Volume 14, Issue 7 (Brucellosis Supplement 2012)
J Arak Uni Med Sci 2012, 14(7): 71-77 |
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Farazi A A, Hosseini S D. Diagnostic validity of the conventional brucellosis serological tests in. J Arak Uni Med Sci 2012; 14 (7) :71-77
URL: http://jams.arakmu.ac.ir/article-1-1387-en.html
URL: http://jams.arakmu.ac.ir/article-1-1387-en.html
1- , hosseinida@yahoo.com
Abstract: (11701 Views)
Background: Brucellosis is a zoonotic disease in humans and animals that its control is dependent on the prevalence of diseases in animal populations. The purpose of this study was to compare the sensitivity and specificity of common diagnostic tests for brucellosis.
Materials and Methods: In an analytical cross-sectional study, 297 serum samples from an infected herd that had a history of abortion were prepared and evaluated through standard tube agglutination, Rose Bengal, 2-Mercaptoethanol, complement fixation, and ELISA tests. In addition, microbial culture was done for positive serum samples, lymph nodes, and infected organs.
Results: Based on the total 297 serum samples tested, the sensitivities for standard tube agglutination, Rose Bengal, 2-Mercaptoethanol, complement fixation, and ELISA tests were 89, 81.5, 75.3, 89.7, and 93.2% , whereas specificities were determined to be 97.4, 94, 96, 98, and 99.3%, respectively.
Conclusion: The findings of this study show that the standard tube agglutination test, complement fixation test, and ELISA are more sensitive than other tests. Moreover, ELISA test was the most sensitive and specific serological test for diagnosis of brucellosis in the present study
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