Write your message
Volume 18, Issue 3 (6-2015)                   J Arak Uni Med Sci 2015, 18(3): 28-36 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dorostkar R, Hashemzadeh M S, Tat M, Shafaati M R, Najarasl M, Zahiri Yeganeh S. Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer. J Arak Uni Med Sci. 2015; 18 (3) :28-36
URL: http://jams.arakmu.ac.ir/article-1-3712-en.html
1- Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran , R.dorost@yahoo.com
2- Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (8986 Views)

Background: In 1970, human papillomavirus (HPV) was introduced as the main etiologic factor of cervical carcinoma. Since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as PCR have particular importance in accurate, early and definite diagnosis of the virus. So, in this research, our goal is to use a proprietary PCR assay based on L1 gene of human papillomavirus for molecular recognition of HPV and to evaluate its prevalence in patient samples.

Materials and Methods: In this experimental study, after collecting of samples from malignant cervical lesions, the viral DNA was extracted from paraffin blocks of 50 clinical samples and PCR was done by specific primers for L1 gene of human papillomavirus in all samples. After the analysis of PCR products by 2% agarose gel electrophoresis, sensitivity and specificity of the test were also evaluated.

Results: Among 50 patient samples, 33 cases were confirmed to be positive for HPV infection and 17 cases were negative, showing high frequency of HPV in this patient population (about 66%). The results of specificity assay were positive for papilloma samples and sensitivity of the assay was 20 copies of recombinant construct containing L1 per reaction.

Conclusion: This study showed that PCR by specific primers for L1 gene of human papilloma virus is a proper and accurate method for detection of this virus and the results confirm the previous reports of correlation between HPV and cervical carcinoma.

Full-Text [PDF 998 kb]   (3008 Downloads)    
Type of Study: Original Atricle | Subject: General
Received: 2015/05/25 | Accepted: 2015/05/26

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2021 All Rights Reserved | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb