into account to control students diet, in the design of interventions to prevent consuming junk food. 3684 Obstetrics & Gynocology Analysis of ESR1 rs104893956 Polymorphism with Infertility in Guilanian Women Jodeiry Sheyda i Vaziri Hamid Reza j Zahiri Ziba k i Department of Biology, University of Guilan, Rasht, Iran. j PhD in Cell and Molecular Biology, Department of Biology, University of Guilan, Rasht, Iran. k Department of Gynaecology, Guilan university of Medical Sciences, Rasht, Iran. 1 2 2016 18 11 19 26 12 05 2015 12 09 2015 Background: Infertility is a multifactorial disorder with genetic and non-genomic factors. It is estimated that female infertility factors accounts for more than 40%. Estrogen is one of the effective hormons  in fertility. Its crucial actions on target tissues are mediated via binding to estrogen receptors(ESR). The ESR1 gene is located on chromosome 6q25.1 and encodes α estrogen receptor. The aim of this study was to analysis of ESR1 rs104893956 polymorphism in female infertility. Materials and Methods: In this case-control study, of 60 infertiles and 55 healthy controls, blood samples were attained. After the extraction of genomic DNA from peripheral blood leukocytes, Allele Specific-PCR (AS-PCR) method was applied for determining the codon polymorphism. Statistical analysis was performed using the MedCalc software (Version 12.1). Results: The frecuency of T allele was significantly higher in patients (58%) than the controls (44%). There was higher frequency of TT genotype of the polymorphism in patients (18.33%) compared with controls (1.8%). Our findings revealed that the patients carrying the TT genotype had a significant increased risk of infertility. Conclusion: The results of this study suggests that ESR1 rs104893956 polymorphism may affect the increased susceptibility to female infertility in Guilan province. The results may be different in other genetic pools or large-studied population. 3699 Basic Sciences Analysis of GPX-1 Pro198 Leu Gene Polymorphism in Patients with Diabetic Retinopathy in Rasht Zamani Sonya l Mashayekhi Farhad m Salehi Zivar n Abbasi Nasim o l Department of Biology, University of Guilan, Rasht, Iran m Department of Biology, University of Guilan, Rasht, Iran n Department of Biology, University of Guilan, Rasht, Iran o Department of Biology, University of Guilan, Rasht, Iran 1 2 2016 18 11 27 33 19 05 2015 05 12 2015 Background: Diabetic retinopathy (DR) is the complication of diabetes mellitus (DM) and causes blindness among adults. Chronic extra cellular hyperglycemia in diabetes stimulates reaction oxygen species ROS production and increases oxidative stress. GPX-1 that was coded by GPX-1 gene is a key enzyme in protecting vessels against oxidative stress. The aim of this study was to evaluate the association of GPX-1 gene Pro 198 Leu polymorphism in patients with diabetic retinopathy. Materials and Methods: In this case-control study, 160 blood samples of participants including 80 patients with diabetic retinopathy and 80 healthy individual were tested. Genotyping of GPX-1 gene was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) by ApaI enzyme. Data analysis was performed using MedCalc (12.1) program. Results: The genotype frequencies of the GPX-1 in DR patients for Leu/Leu, Leu/Pro, Pro/Pro were 10%, 62.5% and 27.5%, respectively, while for the control groups were 10%, 70% and 20%, respectively.In ohter words, Ile/Pro heterozygote was the most frequent genotype in patients and controls. According to the results of this study, there was not significant difference between patients with diabetic retinopathy and controls(p=0.52). Conclusion: It is concluded that GPX-1 gene Pro 198 Leu polymorphism is not associated with DR. Further research is required to clarify the role of GPX-1 gene in DR in Rasht population along higher sample size. 3893 Basic Sciences Identification of the Chemical Composition of Essential Oil and Evaluation of Antibacterial Activity of Methanolic Extracts from the Aerial Parts of Ballota Platyloma Rech. f. Seyedalipour Begher p Hasani Ali Ebrahimzadeh Mohammad Ali Mohseni Mojtaba p Department of Cellular and Molecular Biology, University of Mazandaran, Babolsar, Iran Department of Biochemistry, Sciences and Research Branch, Islamic Azad University, Sanandaj, Iran Department of Pharmacochemistry, Mazandaran University of Medical Sciences, Sari, Iran Department of Cellular and Molecular Biology, University of Mazandaran, Babolsar, Iran 1 2 2016 18 11 34 43 03 08 2015 29 09 2015 Background: Lamiaceae family has about 200 genuses and 4000 species. The majority of species of the family have essence that was used for nutritional, cosmetic and pharmaceutical industry. The aim of this study was to investigate the chemical composition of essential oil from aerial parts of Bollota platyloma  and antibacterial activity of it. Materials and Methods: In this experimental study, study Ballota platyloma, belonging to lamiaceae family and entomic of iran, was collected from Versk region in Mazandaran provinc. Essential oil from aerial parts was obtained by hydro distillation method by using Clevenger apparatus. GC/MS analysis of essential oil was carried out to identify major volatile constituents. Extraction was prepared by maceration method. Antibacterial activity of methanolic extracts against Pseudomonas aeruginosa, Bacillus subtilis, Stapylococcus aureus, Escherichia coli was investigated by disc diffusion method and minimal inhibitory concentration(MIC) method. Results: Results showed that GC/MS analysis of the essential oil confirms the presence of 24 constituents in Ballota platyloma. The main constituents were Hexadecanoic Acid (40.03%), Germacrene D (26.6%) and Beta Caryophylene (4.76%). The results indicated that methanolic extract of Ballota platyloma possessed antibacterial activity. Among the aforementioned bacteria, the highest antibacterial activity was seen against S. aureus and the lowest activity against P. aeruginosa. Conclusion: The findings of the present study showed that the major component of oil essential was germacrene-D and methanolic extract from the aerial parts of Ballota platyloma Rech. f. possess anti-bacterial effects. Thus, in order to find the underlying mechanism of this activity, further research should be carried out. 3922 Basic Sciences Determining the Agr Gene Variety (Accessory Gene Regulator) in Susceptible and Methicillin-Resistant Staphylococcus Aureus Strains in Clinical Samples and Carriers Employed in Remedial Centers Arabestani Mohammad Reza Abdoli Kahrizi Mohammad PhD in Medical Bacteriology, Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran 1 2 2016 18 11 44 53 18 08 2015 07 10 2015 Background: Agr systems, is responsible for control and coordination in production of virulence factors, exotoxins secretory and hemolysins in Staphylococcus aureus. The aim of this study was to determine and identify the frequency of agr genes in susceptible and methicillin-resistant Staphylococcus aureus strains in clinical samples and carriers employed in remedial centers. Materials and Methods: This descriptive study was done among a total of 200 strains of Staphylococcus aureus isolated from clinical samples and healthy carriers in Hamadan. Antibiotic susceptibility pattern of all isolates was determined by disk diffusion methods. After DNA extraction, the presence of mecA and agr genes was investigated using PCR. SPSS software package version 20 was used to perform statistical tests. Results: All 200 Staphylococcus aureus strains were susceprible to vancomycin. The prevalence of mecA was 50%. The PCR results showed that agrA was the most perevalent gene followed by the agrC in all isotated Staphylococcus aureus strains. None of the isolates harbored the agrB and agrD gene. Conclusion: Pathogenesis of Staphylococcus is dependent on some proteins other superficial or excreted which under controlling of agr system. In the present study, the feequency of agrA gene in the methicillin-resistant strains, methicillin-sensitive strains isolated from clinical samples and carriers employed in remedial centers was higher than the other agr types. Therefore, presumably, agrA gene plays an important role Staphylococcal infections. 