Volume 21, Issue 3 (6-2018)                   J Arak Uni Med Sci 2018, 21(3): 14-23 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Esmaeili S, Minasian V, Bayat M, Karami H. Effect of Exercise Training on Vascular Endothelial Growth Factor and Its Receptor Gene Expression in Cardiac Tissue of Type 2 Diabetic Rats. J Arak Uni Med Sci 2018; 21 (3) :14-23
URL: http://jams.arakmu.ac.ir/article-1-5632-en.html
1- Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
2- Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran. , vazgenmin@yahoo.com
3- Department of Molecular Medicine and Biotechnology, Faculty of Medical Sciences, Arak University of Medical Sciences, Arak, Iran.
Abstract:   (2615 Views)
Background and Aim: Type 2 diabetes is one of the effective and inhibiting factors in controlling blood glucose and vascular disorders. The aim of this study was to investigate the changes in the gene expression of vascular endothelial growth factor and its type 1 receptor in cardiac tissue of type 2 diabetic rats following three different training methods.
Materials and Methods: In this study, 60 rats were randomly divided into 5 equal groups: healthy control, diabetic control, and diabetic groups with endurance, resistance, and combined exercise training. Type 2 diabetes mellitus was induced by intraperitoneal injection of streptozotocin and exercises were performed 5 sessions per week for 8 weeks. Evaluation of the levels of gene expression of vascular endothelial growth factor and its receptor 1 was performed by RT-qPCR.
Findings: The results showed a significant reduction in the expression of vascular endothelial growth factor in diabetic control, endurance training and resistance training groups, as well as a significant increase in expression of its receptor in diabetic control group and all training groups compared to healthy control group (p <0.001). Comparisons with the diabetic control group showed that in all training groups, the vascular endothelial growth factor gene expression increased, but in the its receptor 1 it was significantly decreased (p <0.001).
Conclusion: The findings suggest that different training exercises are effective in improving angiogenesis, but combined exercises have a certain superiority compared to other exercises.

 
Full-Text [PDF 504 kb]   (942 Downloads)    
Type of Study: Original Atricle | Subject: Cardiology
Received: 2018/02/16 | Accepted: 2018/05/9

References
1. DeFronzo RA, Ferrannini E, Groop L, Henry RR, Herman WH, Holst JJ, et al. Type 2 diabetes mellitus. Nature Reviews Disease Primers. 2015; 1:15019.
2. Dagogo-Jack S. Diabetes mellitus in developing countries and underserved communities. 1nd ed. Switzerland: Springer. 2017; 52-54.
3. DeFronzo R. Insulin resistance, lipotoxicity, type 2 diabetes and atherosclerosis: the missing links. The Claude Bernard Lecture 2009. Diabetologia. 2010; 53(7):1270-87.
4. Al-Harris ES, Al-Janabi AA, Al-Toriahi KM, Yasseen AA. Over expression of vascular endothelial growth factor in correlation to Ki-67, grade, and stage of breast cancer. Saudi medical journal. 2008; 29(8):1099-104.
5. Smith GA, Fearnley GW, Harrison MA, Tomlinson DC, Wheatcroft SB, Ponnambalam S. Vascular endothelial growth factors: multitasking functionality in metabolism, health and disease. Journal of inherited metabolic disease. 2015; 38(4):753-63.
6. Waltenberger J. Impaired collateral vessel development in diabetes: potential cellular mechanisms and therapeutic implications. Cardiovascular research. 2001; 49(3):554-60.
7. Gao Y, Zhao Y, Pan J, Yang L, Huang T, Feng X, et al. Treadmill exercise promotes angiogenesis in the ischemic penumbra of rat brains through caveolin-1/VEGF signaling pathways. Brain Res. 2014; 1585:83-90.
8. Shibuya M. Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling in angiogenesis: a crucial target for anti-and pro-angiogenic therapies. Genes & cancer. 2011; 2(12):1097-105.
9. Simons M, Gordon E, Claesson-Welsh L. Mechanisms and regulation of endothelial VEGF receptor signalling. Nature Reviews Molecular Cell Biology. 2016; 17(10):611-25.
10. Takahashi H, Shibuya M. The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions. Clinical science. 2005; 109(3):227-41.
