Volume 28, Issue 6 (1-2026)
J Arak Uni Med Sci 2026, 28(6): 0-0 |
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Khodavandi A, Alizadeh F, Rastgo P. Efficacy of Thymus vulgaris and Trigonella foenum-graecum Extracts Alone and in Combination in Candida albicans Hyphae Model and in Downregulation of Secreted Aspartyl Proteinase 1 Gene Expression. J Arak Uni Med Sci 2026; 28 (6)
URL: http://jams.arakmu.ac.ir/article-1-7963-en.html
URL: http://jams.arakmu.ac.ir/article-1-7963-en.html
1- Department of Microbiology, Gac. C., Islamic Azad University, Gachsaran, Iran
2- Department of Microbiology, Gac. C., Islamic Azad University, Gachsaran, Iran ,fahimeh.alizadeh@iau.ac.ir
3- Department of Microbiology, Yas. C., Islamic Azad University, Yasuj, Iran
2- Department of Microbiology, Gac. C., Islamic Azad University, Gachsaran, Iran ,
3- Department of Microbiology, Yas. C., Islamic Azad University, Yasuj, Iran
Abstract: (73 Views)
Introduction: The emergence of antifungal resistance in Candida albicans diseases poses a threat to global public health. New treatments are needed to target C. albicans and its ability to produce hyphae. The aim of this study was to investigate the potential synergistic effects and antifungal properties of Thymus vulgaris and Trigonella foenum-graecum extracts alone and in combination on C. albicans.
Methods: In this experimental study, extracts of Thymus vulgaris and Trigonella foenum-graecum were prepared using hot water (60 °C) and Soxhlet extraction with methanol (10%). Yeast susceptibility testing was performed according to the Clinical and Laboratory Standards Institute disk diffusion and broth microdilution guidelines. The hyphal model was created in the presence of alcoholic extracts of Thymus vulgaris and Trigonella foenum-graecum alone and in combination. Crystal violet staining, microscopic observation and gene expression analysis were used to evaluate the inhibition of hyphal growth.
Results: The results showed that 90% of the C. albicans isolates were resistant to fluconazole. Aqueous and alcoholic extracts of Thymus vulgaris in combination with Trigonella foenum-graecum showed synergistic, partially synergistic and additive effects. Alcoholic extracts of Thymus vulgaris with Trigonella foenum-graecum alone and in combination have anti-hyphae activity by reducing the percentage of hyphae, reducing the number of planktonic cells and the transition of planktonic cells to hyphae, and down-regulating the Secreted Aspartyl Proteinase 1 (SAP1) gene.
Conclusions: Taken together, these results indicate that extracts of Thymus vulgaris alone and in combination with Trigonella foenum-graecum may offer a potent alternative strategy to combat resistant C. albicans infections and their ability to reduce hyphae formation. Additionally, the SAP1 gene could be a likely target in the synergistic interaction of alcoholic extracts of Thymus vulgaris in combination with Trigonella foenum-graecum against the C. albicans hyphae model.
Methods: In this experimental study, extracts of Thymus vulgaris and Trigonella foenum-graecum were prepared using hot water (60 °C) and Soxhlet extraction with methanol (10%). Yeast susceptibility testing was performed according to the Clinical and Laboratory Standards Institute disk diffusion and broth microdilution guidelines. The hyphal model was created in the presence of alcoholic extracts of Thymus vulgaris and Trigonella foenum-graecum alone and in combination. Crystal violet staining, microscopic observation and gene expression analysis were used to evaluate the inhibition of hyphal growth.
Results: The results showed that 90% of the C. albicans isolates were resistant to fluconazole. Aqueous and alcoholic extracts of Thymus vulgaris in combination with Trigonella foenum-graecum showed synergistic, partially synergistic and additive effects. Alcoholic extracts of Thymus vulgaris with Trigonella foenum-graecum alone and in combination have anti-hyphae activity by reducing the percentage of hyphae, reducing the number of planktonic cells and the transition of planktonic cells to hyphae, and down-regulating the Secreted Aspartyl Proteinase 1 (SAP1) gene.
Conclusions: Taken together, these results indicate that extracts of Thymus vulgaris alone and in combination with Trigonella foenum-graecum may offer a potent alternative strategy to combat resistant C. albicans infections and their ability to reduce hyphae formation. Additionally, the SAP1 gene could be a likely target in the synergistic interaction of alcoholic extracts of Thymus vulgaris in combination with Trigonella foenum-graecum against the C. albicans hyphae model.
Keywords: Candida albicans, Thymus vulgaris and Trigonella foenum-graecum in combination, SAP1 gene expression
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2025/02/16 | Accepted: 2025/05/18
Received: 2025/02/16 | Accepted: 2025/05/18
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