Volume 21, Issue 4 (8-2018)                   J Arak Uni Med Sci 2018, 21(4): 86-97 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mombeyni A, Bahmanzade M, Sarami A, Changizi-Ashtiyani S, Parastesh M. The Effect of Increasing Resistance Training on Testicular Oxidative Stress and Quality of Spermatogenesis in Male Rats. J Arak Uni Med Sci 2018; 21 (4) :86-97
URL: http://jams.arakmu.ac.ir/article-1-5197-en.html
1- Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran. , amin4mm64@yahoo.com
2- Department of Sport Physiology and Pathology, Faculty of Sport Sciences, Arak University, Arak, Iran.
3- Department of Medical Physiology, Arak University of Medical Sciences, Arak, Iran.
Abstract:   (2546 Views)
Background and Aim: Following physical activity in various tissues of the body, oxidative stress occurs and affects the antioxidant capacity and consequently the fertility of men negatively. The aim of this study was to investigate the effects of resistance training on oxidative stress and spermatogenesis quality.
Materials and Methods: 20 male rats of Sprague Dawley (weight 200-250, 4 months old) were randomly divided into two groups of resistance training and control group (n = 10). The exercise program included climbing a ladder by hanging the load to the tail for 8 weeks (5 days a week, 3 times 4 repetitions). During the program, the load gradually was increased, and in the last week, it reached to 200% of the animal body weight. After intervention, the mice were anesthetized in separate groups and testicular tissue was isolated to evaluate Malondialdehyde index as an oxidative stress marker and to determine antioxidant capacity by Frap method. Fixation, processing, cutting of testicular tissue and eventually coloring steps were examined by Hematoxylin and Eosin (H & E). The spermatogenesisqualitywas calculated based on Johnson method. The data were analyzed by independent t-test was used and significance level was considered p <0.05.
Findings: Increased Malondialdehyde levels were observed in the treatment group compared to control group (p =0.01). In the training group, the spermatogenesis quality was lower (p =0.013), the antioxidant capacity in the training group was significantly higher than the control group (p=0.83) and there isn’t any significant difference.
Conclusion: The findings indicate thatdue to increased oxidative stress, increasing resistance training may have negative effects on the quality of spermatogenesis and men's fertility.
Full-Text [PDF 2118 kb]   (1800 Downloads)    
Type of Study: Original Atricle | Subject: General
Received: 2017/06/25 | Accepted: 2018/07/2

References
1. Cakir-Atabek H, Demir S, PinarbaŞili RD, Gündüz N. Effects of different resistance training intensity on indices of oxidative stress. J Strength Cond Res. 2010; 24(9):2491-7. doi: 10.1519/JSC.0b013e3181ddb111.
2. Khosrowbeygi A. The role of oxidative stress in male infertility: A review. Arak Medical University Journal (AMUJ). 2013; 15(68): 94-103.
3. Ribeiro AS1, Deminice R, Schoenfeld BJ, Tomeleri CM2, Padilha CS2, Venturini D4, Barbosa DS4, Sardinha LB5, Cyrino ES2. Effect of Resistance Training Systems on Oxidative Stress in Older Women, Int J Sport NutrExercMetab. 2017; 19:1-23. doi: 10.1123/ijsnem.2016-0322.
4. Zini A, San Gabriel M, Baazeem A. Antioxidants and sperm DNA damage: a clinical perspective. Journal of assisted reproduction and genetics. 2009; 26(8):427-32.
5. Fanaeei H, Azizi Y, Khayat S. The role of stress oxidative in infertility male. A review. Fasa Medical University Journal (FMUJ). 2014; 2: 93-103.
6. Saremi A, Shavandi N, Changizi Ashtiani S, Bahmanzadeh M: The effects of progressive resistance training on spermatogenesis and reproductive hormones in male rats Journal of Sport inBiomotor Sciences. 2013; 7(1):14-21.
7. Stefan S. du Plessis, Anthony Kashou, Diana Vaamonde and Ashok Agarwal. Is There a link between Exercise and Male Factor Infertility? The Open Reproductive Science Journal. 2011; 3: 105-113.
8. Delavar R, Mogharnasi M, Khoobkhahi N, The Effects of Combined Training on Oxidative Stress and Antioxidant Defense Indicators, Int. J Basic Sci Med. 2017; 2(1):29-32.
9. Jóźków P, Rossato M. The Impact of Intense Exercise on Semen Quality.Am J Mens Health. 2017; 11(3):654-662.
10. Tartibian B, Maleki BH. The effects of honey supplementation on seminal plasma cytokines, oxidative stress biomarkers and antioxidants during 8 weeks of intensive cycling training. J Androl. 2012; 33(3): 449-461.
