Write your message
Volume 19, Issue 10 (1-2017)                   J Arak Uni Med Sci 2017, 19(10): 25-38 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Savary F, Moazedi A A, Gharib-Naseri M K, Zadkarami M R. The Role of GABAA Receptor in Antispasmodic Activity of Hydroalcholic Extract of Petroselinum Crispum (Parsley) Seed in Rat Ileum. J Arak Uni Med Sci. 2017; 19 (10) :25-38
URL: http://jams.arakmu.ac.ir/article-1-4559-en.html
1- Department of Biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran , feryal.savary@yahoo.com
2- Department of Biology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3- Department of Physiology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4- Department of Statistics, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (3214 Views)

Abstract

Background: Parsley is one of the medicinal herbs used for gastrointestinal disorders. However, spasmolytic activity of Petroselinum crispum (parsley) extract has been reported, there is a lack of information to support the mechanism of this antispasmodic activity. Taking this into account, the purpose of the present work was to investigate the role of GABAA receptor on antispasmodic activity of the hydroalcoholic extract of parsley seed in isolated rat ileum.

Materials and Methods: In this study, terminal portion of ileum (2 cm) was dissected out and mounted in an organ bath containing air bubbled Tyrode solution (37οC, pH=7.4). Under 1gr resting tension, ileal contraction was induced by KCl (60 mM) and recorded isotonically. The effects of non-cumulative (0.1-0.5 mg/ml) concentrations of extract on KCl-induced contractions were examined.  After evaluating the effect of agonist and antagonist GABAA receptor, the effect of parsley extract was assessed in the presence of muscimol (25 µM) and bicuculline (10 µM) as agonist and antagonist of GABAA, respectively. 

Results: Parsley seed extract reduced the KCl-induced ileal contraction in a concentration-dependent manner (n=7, p<0.001). Both muscimol and bicuculline exerted relaxant effect on ileal contraction (n=7, p<0.05, p<0.01, respectively). Surprisingly, agonist and antagonist of GABAA both potentiated the spasmolytic effect of extract (0.2 mg/ml). Altogether, spasmolytic effect of extract was not attenuated in the presence of GABAA antagonist.

Conclusion: It seems that GABAA receptor is not involved in the antispasmodic effect of parsley seeds extract in rat ileum.

Full-Text [PDF 1227 kb]   (1339 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2016/07/27 | Accepted: 2016/10/26

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2021 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb