Volume 20, Issue 5 (8-2017)
J Arak Uni Med Sci 2017, 20(5): 71-78 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khajehnasiri N, Khazali H, Farzam Sheikhzadeh Hesari F. Alteration of neurokinin B gene expression and hypothalamic-pituitary- gonadal axis in response to one-month regular moderate physical activity. J Arak Uni Med Sci 2017; 20 (5) :71-78
URL: http://jams.arakmu.ac.ir/article-1-5003-en.html
URL: http://jams.arakmu.ac.ir/article-1-5003-en.html
1- Department of Animal Sciences, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran
2- Department of Animal Sciences, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran ,Homkhazali@yahoo.com
3- Department of Animal Sciences, Faculty of Natural Sciences, Tabriz University, Tabriz, Iran
2- Department of Animal Sciences, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran ,
3- Department of Animal Sciences, Faculty of Natural Sciences, Tabriz University, Tabriz, Iran
Abstract: (4088 Views)
Abstract
Background: The advantageous effects of the regular moderate physical activity (Exercise) on reproduction is widely accepted, but until now, the alterations of the expression of hypothalamic arcuate nuclei gene affecting on reproduction axis in response to this type of physical activity are not clear. Therefore, the goal of the present investigation was to study the effect of one –month regular moderate physical activity on neurokinin B gene expression and reproductive axis hormonal changes.
Materials and Methods: In the experimental study, fourteen adult male Wistar rats were randomly divided into control and one- month regular moderate exercise groups. After one - month physical activity (20m/min), the arcuate nucleus was isolated from brain and stored in -80 refrigerators for neurokinin B gene expression assay by Real-time PCR method. In addition, serum samples were taken to assess the corticosterone, luteinizing hormone and testosterone levels by ELISA method. Data were analyzed by Independent t-test in SPSS.
Results: Neurokinin-B gene expression level was lower in the group which received physical activity than the control group(p<0.05). Also, corticosterone serum concentration was decreased in the physical activity group(p<0.05). By contrast, the physical activity induced luteinizing hormone and Testosterone serum levels evaluation in exersice group compared with control group (p<0.05).
Conclusion: Regular moderate physical activity may improve male reproductive performance by reducing the corticosterone hormone level and decreasing neurokinin B expression.
Background: The advantageous effects of the regular moderate physical activity (Exercise) on reproduction is widely accepted, but until now, the alterations of the expression of hypothalamic arcuate nuclei gene affecting on reproduction axis in response to this type of physical activity are not clear. Therefore, the goal of the present investigation was to study the effect of one –month regular moderate physical activity on neurokinin B gene expression and reproductive axis hormonal changes.
Materials and Methods: In the experimental study, fourteen adult male Wistar rats were randomly divided into control and one- month regular moderate exercise groups. After one - month physical activity (20m/min), the arcuate nucleus was isolated from brain and stored in -80 refrigerators for neurokinin B gene expression assay by Real-time PCR method. In addition, serum samples were taken to assess the corticosterone, luteinizing hormone and testosterone levels by ELISA method. Data were analyzed by Independent t-test in SPSS.
Results: Neurokinin-B gene expression level was lower in the group which received physical activity than the control group(p<0.05). Also, corticosterone serum concentration was decreased in the physical activity group(p<0.05). By contrast, the physical activity induced luteinizing hormone and Testosterone serum levels evaluation in exersice group compared with control group (p<0.05).
Conclusion: Regular moderate physical activity may improve male reproductive performance by reducing the corticosterone hormone level and decreasing neurokinin B expression.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/03/31 | Accepted: 2017/07/22
Received: 2017/03/31 | Accepted: 2017/07/22
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