11. Safarinejad MR, Azma K, Kolahi AA. The effects of intensive, long-term treadmill running on reproductive hormones, hypothalamus-pituitary-testis axis, and semen quality: a randomized controlled study. J Endocrinol. 2009; 200(3): 259-71
12. Nirupama M, Devaki M, Nirupama R, Yajurvedi HN. "Chronic intermittent stress-induced alterations in the spermatogenesis and antioxidant status of the testis are irreversible in albino rat". J Physiol Biochem. 2013; 69(1): 59-68.
13. Gebreegziabher, Y., Marcos, E., McKinon, W., & Rogers, G. Sperm characteristics of endurance trained cyclists. International Journal of Sports Medicine. 2004; 25(4): 247-251.
14. Hoffman, JR., Im, J, Kang, J, Maresh, CM, Kraemer, WJ, French, D, Nioka, S, Kime, R, Rundell, KW, Ratamess, NA, Faigenbaum, AD, and Chance, B. Comparison of low- and high-intensity resistance exercise on lipid peroxidation: Role of muscle oxygenation. J Strength Cond Res. 2007; 21: 118–122.
15. Banaeifar A., Gorzi A., Hedayati M., Nabiollahi Z, Rahmani-Moghaddam N, Khantan M. Effect of an 8-week resistance training program on acetylcholine esterase activity in rat muscle. Feyz. 2012; 15: 316-21.
16. Sukho L. and Roger P. Farrar. Resistance Training Induces Muscle-Specific Changes in Muscle Mass and Function in Rat. An International Electronic Journal. 2003; 6 (2): 73-87.
17. Johnsen, S. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones. 1970; 1(1): 2-25.
18. Ohkawa, H., Ohishi, N., &Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979; 95(2): 351-358.
19. Hajizadeh Maleki B, Tartibian B, Frank C. Mooren, Yaghoob Nezhad F, Yaseri M. Saffron supplementation ameliorates oxidative damage to sperm DNA following a 16-week low-to-intensive cycling training in male road cyclists Journal of Functional Foods. 2016; 21:153-166.
20. Manna I, Jana K, Samanta PK. Effect of intensive exercise-induced testicular gametogenic and steroidogenic disorders in mature male Wistar strain rats: a correlative approach to oxidative stress. ActaPhysiolScand. 2003; 178: 33-40.
21. Saremi A, Changizi Ashtiyani S, Shavandi N, Mombeyni A. Effect of different intensive training on spermatogenesis and reproductive system hormones in male obese rats. Journal of sport biosciences. 2013; 17: 81-94.
22. Park SY, Kwak YS. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. J Exerc Rehabil. 2016; 12(2):113-118.
23. Torma F1, Koltai E1, Nagy E1, Ziaaldini MM1, Posa A2, Koch LG3, Britton SL3, Boldogh I4, Radak Z1. Exercise Increases Markers of Spermatogenesis in Rats Selectively Bred for Low Running Capacity. PLoS One. 2014; 9(12): e114075.
24. Bloomer, RJ, Fry, AC, Falvo, MJ, and Moore, CA. Protein carbonyls are acutely elevated following single set anaerobic exercise inresistance trained men. J Sci Med Sport. 2007:10: 411–417.
25. Hajizadeh Maleki B, Tartibian B, Eghbali M, Asri-Rezaei S. Comparison of seminal oxidants and antioxidants in subjects with different levels of physical fitness. Andrology. 2013; 1(4):607-14.
26. Saremi A, Mombeini A. Influence of swimming exercise training on semen quality and oxidative stress status of the testis in obese male rats Journal of Practical Studies of Biosciences in Sport. 2015/2016; 65-73.
27. Dantas FF, Brasileiro-Santos Mdo S, Batista RM, et al. Effect of strength training on oxidative stress and the correlation of the same with forearm vasodilatation and blood pressure of hypertensive elderly women: a randomized clinical trial. PLoS One. 2016; 11(8): e0161178.
28. Azizbeigi K, Azarbayjani MA, Peeri M, Agha-alinejad H, Stannard S. The effect of progressive resistance training on oxidative stress and antioxidant enzyme activity in erythrocytes in untrained men. Int J Sport NutrExercMetab. 2013; 23(3):230-8.
29. Azarbayjani MA. Azizbeigi K, Atashak S, Stannard SR. Effect of moderate and high resistance training intensity on indices of inflammatory and oxidative stress. Res Sports Med. 2015; 23(1):73-87.
30. Parastesh M, Heidarianpour A, Bayat. M, Saremi A. Effects of Resistance Training on Reproductive Hormones and sperm parameters in Type 2 Diabetes Rats. Arak Medical University Journal (AMUJ). 2016; 19(113): 26-36.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb