Background : Urine tract infection(UTI) among children is the most prevalent bacterial infection and the second prevalent infection after Viral Flu.Regarding this cfact that urine culturing has very important role in diagnisis and perseverance of this disease and sampling method has significant effect on this disease remedy.

  In this study the effect of genital area ablution on decreasing the contamination of urine culture is examined.

  Methods and Materials: This study is in the form of case_controlled.In this study 620 little girls (3-12 years –old) referring to Amir Kabir children special clinic are examined.Population under investigation was cpatients who had not history of taking antibiotic during last seven days, sondage and genital anomaly and inflammation and genital discharge. 

  Patients were randomly entered in two different groups one with ablution and the other without ablution.After data collection, available inputs were statistically analyzed with SPSS and the effect of genital area ablution was determined on decreasing the contamination of urine culture.

  Results: Among 310 patients in with_ablution group 11 patients were reported UTI(3/7%).3 patients were contaminated (1%),296 patients were normal (95/3%).Among 310 patients in the second group,14 patients were reported UTI(4/7%),6 patients were contaminated(2%),290 patients were normal(93/3%).Contamination rate in with_ablution group were 1% and in the other group was 2%Based on Pvalue :0/491 in both groups no significant statistically difference were found between these two groups and the analysis result distribution in both groups in a=0/05 level were statistically the same.

  Conclusion: In both group no significant statistically difference were found between these two groups ,so we concluded that the genital area ablution doesn’t have such an effect on decreasing the contamination of urine culture and we don’t suggest ablution of genital area in children before providing urine sampling.