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Volume 17, Issue 7 (10-2014)                   J Arak Uni Med Sci 2014, 17(7): 59-70 | Back to browse issues page

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Dorostkar H R, Ansari Hadipour H, Goodarzi M T. Relationship Between Structural Changes of Hemoglobin with Oxidative Status of Plasma in Diabetic Hemodialysis Patients. J Arak Uni Med Sci. 2014; 17 (7) :59-70
URL: http://jams.arakmu.ac.ir/article-1-2709-en.html
1- Student Research Committee, Arak University of Medical Sciences Arak, Iran
2- Department of Biochemistry and Genetics, Arak University of Medical Sciences, Arak, Iran , ansary@arakmu.ac.ir
3- Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran
Abstract:   (4951 Views)

Background: There is a relationship between diabetes, dialysis and oxidative stress. The aim of this study was the comparison of structural changes in Hb, oxidative damages in plasma proteins, and antioxidant capacity in diabetic hemodialysis patients with those of control subjects.

Materials and Methods: In this experimental study, blood samples obtained from diabetic hemodialysis patients and control group. Oxidative damages in plasma proteins were determined by carbonyl assay and antioxidant power of plasma was performed by FRAP assay. Conformational changes in Hb were detected by spectrophotometric analysis. Blood glucose, urea, creatinine and uric acid in patients were determined usnig routine laboratory methods. Statistical analysis were performed by using regression analysis and t-test in SPSS20 software.

Results: This study showed a significant correlation between carbonyl content of plasma proteins and optical density of Hb at 630 and 275 nm which corresponds to structural changes in Hb. ferric reducing ability of plasma (FRAP), as an index of total antioxidant capacity of plasma was found to be enhanced significantly in diabetic patients receiving hemodialysis (from 1019.62±129 to 1354.54±129 molare, p<0.05).

Conclusion: The results obtained in the present study showed that inducible factors in diabetic hemodialysis patients contribute to plasma antioxidant activity and probably responsible for prevention of carbonyl formation and oxidative damages in hemoglobin.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2013/12/21 | Accepted: 2014/04/9

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