Volume 19, Issue 1 (4-2016)                   J Arak Uni Med Sci 2016, 19(1): 32-43 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shokouhi H, Zolfaghari M R, Farahmand B, Tabatabaeian M, Taheri N, Fotouhi F. Immunological Assessment of Three Tandem Repeat of Influenza Virus M2 Extracellular Domain with Adjuvant in Balb/c Mice Model. J Arak Uni Med Sci 2016; 19 (1) :32-43
URL: http://jams.arakmu.ac.ir/article-1-3803-en.html
1- Department of Microbiology, Qom Branch, Islamic Azad University,Qom, Iran.
2- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
3- Influenza Research Laboratory, Pasteur Institute of Iran, Tehran, Iran.
4- Influenza Research Laboratory, Pasteur Institute of Iran, Tehran, Iran. , fotouhi@pasteur.ac.ir
Abstract:   (5414 Views)

Background: Influenza A viruses are globally important respiratory pathogens which cause a high degree of morbidity and mortality during annual epidemics. M2 protein which expressed on the viral surface facilitates virus entry to the host cells. The extracellular domain of M2 protein (M2e) consists of N-terminal 24 residue which shows remarkable conservation among all subtypes of influenza A viruses. In this study, we evaluated the immunogenicity of three tandem repeats of M2e along with different adjuvants in BALB/C mice model.

Materials and Methods: Recombinant protein (3M2e) was expressed in Escherichia coli and purified. Six weeks old BALB/c mice were immunized interdermally with three doses of 3M2e alone or supplemented with Alum/CpG motif as adjuvant. Control group was injected with PBS. Two weeks after the last immunization, specific anti-M2 was measured using ELISA method and finally mice were challenged with one lethal dose (LD90) of PR8 virus.

Results: The results showed that 3M2e can induce specific antibody alone. However, 3M2e protein supplemented with Alum-CpG induced higher level of specific antibodies, so that, there was a significant difference with 3M2e group (p<0.05). Anti-M2 antibodies mostly consisted of IgG2a subclass which considered as activity index of TH1 Cells. Moreover, this group showed enhanced protection against wild-type virus (survival rate=60%).

Conclusion: Applying Alum-CpG as a complex adjuvant may play a crucial role in integrating innate and acquisitive immunity. We increased density of M2e in combination with complex adjuvant and showed that this vaccine induced power immune responses and semi-protected mice against lethal challenge.

Full-Text [PDF 762 kb]   (1424 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2015/06/27 | Accepted: 2015/11/16

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb