Background: The current study aims to investigate the impact of high intensity interval training (HIIT) on serum levels of CK and LDH as the muscle damage indicators and on Gelatinase-A (MMP-2) serum levels as the tissae inflammatory marker among young sedentary girls.

Materials and Methods: For this quasi-experimental study, 14 sedentary female college students were selected and randomly divided into two groups including the exercise HIIT group (means and standard deviations of age: 21.28 ± 2.56 (years)  weight:52.86 ±4.95 (kg) and height: 163.1±3.7 (cm)) and the control group (means and standard deviations of age: 20.25 ±7.50 (years) weight:52.64 ±3.67 (kg) and height: 162.4±4.5 (cm)). The experimental group performed six repetitions of one-minute runs at 90%- 95% of HRmax. The blood samples were collected before and 30 minutes after the exercise protocol. The serum CK, LDH and MMP-2 levels were measured using corresponding kits. The data were analyzed through the independent t-test at the significance level of 0.05 (p<0.05).

Results: After collecting and analyzing Data, the results showed that CK and LDH levels increased significantly after performing HIIT, while there was no significant change in MMP-2 due to the HIIT.

Conclusion: It can be concluded that the HIIT protocol will lead to an increase in some indicators of muscle damage such as CK, LDH, and that no significant changes could be observed for MMP-2 as the body's inflammation response.