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Volume 16, Issue 1 (4-2013)                   J Arak Uni Med Sci 2013, 16(1): 34-42 | Back to browse issues page

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Hasanzadeh L, Abtahi H, Ghaznavi-Rad E, Soufian S, Farjadi V. Productnin of Antigenic Region VacA Helicobacter Pylori in E.coli . J Arak Uni Med Sci. 2013; 16 (1) :34-42
URL: http://jams.arakmu.ac.ir/article-1-1923-en.html
1- Department of Biotechnology and Microbiology, Arak University of Medical Sciences, Arak, Iran
2- Department of Microbiology, Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran , abtahi@arakmu.ac.ir
3- Department of Microbiology, Arak University of Medical Sciences, Arak, Iran
4- Department of Biology, Payame Noor University, Arak, Iran
5- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
Abstract:   (10097 Views)

Background: Helicobacter pylori is the most common bacteria causing chronic infections worldwide. An important virulence factor of H. pylori is a vacuolating cytotoxin (VacA) that induces the formation of acidic vacuoles in cytoplasm and damage to epithelial cells. The aim of this study was to examine the antigenic properties of the recombinant VacA of H. pylori in infected sera of mice and human.

Materials and Methods: In this experimental study, the highly antigenic region of VacA gene (1233 bp) was detected by bioinformatics methods, and it was amplified by PCR method and cloned into the pET32a expression vector. After expression and purification of the target protein, its antigenicity was studied by Western Blotting using human sera infected with H. pylori and sera from immunized mice infected with purified recombinant VacA.

Results: PCR and sequencing results showed that the target gene was correctly cloned into the recombinant vector. Antibodies used in Western Blotting indicated the production and expression of the recombinant protein (65kDa) with concentration of 2.1 mg/ml.

Conclusion: Recombinant VacA protein has antigenic and immunogenic properties thus, it is a proper candidate for designing H. pylori vaccine and diagnostic kits

Full-Text [PDF 321 kb]   (1650 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2012/08/28 | Accepted: 2013/08/28

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