Volume 20, Issue 7 (10-2017)
J Arak Uni Med Sci 2017, 20(7): 43-53 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sadeghi Z, Ebrahimi A, Davari Tanha F, Seyedena Y. Study of 4G/5G Mutations Prevalence in Plasminogen Activator Inhibitor-1 (PAI-1) Gene in Iranian Women with Ectopic Pregnancy. J Arak Uni Med Sci 2017; 20 (7) :43-53
URL: http://jams.arakmu.ac.ir/article-1-5175-en.html
URL: http://jams.arakmu.ac.ir/article-1-5175-en.html
1- Department of Cellular and Molecular, Faculty of Biology Sciences, Tehran-North Branch, Islamic Azad University, Tehran, Iran
2- PhD of Molecular Genetics, Post-doctoral of Genetic Complex Diseases, Cellular and Molecular Research Center, Institute of Endocrinology and Metabolism, Shahid Beheshti University of Medical Sciences, Tehran, Iran , ae35m@yahoo.com
3- MD, Specialist of Obstetrics and Gynecology, Fellowship of Infertility, Department of Obstetrics & Gynecology, Vali-e-Asr Reproductive Health Center, Faculty of Medical Sciences, University of Medical Sciences, Tehran, Iran
4- PhD of Human Genetics, Department of Cellular and Molecular, Faculty of Biology Sciences, Tehran-North Branch, Islamic Azad University, Tehran, Iran
2- PhD of Molecular Genetics, Post-doctoral of Genetic Complex Diseases, Cellular and Molecular Research Center, Institute of Endocrinology and Metabolism, Shahid Beheshti University of Medical Sciences, Tehran, Iran , ae35m@yahoo.com
3- MD, Specialist of Obstetrics and Gynecology, Fellowship of Infertility, Department of Obstetrics & Gynecology, Vali-e-Asr Reproductive Health Center, Faculty of Medical Sciences, University of Medical Sciences, Tehran, Iran
4- PhD of Human Genetics, Department of Cellular and Molecular, Faculty of Biology Sciences, Tehran-North Branch, Islamic Azad University, Tehran, Iran
Abstract: (4535 Views)
Abstract
Background: Ectopic pregnancy is a type of pregnancy in which implantation of zygote occurs out of the uterine cavity. One of the most important problems is bleeding. On the other hand, Plasminogen Activator Inhibitor-1(PAI-1) gene is one of the involved factors in unsuccessful pregnancies, and 4G/5G polymorphism is the most common changes of this gene. So, it is important to study the prevalence of these changes in this gene in women with ectopic pregnancy.
Materials and Methods: In this case-control study, 100 Iranian women with ectopic pregnancy and 101 Iranian women with the normal pregnancy were selected. After blood sampling, ARMS PCR method has been used for detection 4G/5G polymorphism and data were analyzed by statistical analysis.
Results: In this study, 4G allele with 70.79% prevalence and 5G allele with 63.5% prevalence are the most common alleles for the control and case group, respectively. 4G/4G and 4G/5G genotypes in the control group and 4G/5G and 5G/5G genotypes in the case group are prevalent. An Armitage test found p<0.05 for both alleles, showing 4G allele (p= 1.524e-10; OR= 0.262) has decreasing effect and 5G allele (p= 1.524e-10; OR= 3.822) has increasing effect in ectopic pregnancy.
Conclusion: According to the findings, 5G allele and 4G/5G and 5G/5G genotypes have increasing effect, 4G allele and 4G/4G genotype have decreasing effect in ectopic pregnancy. So, we could consider 5G allele as a risk factor of ectopic pregnancy in this study.
Background: Ectopic pregnancy is a type of pregnancy in which implantation of zygote occurs out of the uterine cavity. One of the most important problems is bleeding. On the other hand, Plasminogen Activator Inhibitor-1(PAI-1) gene is one of the involved factors in unsuccessful pregnancies, and 4G/5G polymorphism is the most common changes of this gene. So, it is important to study the prevalence of these changes in this gene in women with ectopic pregnancy.
Materials and Methods: In this case-control study, 100 Iranian women with ectopic pregnancy and 101 Iranian women with the normal pregnancy were selected. After blood sampling, ARMS PCR method has been used for detection 4G/5G polymorphism and data were analyzed by statistical analysis.
Results: In this study, 4G allele with 70.79% prevalence and 5G allele with 63.5% prevalence are the most common alleles for the control and case group, respectively. 4G/4G and 4G/5G genotypes in the control group and 4G/5G and 5G/5G genotypes in the case group are prevalent. An Armitage test found p<0.05 for both alleles, showing 4G allele (p= 1.524e-10; OR= 0.262) has decreasing effect and 5G allele (p= 1.524e-10; OR= 3.822) has increasing effect in ectopic pregnancy.
Conclusion: According to the findings, 5G allele and 4G/5G and 5G/5G genotypes have increasing effect, 4G allele and 4G/4G genotype have decreasing effect in ectopic pregnancy. So, we could consider 5G allele as a risk factor of ectopic pregnancy in this study.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/06/12 | Accepted: 2017/09/6
Received: 2017/06/12 | Accepted: 2017/09/6
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
