Volume 21, Issue 4 (8-2018)
J Arak Uni Med Sci 2018, 21(4): 48-57 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Abdalan S, Baghbani-Arani F, Sadat Shandiz S A. Evaluation of Anticancer Effect of Aqueous and Hydroalcoholic Extracts of Quercusin Fectoria Leaf against Colon Cancer HT29 Cell Line. J Arak Uni Med Sci 2018; 21 (4) :48-57
URL: http://jams.arakmu.ac.ir/article-1-5491-en.html
URL: http://jams.arakmu.ac.ir/article-1-5491-en.html
1- Department of Genetics and Biotechnology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.
2- Department of Genetics and Biotechnology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran. ,fbaghbani@iauvaramin.ac.ir
3- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Genetics and Biotechnology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran. ,
3- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Abstract: (4319 Views)
| Background and Aim: In traditional medicine and previous studies, Quercus infectoria plants have been suggested as a cancer treatment. The aim of the current study was to investigate the cytotoxic effect of aqueous and hydro alcoholic extracts of Quercus infectoria leaf against colon cancer HT29 cell line and to evaluate the Bax and Bcl-2 gene expression in treated cells. Materials and Methods: In this study, aqueoFus and hydro alcoholic extracts of Quercus infectoria leaf were prepared. Then, the HT29 and HEK293 cell lines were treated by various concentrations of extracts for 24 hours and the cytotoxicity effect of extracts was estimated by colorimetric MTT (methyl thiazolyl tetrazolium) assay. Finally, the pro-apoptotic Bax and anti-apoptotic Bcl-2 gene expression in treated cells compared to GAPDH reference gene expression was evaluated using real time PCR technique. Findings: According to the MTT results, the cytotoxic activity of aqueous extract has dose-dependent manner against both cell lines, therefore, the Bax and Bcl-2 gene expression levels in treated cells by aqueous extract were changed 2.8 (p˂0.05) and 2.2-fold (p˃0.05) compared to reference gene, respectively. Conclusion: According to the results, it seems that the aqueous extract of Quercus infectoria leaf has the potential for apoptosis induction in colon cancer HT29 cell line and based on more studies, it can be used as a colon cancer treatment. |
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/12/3 | Accepted: 2018/04/22
Received: 2017/12/3 | Accepted: 2018/04/22
References
1. Siegel R, DeSantis C, Jemal A. Colorectal cancer statistics, 2014.CA Cancer J Clin 2014; 64(2):104-17.
2. Green DR, Evan GI. A Matter of Life and Death. Cancer Cell. 2002; 1(1):19-30.
3. BasriD, Fan SH. The potential of aqueous and acetone extract of galls of quercus infectoria as antibacterial agents. Indian J Pharmacol 2005; 37: 26-29.
4. Kaur G, Hamid H, Ali A, Alam MS, Athar M. Antiinflammatory evaluation of alcoholic extract of galls of Quercus infectoria. J Ethnopharmacol 2004; 90(2-3):285-92.
5. Umachigi SP, Jayaveera KN, Kumar CA, Kumar GS, Kumar DK. Studies on Wound Healing Properties of Quercus infectoria. Trop J Pharm Res. 2008; 7 (1): 913-919.
6. Lim TK. Edible medicinal and non-medicinal plants: New York, NY, USA: Springer; 2012.
7. Yin C, Knudson CM, Korsmeyer SJ, Van Dyke T. Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Nature. 1997; 385(6617):637-40.
8. Yoo KC, Yoon CH, Kwon D, Hyun KH, Woo SJ, Kim RK, Lim EJ, Suh Y, Kim MJ, Yoon TH, Lee SJ. Titanium dioxide induces apoptotic cell death through reactive oxygen species-mediated Fas upregulation and Bax activation. Int J Nanomedicine. 2012; 7:1203-14.
9. Sinicrope FA, Cleary KR, Stephens LC, Lee JJ, Levin B. bcl-2 and p53 oncoprotein expression during colorectal tumorigenesis. Cancer Res. 1995; 15; 55(2):237-41.
10. Trusheva B, Trunkova D, Bankova V. Different extraction methods of biologically active components from propolis: a preliminary study. Chem Cent J. 2007; 1(1):13.
11. Hasmah A, Nurazila Z, Chow CY, Rina R, Rafiquzzaman M. Cytotoxic effects of Quercus infectoria extracts towards cervical (Hela) and Ovarian (Caov-3) cancer cell lines. Health and the Environment Journal. 2010; 1(2):17-23.
12. Sak K. Chemotherapy and dietary phytochemical agents. Chemother Res Pract. 2012; 20; 2012.
13. Shariatifar N, Fathabad AE, Khaniki GJ, Nasrabadi HG. Evaluation of the antibacterial activity of essential oil and aqueous and ethanolic extracts of Quercus infectoria leaves on food-borne pathogenic bacteria. Int J Pharm Sci Res. 2014; 5(10):709-13.
14. Roshni P. Ramesh. KG. Anticancer Activity of Ac-etone Extract of Quercus Infectoria Olivier Fagaceae in 1, 2 Dimethyl Hy-drazine Induced Colon Cancer. Int J Cancer Stud Res. 2013; 2(1):13-7.
15. Yarani R, Mansouri K, Mohammadi-Motlagh HR, Mahnam A, Emami Aleagha MS. In vitro Inhibition of Angiogenesis by Hydroalcoholic Extract of Oak (Quercus infectoria) a corn Shell via Suppressing VEGF, MMP-2, and MMP-9 Secretion. Pharm Biol. 2013; 51(3):361-8.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
