Volume 20, Issue 8 (11-2017)
J Arak Uni Med Sci 2017, 20(8): 83-93 |
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Karimi F. Study of MDR1 Gene Expression in Colorectal Cancer Using Nanobiosensor. J Arak Uni Med Sci 2017; 20 (8) :83-93
URL: http://jams.arakmu.ac.ir/article-1-4921-en.html
URL: http://jams.arakmu.ac.ir/article-1-4921-en.html
Department of Biology, Faculty of Sciences, University of Maragheh, Maragheh, Iran , karimifm@maragheh.ac.ir
Abstract: (3301 Views)
Abstract
Background: Nowadays, drug resistances are the main problems in no response of cancer patients to drug. Identification of molecular mechanisms and causative agents of drug resistance can be important to determine the treatment method in different stages of disease. In this study, for the first time, expression of multidrug resistance (MDR1) gene was studied in colorectal cancer by Carbon Nanotubes (CNTs)-based nanobiosensor method.
Materials and Methods: At first, a nanobiosensor was designed based on carbon nanotubes (CNTs). After optimizing reaction condition to identify target DNA sequence, colorectal cancer patient’s cDNA samples were evaluated by nanbiosensor.
Results: After immobilizing the probe on CNTs, Fluorescence immersion was quenched but by adding complementary DNA, fluorescence again was observed. In hybridization reaction of cDNA with nanobiosensor high significant levels of fluorescence emission was observed in colorectal cancer tumor samples compared normal tissue indicating high level expression of MDR1gene in tumor tissue.
Conclusion: Finally, the evaluation of the expression of MDR1 gene by nanobiosensor indicated that in colorectal cancer tissue MDR1, mRNA level was higher than normal tissue. In addition, the results of this study indicated that carbon nanotubes-based nanobiosensor beside PCR-based method can be used as a very powerful tool for expression studies in human cancers at mRNA levels.
Background: Nowadays, drug resistances are the main problems in no response of cancer patients to drug. Identification of molecular mechanisms and causative agents of drug resistance can be important to determine the treatment method in different stages of disease. In this study, for the first time, expression of multidrug resistance (MDR1) gene was studied in colorectal cancer by Carbon Nanotubes (CNTs)-based nanobiosensor method.
Materials and Methods: At first, a nanobiosensor was designed based on carbon nanotubes (CNTs). After optimizing reaction condition to identify target DNA sequence, colorectal cancer patient’s cDNA samples were evaluated by nanbiosensor.
Results: After immobilizing the probe on CNTs, Fluorescence immersion was quenched but by adding complementary DNA, fluorescence again was observed. In hybridization reaction of cDNA with nanobiosensor high significant levels of fluorescence emission was observed in colorectal cancer tumor samples compared normal tissue indicating high level expression of MDR1gene in tumor tissue.
Conclusion: Finally, the evaluation of the expression of MDR1 gene by nanobiosensor indicated that in colorectal cancer tissue MDR1, mRNA level was higher than normal tissue. In addition, the results of this study indicated that carbon nanotubes-based nanobiosensor beside PCR-based method can be used as a very powerful tool for expression studies in human cancers at mRNA levels.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/02/1 | Accepted: 2017/09/25
Received: 2017/02/1 | Accepted: 2017/09/25
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