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Volume 17, Issue 12 (3-2015)                   J Arak Uni Med Sci 2015, 17(12): 1-8 | Back to browse issues page

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Pazoki H, Imani H, Shahverdi A, Farokhi F, Tahaei L S. The Effective of Cord Blood Platelet Extraction (PL) on Meiosis Resumption and Maturation of Mouse Oocyte at Germinal Vesicle stage (GV). J Arak Uni Med Sci. 2015; 17 (12) :1-8
URL: http://jams.arakmu.ac.ir/article-1-3206-en.html
1- Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , hasan_fsh33@yahoo.com
2- Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3- Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Abstract:   (5339 Views)

Background: Maturation of oocytes and fertilization in vitro can be considered as one of the most important steps to treat infertility. In this study maturation medium was enriched with Platelet extraction (PL) which has high concentration of growth factors. Meiosis resumption and maturation was monitored after 18 hours of maturation.

Materials and Methods: In this experimental study, oocytes at germinal vesicle stage were obtained from mature NMRI female mice. Maturation medium was &alphaMEM and the control groups had 5-10% FBS and the medium in the experimental groups was enriched by 5, 10% PL and the combination of 5% PL and 5% FBS. Meiosis resumption and maturation were observed after 18 hours.

Results: The rate of matured oocytes in the experimental group 5% PL for both COC and DO group was significantly higher than the control groups (P<0.05). The maturation rate for 5% PL was 61% for the COC group and 72% for the DO group while this rate for 5% FBS control group was 53% and 50% respectively.

Conclusion: PL had a significant effect on meiosis resumption and maturation of oocyte at germinal vesicle stage. Based on these results, PL could be used as a maturation promoting factor.

Full-Text [PDF 590 kb]   (1140 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2014/10/5 | Accepted: 2014/11/11

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