Volume 28, Issue 6 (1-2026)
J Arak Uni Med Sci 2026, 28(6): 0-0 |
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Soltani M, Shojafar E, Ghafarizadeh A A, Moslemi A, Baazm M. The Effect of Platelet-Rich Plasma on Parameters of Frozen‐Thawed Sperm in Asthentertospermia. J Arak Uni Med Sci 2026; 28 (6)
URL: http://jams.arakmu.ac.ir/article-1-7932-en.html
URL: http://jams.arakmu.ac.ir/article-1-7932-en.html
1- MSc, Students Research Committee, Arak University of Medical Sciences, Arak, Iran
2- PhD, Rastak Infertility Treatment Center, Sina Hospital, Arak, Iran
3- PhD, Department of Biostatistics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
4- PhD, Department of Anatomy, School of Medicine, AND, PhD, Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran ,maryam.baazm@gmail.com
2- PhD, Rastak Infertility Treatment Center, Sina Hospital, Arak, Iran
3- PhD, Department of Biostatistics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
4- PhD, Department of Anatomy, School of Medicine, AND, PhD, Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran ,
Abstract: (53 Views)
Introduction: Sperm cryopreservation is a crucial method for preserving fertility in patients with asthenoteratozoospermia. However, this process can lead to a reduction in sperm parameters due to the production of free radicals and damage to the cell membrane. Various substances are added to the cryopreservation medium to prevent cellular damage. In this study, platelet-rich plasma (PRP), which contains growth factors and bioactive molecules, was used to improve sperm parameters after freezing.
Methods: Semen samples were collected from 20 men diagnosed with asthenoteratozoospermia. The samples were randomly divided into five groups: control (no PRP), PRP50, PRP100, PRP200, and PRP400. Homologous PRP was prepared and added to the respective groups. The sperm samples were cryopreserved in liquid nitrogen. Twenty days after freezing, samples were thawed and subjected to a comprehensive evaluation of viability, motility, morphology, malondialdehyde (MDA) levels, and DNA fragmentation using specific assay kits. The findings were evaluated using one-way analysis of variance followed by Tukey's post hoc test.
Results: The results of this study demonstrated that cryopreservation led to a significant decrease in all sperm parameters in the control group. The addition of PRP at concentrations of 50 and 400 among the concentrations used resulted in a significant increase in total motility, progressive and non-progressive motility, sperm viability, and a decrease in immotile sperm, DNA fragmentation, and MDA levels compared to the control group (p<0.05).
Conclusions: Cryopreservation and subsequent thawing can have detrimental effects on the biological properties of sperm samples. Therefore, the dose-dependent addition of platelet-rich plasma as a cryoprotectant may offer a promising approach to mitigate the negative impacts of freezing on samples from men with asthenoteratozoospermia.
Methods: Semen samples were collected from 20 men diagnosed with asthenoteratozoospermia. The samples were randomly divided into five groups: control (no PRP), PRP50, PRP100, PRP200, and PRP400. Homologous PRP was prepared and added to the respective groups. The sperm samples were cryopreserved in liquid nitrogen. Twenty days after freezing, samples were thawed and subjected to a comprehensive evaluation of viability, motility, morphology, malondialdehyde (MDA) levels, and DNA fragmentation using specific assay kits. The findings were evaluated using one-way analysis of variance followed by Tukey's post hoc test.
Results: The results of this study demonstrated that cryopreservation led to a significant decrease in all sperm parameters in the control group. The addition of PRP at concentrations of 50 and 400 among the concentrations used resulted in a significant increase in total motility, progressive and non-progressive motility, sperm viability, and a decrease in immotile sperm, DNA fragmentation, and MDA levels compared to the control group (p<0.05).
Conclusions: Cryopreservation and subsequent thawing can have detrimental effects on the biological properties of sperm samples. Therefore, the dose-dependent addition of platelet-rich plasma as a cryoprotectant may offer a promising approach to mitigate the negative impacts of freezing on samples from men with asthenoteratozoospermia.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2025/01/13 | Accepted: 2025/04/9
Received: 2025/01/13 | Accepted: 2025/04/9
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