Background: Few studies have examined the effects of calcium plus vitamin D supplementation on metabolic profiles in overweight women with polycystic ovary syndrome (PCOS).This study was conducted to determine the effects of calcium plus vitamin D supplementation on metabolic profiles among overweight women with PCOS.

Materials and Methods: This randomized double-blind placebo-controlled clinical trial was conducted among 104 overweight and obess women with PCOS. Participants were randomly assigned into four groups to receive: 1) 1000 mg/d calcium+ vitamin D placebo; 2) 50000 IU/weeks vitamin D; 3) 1000 mg calcium/d and 4) calcium placebo + vitamin D placebo for 8 weeks. Fasting blood samples were taken at baseline and after 8 weeks intervention to measure metabolic profiles.

Results: After administrating calcium plus vitamin D supplementation, a significant decrease was observed in serum insulin (-3.3±11.4 vs.-1.1±8.5, -1.4±3.0 and +3.1±6.1 µIU/ml, p=0.03), insulin resistance (-0.7±2.4 vs.-0.3±2.5, -0.2±0.6 and +0.8±1.9, p=0.04), malondialdehyde (-0.6±0.3 vs.-0.1±0.2, -0.5±0.2 and +0.6±0.4 µmol/L, p=0.009) and a significant rise was seen in total antioxidant capacity (+35.2±21.7 vs.+22.5±37.7, +21.1±34.1 and -153.8±65.2 mmol/L, p=0.006) and glutathione (+216.0±79.3 vs.-47.5±60.2, +3.9±31.3 and -160.8±82.9 µmol/L, p=0.001) compared with vitamin D, calcium and placebo groups.

Conclusion: In conclusion, calcium plus vitamin D supplementation for eight weeks among women with PCOS had beneficial effects on metabolic profiles.