Volume 19, Issue 4 (7-2016)                   J Arak Uni Med Sci 2016, 19(4): 42-50 | Back to browse issues page

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Alimohammadi M, Yeganeh F, Haji Molla Hoseini M. Downregulating the Expression of CHID1 by Chitin Microparticles Mixed Leukocyte Culture. J Arak Uni Med Sci 2016; 19 (4) :42-50
URL: http://jams.arakmu.ac.ir/article-1-4219-en.html
1- Department of Immunology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Department of Immunology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , hajimolahoseini@yahoo.com
Abstract:   (3810 Views)

Background: chitin and its derivates microparticles (MPs) have immunomodulatory activities. In this study, we examined the effect of size, purity and acetylation degree of chitin MPs on CHID1- encoding SI-CLP, involved in inflammation- gene expression in mixed leukocyte culture.

Materials and Methods: Small (<40m) and medium(40-70m) sized chitin MPs were prepared by sonication, and they were used in treatment of leukocyte mixed culture in comparison with chitosan and also shrimp shell small-sized MPs. Neutral red uptake assay and microscopic examination of apoptosis were used to assess cytotoxicity of MPs. Finally, following cell treatment with MPs (100 μg/mL) for 48h, expression levels of CHID1 gene were determined by Real Time PCR.

Results: Different concentrations of chitinous MPs hadn’t any cytotoxic effects. In gene expression analysis, small-sized chitin MPs (<40 µ) resulted in down regulation of CHID1 gene expression (p=0.004), while other MPs didn’t change it significantly.

Conclusion: Size, purity and acetylation degree of chitin MPs influence their interference in immune cells interactions and it seems small-sized chitin MPs can potentially modulate immune responses through decreasing CHID1 gene expression. Using small-sized chitin MPs may be effective to treat allergies which their treatment strategies rely on modulating the immune responses.

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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2016/01/27 | Accepted: 2016/04/30

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