Volume 11, Issue 2 (6-2008)                   J Arak Uni Med Sci 2008, 11(2): 102-110 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hatefi M, Mehrabian S, Nouhi A S, Rafiee Tabatabaee R. Survey of antimutagenic effects of ethanolic extract of propolis by Salmonella typhymurium/microsome. J Arak Uni Med Sci 2008; 11 (2) :102-110
URL: http://jams.arakmu.ac.ir/article-1-231-en.html
1- , mit_hatefi@yahoo.com
2- Tarbiat Moalem University
Abstract:   (21584 Views)
Introduction: In this study, antimutagenesis effect of ethanolic extract of propolis by Ames test against two mutagenic substances named azide sodium and potassium permanganate in the presence and the absence of microsomal homogenate of mouse liver (S9) has been investigated. Materials and Methods: In this experimental study at first, different concentrations of ethanolic extract of propolis (0.1-5%) for determining minimum inhibitory concentration (MIC) against tester strains were used. Then by Ames test, antimutagenesis effect was assessed in nontoxic extent. In this test, various strains of Salmonella typhymurium (TA100 and TA97) that contained selective mutation in their operon histidine, were used. Mutant strains (His-) were grown on culture media containing minimum salt and glucose in the presence of mutagen substances above. So only those bacteria that were reversed by mutation (His+) could grow and form colonies on culture media. If antimutagen (EEP) and mutagen substances were gathered, reversed mutation would be reduced and the rate of mutation inhibition could be calculated by means of formula. The differences between the averages of revertants per plate of the sample in relation to the mutagens were analyzed using SPSS software and one-way ANOVA. Results: The resulted MIC values clearly showed that ethanolic extract of propolis at 5% concentration has antibacterial activity against Salmonella typhymurium, but in 0.1-4% concentrations, such effects were not seen. Findings also showed that propolis in such concentrations could neutralize mutagenic effects of those substances in a dose dependent manner. Conclusion: Finally we found that ethanolic extract of propolis that contains different kinds of major and important substances like flavonoids, has good antimutagenic effects and the best concentration for obtaining such effect is in 4% which also was confirmed with microsomal results. The mechanism of antibacterial effect of propolis is complex and it has no analogy to any classic antibiotics, but it should be emphasized that bacterial cell division is inhibited by propolis. Some researchers also argue that propolis could inhibit DNA-dependent RNA polymerase.
Full-Text [PDF 220 kb]   (3099 Downloads)    
Subject: General
Received: 2009/03/10

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb