2024-03-29T13:53:00+03:30 http://jams.arakmu.ac.ir/browse.php?mag_id=64&slc_lang=fa&sid=1
64-2746 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Evaluating the Aluminum Induced Oxidative Stress in Erythrocyte and Plasma of Operating Room Personnel Hadi Ansari Hadipour ansary@arakmu.ac.ir Ali Molaei Nezamabadi Background: Our Previous studics hane been shown that iron ions produce oxygen free radical reaction products in the erythrocytes. The present study is evaluating the oxidative effects of aluminum in erythrocytes and plasma proteins and decline of antioxidant power of plasma in operating room personnel. Materials and Methods: Blood obtained from operating room personnel. Erythrocytes and plasma were incubated aerobically in aluminum containing metal catalyzed oxidation (MCO) system. Total antioxidant capacity of plasma was estimated by FRAP assay (ferric reducing ability of plasma), plasma protein oxidation by carbonyl assay and structural changes in Hb were followed by spectrophotometric analysis and assessment of oxy-Hb, met-Hb and hemichrome. Results: There was a significant relationship between optical density of Hb in 340 and 420 nm and between oxy-Hb and optical density of Hb in 420 nm(p<0.05) On the other hand, 68 to 56% decrease in total antioxidant power of plasma in control group and operating room personnel, results oxidation in plasma protein and carbonyl concentration was increased three fold. Conclusion: These findings may be helpful in evaluating the antioxidant status and structural changes in Hb and plasma proteins in people who are exposed to aluminum especially operating room personnel. Aluminum Erythrocyte Ferric reducing ability of plasma Hemoglobin 2014 6 01 1 9 http://jams.arakmu.ac.ir/article-1-2746-en.pdf
64-2786 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Effect of Afrodit on Sexual Satisfaction in Menopausal Women Simin Taavoni taavoni@yahoo.com Neda Nazem ekbatani neda40012@gmail.com Hamid Haghani haghani511@yahoo.com Background: One of the most common problems in Menopausal women is reduce of sexual satisfaction, which can affect their quality of life. The aim of the present study was the effect of Afrodit on sexual satisfaction in postmenopausal women. Materials and Methods: In the triple blind clinical trial with the placebo group participated 80 healthy females 50-60 years that were referred to health centers affiliated to Tehran University of Medical Sciences. Participants were randomly assigned to two groups, Afrodit groups and placebo capsules for a month. Used tool in this study was Sabatsbrg. Part of which was to assess sexual satisfaction. Results: After a month intervention, there is significant difference in the mean score for sexual satisfaction between two groups (p=0.01), Sexual satisfaction compared with pre-menopausal in two groups were compared. T-test results showed no significant difference. (p=0.16). Conclusion: The results show Afrodit improves the sexual satisfaction in menopausal women. So it is essential that health providers are familiar with this herb supplement. Menopause Sexual satisfaction Sexual function sabatsbrg measure Afrodit 2014 6 01 10 18 http://jams.arakmu.ac.ir/article-1-2786-en.pdf
64-2783 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Investigation of HLA-DRB and DQB in Multiple Sclerosis Patients by Single Specific Primer-Polymerase Chain Raction (PCR- SSP) Mana Shojapour Ghasem Mosayebi ghasemmosayebi@arakmu.ac.ir Fardin Faraji Keyvan Faraji Ali Ghazavi Background: Multiple sclerosis (MS) is an autoimmune disorder with unknown etiology. Genetic and environmental factors associated with MS susceptibility. Genetic studies show an important role for human leukocyte antigens (HLA) and susceptibility to autoimmune diseases such as MS. There is controversy between the association of HLA alleles with MS susceptibility in various studies. However, with consider the high incidence of MS in Iranian population and limit information about association of HLA and MS, we analyzed HLA alleles in MS patients. Materials and Methods: In this case-control study, 60 MS patients and 40 normal individuals with the same ethic background and geographic area were analyzed for HLA-DRB and DQB alleles by single specific primer-polymerase chain reaction (SSP-PCR) method. Results: HLA-DRB1*03 and DQB1*02 alleles frequencies in MS patients were greater than healthy controls. There was no significant difference in frequency of other HLA-DR alleles between the MS patients and normal individuals. Conclusion: DRB1*03 and DQB1*02 alleles confer increased susceptibility to MS in this population. However, to determine the role of HLA in Iranian MS patients, more studies are needed. Human Leukocyte Antigens Multiple sclerosis Polymerase chain reactions 2014 6 01 19 25 http://jams.arakmu.ac.ir/article-1-2783-en.pdf
64-2770 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 The Effect of Eight Week of High Intensity Interval Training on Expression of Mir-210 and EphrinA3 Mrna in Soleus Muscle Healthy Male Rats Ahad Shafiee A.shafiee@ut.ac.ir Mohamadreza kordi Abbasali Gaeini Masoud Soleimani Amin Nekouei Vahid Hadidi Background: Mir-210 is proangiogenic microRNAis endothelial cells. This microRNA, causes the repression of some genes and proteins target so cause angiogenesis process. The purpose of this study was to determine the effect of a High Intensity Interval Training (HIIT) on Mir-210 and EphrinA3 receptor genes expression in soleus muscles of male rats. Materials and Methods: In this experimental study, Twelve Wistar male rats(ageof eightweeks, average weight of 210.5±9.77)were randomly divided into exercise(n=6)and control (n=6) groups. High Intensity Interval Training was formed five days a week for eight weeks to taly including three Intervals (four minutes with an intensityof 90 to 100%VO2max and two minutes with an intensityof 50 to 60%VO2max).24 hours after exercise protocol, the rats were dissected and separated soleusmuscle. Mir-210 and EphrinA3receptor genes expression was performed by Real Time-PCRtechnique. Mir-210 and EphrinA3receptor genes expression were calculated by using the2∆∆CT and in dependentt-test to determine the significance of variables. Results: Results showed that HIIT there had no significant effects on Mir-210 gene expression (p=0.16) Whe ars EphrinA3 gene expression in the exercise group was statistically significant (p=0.000). Conclusion: It seems that a non-significant increase of Mir-210 and reduce in EphrinA3 gene expres sion, causes proangiogenic Operation ofendothelial cells and an increase in VO2max of rats following eight weeks of HIIT performance can be due to increased angiogenesis process. Angiogenesis EphrinA3receptor High intensity interval training MicroRNA 2014 6 01 26 34 http://jams.arakmu.ac.ir/article-1-2770-en.pdf
64-2713 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 The Comparison of Antimicrobial Effects of Chevil (Ferulago Angulata) Extract with a Variety of Common Therapeutic Antibiotics In Vitro Farideh Tabatabaei Yazdi farideh_tabatabaee@yahoo.com Behrooz Alizade Behbahani Maryam Heidari Sureshjani Background: The overuse of therapeutic antibiotics results in the drug resistance. The excessive use of antibiotics causes the mutations in the microorganisms and the emergence of new microorganisms which are resistant against the common antibiotics. With regard to limitations and known side effects of antibiotics, the exploring of antimicrobial compounds seems necessary. The purpose of this study is to evaluate the antimicrobial effects of aqueous and ethanolic extracts of Chevil against Staphylococcus epidermidis PTCC 1435, Yersinia enterocolitica PTCC 1221 and Enterobacter aeruginosa PTCC 1151 and to compare them with the common therapeutic antibiotics. Materials and Methods: In this experimental study, after collecting plants from the highlands of Chaharmahal va Bakhtiari province, the extraction was carried out by the maceration method. To evaluate the antimicrobial activity, Disc diffusion test with Kirby-Bauer method was used. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using the dilution method. Results: The highest inhibition zone diameter in 40 mg/ml was related to Staphylococcus epidermidis and the minimum diameter in this concentration was related to Gram-negative bacteria, Enterobacter aeruginosa. MIC of aqueous and ethanolic extracts for Enterobacter aeruginosa 64 and 32 mg/ml and MBC of ethanolic and aqueous extracts of Enterobacter aeruginosa were 128 and 64 mg/ml respectively. Conclusion: The ethanolic extract of the Chevil compared with the common therapeutic antibiotics had more inhibitory effect on studied bacteria. Furthermore, Chevil extracts showed greater inhibitory effect on Gram-positive bacteria in comparison with Gram-negative bacteria. Antibiotic Drug resistance Ferulago angulata Inhibition zone diameter 2014 6 01 35 46 http://jams.arakmu.ac.ir/article-1-2713-en.pdf
64-2780 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Improving the Quality of Life in Tuberculosis Patients with N–Acetylcysteine Aliasghar Farazi farazialiasghar@yahoo.com Masoomeh Sofian ma_sofian@yahoo.com Mansoureh Jabbariasl jabbariasl@yahoo.com Background: The importance of maintaining and enhancing the quality of life in (tuberculosis TB) patients is essential. The purpose of this study was to use N - acetylcysteine to improve the quality of life in patients with tuberculosis. Materials and Methods: This study is a double-blind clinical trial that performed on 88 patients on anti-TB treatment that aged over 50 years in the central province of Iran within 15 months. Data collection tools included a demographic questionnaire and the Short Form Quality of life (SF12). Data were analyzed using SPSS software and t-test, Chi-square, Mann-Whitney, ANOVA and Pearson correlation analysis were used. p less than 0.05 was considered significant. Results: This study showed that the two groups before the intervention in different features were not significantly different, but in quality of life at the end of the first month. The intervention group compared with the control group in physical subscale score (p=0.0068) and psychological subscale scores (p=0.0284) and quality of life score (p=0.0112) were better. Also, our study showed that age of patients (p=0.0331), duration of disease (p=0.0416), and serum CRP mean (p= 0.0234) and type of tuberculosis (p=0.0372) were significantly related to total score of quality of life at the end of the first month in the intervention group. Conclusion: According to this survey results we hoped to combine the use of adjuvant therapy with N-acetylcysteine and standard tuberculosis treatment improves quality of life and increase life expectancy of patients. N-acetylcysteine Quality of life Tuberculosis 2014 6 01 47 55 http://jams.arakmu.ac.ir/article-1-2780-en.pdf
64-2404 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Evaluation of the Efficiency of Electrolysis Process with Continuous Flow in the Disinfection of Water Contaminated with Fecal Coliform Mohammad Reza Massoudinejad Ashraf Mazaheri Tehrani mazaheri@kaums.ac.ir Farshid Ghanbari Simindokht Mirshafian Background: The conventional methods of water disinfection are chemical process, ozonation, UV radiation, membrane processes and etc. In recent years, electrolysis method has been considered that is a green process with high efficiency and not by-products. The aim of study is evaluation of efficiency of the electrolysis process with continuous flow in the disinfection of water contaminated with fecal coliform. Materials and Methods: This study is a Descriptive - analytical study. The samples are prepared in three groups by adding domestic wastewater, manure and E.Coli colonies to distilled water. The prepared samples were introduced to continuous electrochemical reactor. The removal efficiency of electrolysis process was investigated in different conditions which include electrode material (copper and graphite compressed), reaction time (40, 50, 60 and 70 minutes), voltage 48V, distance of electrode 5 cm and the initial pH 7. Results: The results indicate that removal efficiency depends on source of pollutant, reaction time, and type of electrode. Also the optimal efficiency for E.Coli colonies achieved in electrolysis instrument was as follows: electrode material = copper electrode, distance of electrode=5cm, applied voltage= 48V and reaction time=70 minute. Under these conditions removal efficiency was obtained 99%. No significant changes in pH, TDS and EC in different times and coliform bacteria were not created by changing the source. Conclusion: According to the results of this study, using the electrolysis process with continuous flow, as a convenient method with high performance and environmentally, suggest for the disin fecting the water contaminated with fecal Coliform. Coliform Disinfection Electrolysis Escherichia coli Water 2014 6 01 56 64 http://jams.arakmu.ac.ir/article-1-2404-en.pdf
64-2756 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 In Vitro Evaluation of Loading and Release Rate of Two Enteric Coats for Nucleic Acid Delivery Sedigheh Momenzadeh Abdorrahim Sadeghi sadeghi@arakmu.ac.ir Nasimeh Vatan Doust Rsoul Salehi r-saleh@med.mui.ac.ir Background: Gene therapy is a recent promising treatment that effective gene transfer is considered as its most important step. Furthermore noninvasive method of transfer will be important to, when gene therapy is supposed to be applied. Administration of drugs in oral rout is more appreciated by patients. Loading and release rates are very important in targeting and effectiveness of transfer in all different methods that have been used for oral drug transfer. Here, we have studied packing of gene particles into two different enteric coats and compared these two coats in loading of entrapped materials and there release rate in vitro. Materials and Methods: First, DNA was mixed with chitosan by coacervation technique and resulted polyplexes were coated using solvent evaporation technique. FTIR and two different pHs, less and more but near eudragit pKa, were used to evaluate formation of particles and their behavior. Results: Formed particles have similar stability in low pH and their differences are trivial. Eudragit L100 release rate is really slower than L100-55 and gradual. Eudragit L100 shows better ability in loading rate. Conclusion: According to two formed particles;#39 behavior, eudragit L100-55 might be used in oral gene transfer targeting of initial part of small intestine and eudragit L100 might be used for wider surface of small intestine, from the initial to the end part, and colon. Chitosan Enteric coat Eudragit Oral delivery 2014 6 01 65 72 http://jams.arakmu.ac.ir/article-1-2756-en.pdf
64-2777 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Protective Effect of Curcumin on Testis Histopathology in Sodium Arsenite-Treated Adult Mice Hamid Reza Momeni h-momeni@araku.ac.ir Malek Soleimani Mehranjani m-soleimani@araku.ac.ir Najmeh Eskandari n-eskandari@araku.ac.ir Vahid Hemayatkhah Jahromi Vahid hemayatkhah@gmail.com Background: Arsenic as an environmental contaminant induces male infertility. Curcumin with potent antioxidant property is able to restrict oxidative stress. The aim of this study was to investigate the effect of curcumin on testis tissue and sperm count in adult mice treated with sodium arsenite. Materials and Methods: In an experimental study, animals were divided into four groups: control, sodium arsenite (5 mg/kg), curcumin (100 mg/kg) and curcumin+sodium arsenite. Treatments were performed by intraperitoneal injection for five weeks. After treatment period, body weight was recorded. Left testis was dissected, weighed and used for the histopathological study of seminiferous tubules. Left cauda epididymis was also used to count sperm number. Results: Mice treated with sodium arsenite showed a significant decrease in the sperm count, the diameter of seminiferous tubules and a significant increase in the lumen diameter of tubules compared to control group. In curcumin+sodium arsenite group, curcumin significantly reversed the adverse effects of sodium arsenite on testis and sperm count. Whereas, the treated mice showed no significant difference in body and testis weight as well as morphology and nuclear diameter of spermatogonia between four groups. Conclusion: Curcumin is able to compensate the toxic effect of sodium arsenite on sperm count and testis in adult mouse. Adult mouse Curcumin Sodium arsenite Sperm count Testis 2014 6 01 73 81 http://jams.arakmu.ac.ir/article-1-2777-en.pdf
64-2621 2024-03-29 10.1002
Journal of Arak University of Medical Sciences J Arak Uni Med Sci 1735-5338 2008-644X 2014 17 3 Rare Lower Limb hydatid Cysts Presenting as Mass Mohamad Reza Imani imani_mreza53@yahoo.com Adnan Tiz Maghz adnan_ti@yahoo.com Mir Ahmad Salmasi Background: Hydatid disease is a parasitic infestation by a tapeworm of the genus Echinococcus. Man is an intermediate or accidental host of this parasite, and is infested by consuming contaminated plants or vegetables, or by contact with dogs. The larvae travel through the gut wall and 60-75% are filtered by the liver, and a few travel directly to the lungs to produce a hydatid cyst there. Growth of hydatid cysts has been reported in the spleen, muscles, bones, and brain. Hydatid cyst embolism to peripheral limb arteries is extremely rare. Case: A 41-year-old gravided woman presented with sudden pain, erythema and warming of the olden infra-inguinal mass which existed from 1 year ago. The notable findings at physical examination included 40*20 mm diameter soft mass with warming and erythema in 4 cm below inguinal ligament of right lower limb. She didn’t mentioned any previous history hydatid cyst. Intraoperatively, Percutaneous aspiration of the mass was performed which demonstrate white turbid fluid. after the surgical incision we saw the white colored subcutaneous hydatid cyst and completely remove it and send it to pathological examination which confirmed hydatid cyst diagnosis. further liver ultrasound and chest radiography hasn’t shown any evidence of liver or lung involvement. Conclusion: Cystic echinococcosis may affect all organs, but mostly settles in the liver and lungs. We do not have data on the means by which the larvae of echinococcosis reach the inguinal area without liver and lung involvement. Considering the anatomy of the groin and its lymphatic tissue, we can make an assumption about how the hydatid cysts settle in the groin space. Although it occurs rarely, larvae in the gastrointestinal tract may pass to the lymphatic circulation, and then settle in the groin space through the rich lymphatic flow of inguinal area. Hydatid cyst Echinococcosis Lower limb mass 2014 6 01 82 87 http://jams.arakmu.ac.ir/article-1-2621-en.pdf