3781 Orology Effect of the Alcoholic Extract of Portulaca Oleracea on Renal Ischemia/Reperfusion Injury in Rats Askaripour Majid Fatemi Tabatabaei Syed Reza NajafzadehVarzi Hossein Hosseini Foruzan Department of Physiology, Shahid Chamran University, Ahvaz, Iran Department of Physiology, Shahid Chamran University, Ahvaz, Iran Department of Pharmacology, Shahid Chamran University, Ahvaz, Iran. Department of Physiology, Kurdistan University of Medical Sciences, Sanandaj, Iran 1 2 2016 18 11 54 62 19 06 2015 05 10 2015 Background: Renal injury following ischemia - reperfusion (I/R) is still an unavoidable problem in many remedial and medical situations. Portulaca oleracea (PO) has been known for its anti-oxidative effects. Then, the present study aimed to investigate the effect of ethanolic extract of PO (EEPO) on the renal function and antioxidant status after induction of I/R injury in the rat kidney. Materials and Methods: A total of 30 rats (Wistar) were divided into five groups (n = 6 each). Sham group: underwent laparotomy without I/R, EEPO group: EEPO administered 300 mg/kg then was operated like sham, I/R group: was underwent renal ischemia/reperfusion only, EEPO150+ I/R and AEPO300+ I/R groups: were administered PO 150 and 300 mg/kg then underwent I/R operation. PO extract was administered for 5 days in the relevant groups by gavage. Serum urea and creatinine (Scr), the level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and total antioxidant activity (TAA) were determined. Data were analyzed by ANOVA and post hoc LSD test. P values of 0.05 or less were considered statistically significant. Results: Induction of I/R and pretreatment with PO extract, increased the level of superoxide dismutase (SOD) in comparison with sham group (p<0.05, p<0.001). There were no significant differences in the levels of MDA, GSH and TAA among different groups. On the other hand, the Scr and serum urea of the I/R and treated groups were elevated compared to the sham group (p<0.001). Conclusion: Ethanolic extract of PO did not strongly affect the renal antioxidant status and could not prevent the renal injury following I/R. 3548 General Relationship between Job Satisfaction, Death anxiety and Sleep Quality of Nurses in the Hospitals of Arak University of Medical Sciences Kasraie Ehsan Rafeie Mohammad Mousavipour Saeid Arak University of Medical Seiences, Arak, Iran. Department of Health and Community Medicine, Arak University of Medical Sciences, Arak, Iran. Department of Psychology and Educational Sciences, Arak University, Arak, Iran. 1 2 2016 18 11 63 74 02 03 2015 29 09 2015 Background: Today, occupational health of nurses in the health care system is important. This study aimed to investigate the relationship between death anxiety, sleep quality and job satisfaction of nurses working in Arak university of medical sciences hospitals in 2015. Materials and Methods: The research method was descriptive, correlational and cross-sectional. The population included nurses in public hospitals affiliated with Arak university of medical sciences. The samples included 309 nurses that were simply and randomly selected. Data were collected with Herzberg job satisfaction, Pittsburgh sleep quality questionnaires and death anxiety scale. Data were analyzed using descriptive and inferential statistics. Results: The results showed that the relationship between job satisfaction with sleep quality of nurses was significant, but there was not a significant relationship between job satisfaction and quality of sleep with death anxiety of nurses. In addition, there was a significant telationship between diffevent parts of hospitals in job satisfaction and death onxiety, although this difference was not significant in quality of sleep. Job satisfaction was the only factor that was significant in the group with the possibility of difficult working conditions. Conclusion: According to the research findings, it seems that paying attention to sleep quality sleep is important to improve nurses satisfaction. Job satisfaction is also influenced by the hard conditions of work and requires more attention to a group of nurses who are working in such a situation. Also, a higher death anxiety than usual in nurses requires more study and reflection. 3602 Anesthesia An Assessment on Post Cesarean Pain Relief in Preeclampsia Parturient who Received Magnesium Sulfate Mansourghanaei Mandana Haryalchi Katayoun Asgari Seyed Alaedin Salamat Fatemeh Zoghi Mahdieh Department of Obstetric and Gynecology, Reproductive Health Research Center, Gulian University of Medical Sciences, Gulian, Iran. Department of Anesthesia, Guilan University of Medical Science, Guilan, Iran. Department of Urology, Guilan University of Medical Science, Guilan, Iran. Research and Technology Deputy, Guilan University of Medical Science, Guilan, Iran. Guilan University of Medical Science, Guilan, Iran. 1 2 2016 18 11 75 82 05 04 2015 17 10 2015 Background: MgSo4 (magnesium sulfate) is the first therapeutic line for preeclampsia. Recently, there have been many debates on pain relief property on MgSo4. The purpose of this study is to evaluate the effect of MgSo4 on pain relief after cesarean in preeclampsia parturient Materials and Methods: In this analytic and cross-sectional clinical trial study, 88 pregnant wowen with mild preeclampsia who received MgSo4 (14g loading dose and 5 g/4h maintenance dose) (n=88, P group), were compared with 88 normal pregnant women(n=88, N group), according to the duration of paim relief and the amount of diclofenac suppository consumption after cesarean section from March 2013 to October 2014. After cesarean section, the assessment of pain relief performed with NRS (numerical rating scale), and diclofenac consumption. Results: P group had a longer analgesic duration than N group. NRS in P group was significantly longer than N group(6.89 ±3.34 vs. 3.55± 2.13 hr, p=0.0001). The dose of diclofenac suppository in P group was significantly lower than N group (225mg±147 vs. 365.9±92mg, p<0.0001). Conclusion: Post cesarean pain in preeclamptic group was significantly lower than non-preeclamptic group. This finding is probabley because of MgSo4 administration in preeclamptic group. 3870 General Effects of Continuous Training Intensity on Amyloid Beta1-42(Aβ1-42) Levels in Hippocampus of Homocysteine-Induced Alzheimer's Model Rats Yaghoubi Ali Saghebjoo Marziyeh Fallah Mohammadi Zia Hedayati Mehdi Hajizadeh Moghaddam Akbar Department of Physical Education, University of Birjand, Birjand, Iran. Department of Physical Education, University of Birjand, Birjand, Iran. Department of Exercise Physiology, University of Mazandaran, Babolsar, Iran. Cellular and Molecular Research Center, Institute for Endocrine Sciences and Metabolism, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Biology, University of Mazandaran, Babolsar, Iran. 1 2 2016 18 11 83 93 21 07 2015 07 10 2015 Background: The Amyloid beta (Aβ) level increases in the brain of patients with Alzheimer's disease. The present study aimed to investigate the effects of eight weeks continuous training with low and high intensities on Aβ1-42 levels in hippocampus of Alzheimer model rats. Materials and Methods: Fifty male Wistar rats (12 weeks old and mean weight 219.82±13.10 g) were divided into five groups including: healthy control, Alzheimer’s control, Alzheimer's low-intensity training, Alzheimer's high-intensity training and sham. To induce Alzheimer's disease, homocysteine is infused into the rats cerebroventricular (dose of 0.6M). Low intensity groups trained with 20m/min (50-55% VO2max) and high-intensity groups trained with 27m/min (75-80% VO2max), 60min/day, and five days per week on the treadmill. For data analysis, one-way ANOVA and post hoc Tukey test were performed (p<0.05). Results: The Aβ1-42 levels in hippocampus of Alzheimer's control group was significantly higher than healthy control group (p=0.001) and in training groups with both low and high intensity was significantly lower than Alzheimer's control group (p=0.02). But no significant differences were found between two intensity (p=0.99). Conclusion: It seems that continuous exercise training, through reducing the level of Aβ1-42 in hippocampus, can be useful for Alzheimer’s disease model rats and continuous training can be studied as a complementary therapy in Alzheimer's disease.