Volume 21, Issue 1 (4-2018)
J Arak Uni Med Sci 2018, 21(1): 1-10 |
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Biglari S, Gaeini A A, Kordi M R, Ghardashi Afousi A. The Effect of 8 Weeks High-intensity Interval Training on Myostatin and Follistatin Gene Expression in Gastrocnemius Muscle of the Rats. J Arak Uni Med Sci 2018; 21 (1) :1-10
URL: http://jams.arakmu.ac.ir/article-1-5477-en.html
URL: http://jams.arakmu.ac.ir/article-1-5477-en.html
1- Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran , s.biglari.physiology@gmail.com
2- Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran
3- Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran.
2- Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran
3- Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran.
Abstract: (7336 Views)
Abstract
Background: The purpose of the present study is to investigate the effect of 8 weeks High-intensity Interval Training (HIIT) on the expression of two muscle growth regulating genes (myostatin and follistatin) in gastrocnemius muscle of healthy male rats.
Materials and Methods: 16 male Wistar rats were randomly divided into two groups in the same number: control and HIIT. HIIT program was underwent 40 min each session, three sessions in a week for eight weeks. Each exercise training session consisted of 5 min warm-up and cool-down at 40-50 % VO2max, 30 min interval running including 4 min high-intensity (85-90% VO2max) and 2 min active recovery (at 50-60% VO2max). Rats in control group did not do any exercise training program. 48 h after the last training session, rats` gastrocnemius muscle was extracted and the expression of myostatin and follistatin genes was determined by Real Time-PCR. For statistical data analysis, independent t-test was used.
Results: The expression of myostatin was significantly reduced 68% in HIIT group in comparison with the control group (p<0.05). However, there was no significant difference in follistatin expression in HIIT group compared to the control group (p>0.05). Gastrocnemius muscle weight was significantly increased 23% in the HIIT group compared to the control group (p<0.05).
Conclusion: Results indicated that HIIT lead to significant reduction in the expression of myostatin gene and increase in the weight of gastrocnemius muscle in rats.
Background: The purpose of the present study is to investigate the effect of 8 weeks High-intensity Interval Training (HIIT) on the expression of two muscle growth regulating genes (myostatin and follistatin) in gastrocnemius muscle of healthy male rats.
Materials and Methods: 16 male Wistar rats were randomly divided into two groups in the same number: control and HIIT. HIIT program was underwent 40 min each session, three sessions in a week for eight weeks. Each exercise training session consisted of 5 min warm-up and cool-down at 40-50 % VO2max, 30 min interval running including 4 min high-intensity (85-90% VO2max) and 2 min active recovery (at 50-60% VO2max). Rats in control group did not do any exercise training program. 48 h after the last training session, rats` gastrocnemius muscle was extracted and the expression of myostatin and follistatin genes was determined by Real Time-PCR. For statistical data analysis, independent t-test was used.
Results: The expression of myostatin was significantly reduced 68% in HIIT group in comparison with the control group (p<0.05). However, there was no significant difference in follistatin expression in HIIT group compared to the control group (p>0.05). Gastrocnemius muscle weight was significantly increased 23% in the HIIT group compared to the control group (p<0.05).
Conclusion: Results indicated that HIIT lead to significant reduction in the expression of myostatin gene and increase in the weight of gastrocnemius muscle in rats.
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