Volume 20, Issue 7 (10-2017)
J Arak Uni Med Sci 2017, 20(7): 65-76 |
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Alinaghipour A, Tavassoli M, Seyed Hosseini E, Ardjmand A. Effects of Ethanol Preconditioning on Pentylenetetrazole-induced Memory Impairment and Expression of NMDA Receptor NR1 Subunit mRNA in Rat. J Arak Uni Med Sci 2017; 20 (7) :65-76
URL: http://jams.arakmu.ac.ir/article-1-5225-en.html
URL: http://jams.arakmu.ac.ir/article-1-5225-en.html
1- Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran
2- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
3- Ph.D of Neuroscience, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran ,ardjmand_ab@kaums.ac.ir
2- Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
3- Ph.D of Neuroscience, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran ,
Abstract: (4549 Views)
Abstract
Background: Neuronal damage following seizures and epilepsy is one of the main causes of disabilities and mortality worldwide. In recent years, preconditioning has been introduced as a novel strategy for the prevention of brain damage. Preconditioning is a phenomenon in which a minor noxious stimulus protects from a subsequent more severe insult. The aim of present study was to examine the effect of ethanol (Eth) preconditioning on pentylenetetrazole (PTZ)-induced impairment memory in the inhibitory avoidance model.
Material and Methods: This study was carried out on 45 adult male Wistar rats (180-200 g). Animals were assigned into five groups: Control, Eth 0.25, Eth 0.5, PTZ and Eth (0.5) +PTZ (n=9, for all groups). Eth-preconditioning was induced 6 days before the injection of PTZ. The animals were tested in a single trial step-through inhibitory test in two sessions (train and test). Then locomotor activity of rats was recorded in the open-field apparatus and NR1 mRNA expression in the hippocampus was measured by real-time PCR technique.
Results: One-way ANOVA revealed that the Ethanol preconditioning did not impair inhibitory memory. Further, post-test analyses showed that Ethanol preconditioning significantly prevented from PTZ-induced memory impairment, and increased NR1 subunit mRNA expression in PTZ-induced memory impairment group. In addition, one-way ANOVA for the locomotor activity showed no significant difference between the groups.
Conclusion: Our results showed that a pre-conditioning treatment with Ethanol
(0.5g/kg/day), 6 days before PTZ-induced memory impairment may provide a kind of neuroprotection in rats.
Background: Neuronal damage following seizures and epilepsy is one of the main causes of disabilities and mortality worldwide. In recent years, preconditioning has been introduced as a novel strategy for the prevention of brain damage. Preconditioning is a phenomenon in which a minor noxious stimulus protects from a subsequent more severe insult. The aim of present study was to examine the effect of ethanol (Eth) preconditioning on pentylenetetrazole (PTZ)-induced impairment memory in the inhibitory avoidance model.
Material and Methods: This study was carried out on 45 adult male Wistar rats (180-200 g). Animals were assigned into five groups: Control, Eth 0.25, Eth 0.5, PTZ and Eth (0.5) +PTZ (n=9, for all groups). Eth-preconditioning was induced 6 days before the injection of PTZ. The animals were tested in a single trial step-through inhibitory test in two sessions (train and test). Then locomotor activity of rats was recorded in the open-field apparatus and NR1 mRNA expression in the hippocampus was measured by real-time PCR technique.
Results: One-way ANOVA revealed that the Ethanol preconditioning did not impair inhibitory memory. Further, post-test analyses showed that Ethanol preconditioning significantly prevented from PTZ-induced memory impairment, and increased NR1 subunit mRNA expression in PTZ-induced memory impairment group. In addition, one-way ANOVA for the locomotor activity showed no significant difference between the groups.
Conclusion: Our results showed that a pre-conditioning treatment with Ethanol
(0.5g/kg/day), 6 days before PTZ-induced memory impairment may provide a kind of neuroprotection in rats.
Keywords: Epilepsy, Ethanol, Inhibitory avoidance Memory, Preconditioning, Pentylenetetrazole, Seizure
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/07/15 | Accepted: 2017/08/2
Received: 2017/07/15 | Accepted: 2017/08/2
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