Volume 22, Issue 3 (8-2019)                   J Arak Uni Med Sci 2019, 22(3): 59-68 | Back to browse issues page

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Rezaei R, Fathi M. The Study of the Effect of A Long Term Endurance Activity on Cardiac Structure and Expression of Mir-133 in Rats. J Arak Uni Med Sci 2019; 22 (3) :59-68
URL: http://jams.arakmu.ac.ir/article-1-6019-en.html
1- Department of Sport Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2- Department of Physical Education and Sport Sciences, Faculty of Humanity Sciences, Lorestan University, Khoramabad, Iran. , fathi.m@lu.ac.ir
Abstract:   (2072 Views)
Background and Aim: Endurance training causes cardiac remodeling, one of the factors that adjusting expression of more genes of heart is miR-133. The aim of this study was to evaluate the effect of endurance training on miR-133 expression in wistar rats’ heart.
Materials and Methods: In this experimental study, 14 rats were housed under controlled conditions for 4 weeks, after familiarization they were randomly assigned to control (7 rats) and experimental (7 rats) groups. The experimental group performed 14 weeks, 6 session per week an endurance training program (that gradually reached to 60 min and 30 m/min) on treadmill. 48 hours after the end of the last session, all animals were anesthetized and sacrificed. Then, the their heart were removed and after tissue homogenization of left ventricle, and RNA extraction, and cDNA synthesis, the expression level of left ventricle miR-133 were measured by using Real-Time PCR. The rate of miR-133 expression was evaluated by using t-test at p≤ 0.05 level.
Ethical Considerations: This study was approved in Research Ethics Committee of Lorestan University with the code 1396345.52
Findings: After 14 weeks endurance training, the expression of heart miR-133 in experimental group was significantly increased (p=0.007) than control group which coincided with increase of the rate of left ventricular mass to weight body (p=0.012).
Conclusion: Regarded to structural changes of heart, it seems the part of heart adaptation to endurance exercise caused by increase in miR-133 expression.
Full-Text [PDF 597 kb]   (833 Downloads)    
Type of Study: Original Atricle | Subject: General
Received: 2019/02/9 | Accepted: 2019/05/15

1. Frey N, Olson EN. Cardiac hypertrophy: the good, the bad, and the ugly. Annu Rev Physiol. 2003; 65:45-79.
2. Care A, Catalucci D, Felicetti F, Bonci D, Addario A, Gallo P, et al. MicroRNA-133 controls cardiac hypertrophy. Nat Med. 2007; 13(5):613-8.
3. Latronico MV, Elia L, Condorelli G, Catalucci D. Heart failure: targeting transcriptional and post-transcriptional control mechanisms of hypertrophy for treatment. Int J Biochem Cell Biol. 2008; 40(9):1643-8.
4. Hill JA, Olson EN. Cardiac plasticity. N Engl J Med. 2008;358(13):1370-80.
5. Silva D, Carneiro FD, Almeida KC, Fernandes-Santos C. Role of miRNAs on the Pathophysiology of Cardiovascular Diseases. Arquivos brasileiros de cardiologia. 2018; 111(5):738-46.
6. Zhou SS, Jin JP, Wang JQ, Zhang ZG, Freedman JH, Zheng Y, et al. miRNAS in cardiovascular diseases: potential biomarkers, therapeutic targets and challenges. Acta pharmacologica Sinica. 2018; 39(7):1073-84.
7. Oliveto S, Mancino M, Manfrini N, Biffo S. Role of microRNAs in translation regulation and cancer. World journal of biological chemistry. 2017; 8(1):45-56.
8. O'Brien J, Hayder H, Zayed Y, Peng C. Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation. Frontiers in endocrinology. 2018; 9:402.
9. Siracusa J, Koulmann N, Banzet S. Circulating myomiRs: a new class of biomarkers to monitor skeletal muscle in physiology and medicine. Journal of cachexia, sarcopenia and muscle. 2018; 9(1):20-7.
10. McCarthy JJ, Esser KA. MicroRNA-1 and microRNA-133a expression are decreased during skeletal muscle hypertrophy. Journal of Applied Physiology. 2007; 102(1):306-13.
11. Li N, Zhou H, Tang Q. miR-133: A Suppressor of Cardiac Remodeling? Frontiers in pharmacology. 2018; 9:903.
12. Zhao Y, Ransom JF, Li A, Vedantham V, von Drehle M, Muth AN, et al. Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2. Cell. 2007; 129(2):303-17.
13. Chen JF, Mandel EM, Thomson JM, Wu QL, Callis TE, Hammond SM, et al. The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nature Genetics. 2006; 38(2):228-33.
14. Xu C, Lu Y, Pan Z, Chu W, Luo X, Lin H, et al. The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes. Journal of cell science. 2007; 120(Pt 17):3045-52.
15. Ekhteraei Tousi S, Mohammad Soltani B, Sadeghizadeh M, Hoseini S, Soleimani M. Hsa-miR-133b Expression Profile during Cardiac Progenitor Cell Differentiation and its Inhibitory Effect on SRF Expression. Pathobiology Research. 2013; 16(1):1-9.
16. Thum T, Bauersachs J. MicroRNAs in cardiac hypertrophy and failure. Drug Discovery Today: Disease Mechanisms. 2009; 5(3-4):e279-e83.
17. Chen JF, Callis TE, Wang DZ. microRNAs and muscle disorders. Journal of Cell Science. 2008; 122(1):13-20.
18. Berry MJ, Sheilds KL, Adair NE. Comparison of Effects of Endurance and Strength Training Programs in Patients with COPD. Copd. 2018; 15(2):192-9.
19. Nystoriak MA, Bhatnagar A. Cardiovascular Effects and Benefits of Exercise. Frontiers in cardiovascular medicine. 2018; 5:135.
20. Xu X, Wan W, Powers AS, Li J, Ji LL, Lao S, et al. Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats. J Mol Cell Cardiol. 2008; 44(1):114-22.
21. FARRIOL M, ROSSELL J, SCHWAR S. Body surface area in Sprague-Dawley rats. Journal of Animal Physiology and Animal Nutrition. 1997; 77 (0931-2439):61-5.
22. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45.
23. Wang L, Lv Y, Li G, Xiao J. MicroRNAs in heart and circulation during physical exercise. Journal of sport and health science. 2018; 7(4):433-41.
24. Zhang S, Chen N. Regulatory Role of MicroRNAs in Muscle Atrophy during Exercise Intervention. International journal of molecular sciences. 2018; 19(2).
25. Soci UP, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, et al. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiol Genomics. 2011; 43(11):665-73.
26. Davidsen PK, Gallagher IJ, Hartman JW, Tarnopolsky MA, Dela F, Helge JW, et al. High responders to resistance exercise training demonstrate differential regulation of skeletal muscle microRNA expression. Journal of Applied Physiology. 2011; 110(2):309-17.
27. Czubryt MP, Olson EN. Balancing contractility and energy production: the role of myocyte enhancer factor 2 (MEF2) in cardiac hypertrophy. Recent Prog Horm Res. 2004; 59:105-24.
28. Nelson TJ, Balza R, Jr., Xiao Q, Misra RP. SRF-dependent gene expression in isolated cardiomyocytes: regulation of genes involved in cardiac hypertrophy. J Mol Cell Cardiol. 2005; 39(3):479-89.
29. Trachsel LD, Ryffel CP, De Marchi S, Seiler C, Brugger N, Eser P, et al. Exercise-induced cardiac remodeling in non-elite endurance athletes: Comparison of 2-tiered and 4-tiered classification of left ventricular hypertrophy. PLoS One. 2018; 13(2):e0193203.
30. Fulghum K, Hill BG. Metabolic Mechanisms of Exercise-Induced Cardiac Remodeling. Frontiers in cardiovascular medicine. 2018; 5:127.

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