11. Thiagarajan H, Thiyagamoorthy U, Shanmugham I, Nandagopal GD, Kaliyaperumal A. Angiogenic growth factors in myocardial infarction: a critical appraisal. Heart failure reviews. 2017; 22(6):665-83.
12. Erekat NS, Al-Jarrah MD, Al Khatib AJ. Treadmill exercise training improves vascular endothelial growth Factor expression in the cardiac muscle of type I diabetic rats. Cardiology Research. 2014; 5(1):23.
13. Shekarchizadeh P, Khazaei M, Gharakhanlou R, Karimian J, Safarzadeh AR. [The Effects of Resistance Training on Plasma Angiogenic Factors in Normal Rats]. Journal of isfahan medical school. 2012; 30(176):1-9.
14. Nourshahi M, Babaei A, Bigdeli MR, Ghasemi BM. The effect of six weeks of resistance training on tumor tissue VEGF and endostatin in mice with breast cancer. Sport Biosciences. 2013; 5(17):27-46.
15. Fernandez‐Gonzalo R, Lundberg TR, Tesch PA. Acute molecular responses in untrained and trained muscle subjected to aerobic and resistance exercise training versus resistance training alone. Acta Physiologica. 2013; 209(4):283-94.
16. Francois ME, Little JP. Effectiveness and safety of high-intensity interval training in patients with type 2 diabetes. Diabetes Spectrum. 2015; 28(1):39-44.
17. Punitha I, Rajendran K, Shirwaikar A, Shirwaikar A. Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin–nicotinamide induced diabetic rats. Evidence-Based Complementary and Alternative Medicine. 2005; 2(3):375-81.
18. Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiology & behavior. 2015; 147:78-83.
19. Kim H-J, So B, Son JS, Song HS, Oh SL, Seong JK, et al. Resistance training inhibits the elevation of skeletal muscle derived-BDNF level concomitant with improvement of muscle strength in zucker diabetic rat. Journal of exercise nutrition & biochemistry. 2015; 19(4):281.
20. Kim J-y, Choi MJ, So B, Kim H-j, Seong JK, Song W. The Preventive Effects of 8 Weeks of Resistance Training on Glucose Tolerance and Muscle Fiber Type Composition in Zucker Rats. Diabetes & metabolism journal. 2015; 39(5):424-33.
21. Hazarika S, Dokun AO, Li Y, Popel AS, Kontos CD, Annex BH. Impaired angiogenesis after hindlimb ischemia in type 2 diabetes mellitus: differential regulation of vascular endothelial growth factor receptor 1 and soluble vascular endothelial growth factor receptor 1. Circulation research. 2007; 101(9):948-56.
22. Gao L, Yu D-m. Molecular mechanism of limbs' postischemic revascularization improved by perindopril in diabetic rats. Chinese medical journal. 2008; 121(21):2129-33.
23. Khazaei M, Fallahzadeh AR, Sharifi MR, Afsharmoghaddam N, Javanmard SH, Salehi E. Effects of diabetes on myocardial capillary density and serum angiogenesis biomarkers in male rats. Clinics. 2011; 66(8):1419-24.
24. Motahari Rad M, Attarzadeh Hosseini SR. Response of vascular endothelial growth factor and endostatin to a session activity before and after a period of L-arginine supplementation in active men. AMUJ. 2017; 20(119):78-88.
25. Rodrigues B, Jorge L, Mostarda CT, Rosa KT, Medeiros A, Malfitano C, et al. Aerobic exercise training delays cardiac dysfunction and improves autonomic control of circulation in diabetic rats undergoing myocardial infarction. Journal of cardiac failure. 2012; 18(9):734-44.
26. Reed JL, Pipe AL. Practical approaches to prescribing physical activity and monitoring exercise intensity. Canadian Journal of Cardiology. 2016; 32(4):514-22.
27. Olver TD, Ferguson BS, Laughlin MH. Chapter Ten-Molecular Mechanisms for Exercise Training-Induced Changes in Vascular Structure and Function: Skeletal Muscle, Cardiac Muscle, and the Brain. Progress in molecular biology and translational science. 2015; 135: 227-57.
28. Higashi Y, Murohara T. Therapeutic Angiogenesis. 1nd ed. Singapore: Springer.2017; 229.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb