Volume 21, Issue 5 (10-2018)
J Arak Uni Med Sci 2018, 21(5): 98-109 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Motamedi H, Dehbashi S, Tahmasebi H, Arabestani M R. Survey the Role and Effect of Some Antibiotic Resistance in the Spread of Pathogenic Strains of Staphylococcus aureus in Different Clinical Specimens
. J Arak Uni Med Sci 2018; 21 (5) :98-109
URL: http://jams.arakmu.ac.ir/article-1-5647-en.html
URL: http://jams.arakmu.ac.ir/article-1-5647-en.html
1- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
3- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. ,mohammad.arabestani@gmail.com
2- Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
3- Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. ,
Abstract: (2774 Views)
Background and Aim: Staphylococcus aureus(S.aureus) has many pathogens. Antibiotic resistance may increase the invasion of this bacterium. The aim of this study was to determine the role and effect of some antibiotic resistance in the spread of pathogenic strains of S.aureus in different clinical specimens.
Materials and Methods: 95 clinical isolates of S.aureus were collected from different clinical specimens. Antibiotic resistance pattern was determined by Disc diffusion method (Kirby-Bauer) for 6 different classes. Identification of adhesion agent genes in isolated isolates was performed using Multiplex-PCR and specific primers. For analysis of the results, GraphPad Prism version 6 and ꭕ2 statistical sampling was used. p≤0.05 was considered significant.
Findings: Of 95 isolates of S.aureus, 29 isolates (30.52%) were resistant to methicillin, 12 isolates (12.63%), resistant to clindamycin, 48 isolates (50.52%), resistant to gatyfloxacin, 88 (92.63%) isolates resistant to gentamicin, 57 (60%) isolates resistant to erythromycin and 79 isolates (83.15%) were resistant to tetracycline. fnbA genes were isolated in 14 isolates (14.73%), fnbB in 29 isolates (30.52%), fib in 21 isolates (22.10%), clfA in 17 isolates (17.89%) and clfB in 19 isolates (20%). There was a significant correlation between resistance to macular antibiotics, tetracycline, beta-lactam, lacosamide, aminoglycoside and pathogens.
Conclusion: The adhesion factors in S.aureus possibly cause some structural changes and cause resistance to various antibiotic classes.
Materials and Methods: 95 clinical isolates of S.aureus were collected from different clinical specimens. Antibiotic resistance pattern was determined by Disc diffusion method (Kirby-Bauer) for 6 different classes. Identification of adhesion agent genes in isolated isolates was performed using Multiplex-PCR and specific primers. For analysis of the results, GraphPad Prism version 6 and ꭕ2 statistical sampling was used. p≤0.05 was considered significant.
Findings: Of 95 isolates of S.aureus, 29 isolates (30.52%) were resistant to methicillin, 12 isolates (12.63%), resistant to clindamycin, 48 isolates (50.52%), resistant to gatyfloxacin, 88 (92.63%) isolates resistant to gentamicin, 57 (60%) isolates resistant to erythromycin and 79 isolates (83.15%) were resistant to tetracycline. fnbA genes were isolated in 14 isolates (14.73%), fnbB in 29 isolates (30.52%), fib in 21 isolates (22.10%), clfA in 17 isolates (17.89%) and clfB in 19 isolates (20%). There was a significant correlation between resistance to macular antibiotics, tetracycline, beta-lactam, lacosamide, aminoglycoside and pathogens.
Conclusion: The adhesion factors in S.aureus possibly cause some structural changes and cause resistance to various antibiotic classes.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2018/02/26 | Accepted: 2018/04/18
Received: 2018/02/26 | Accepted: 2018/04/18
References
1. Tahmasebi H, Zeiyni B, Dehbashi S, Motamedi H, Vafaeifar M, Keramat F, et al. The Study of blaZ and mecA Gene Expression in Methicillin-Resistant Staphylococcus aureus Strains and the Relationship between the Gene Expression Patterns. Journal of Isfahan Medical School. 2017; 443 (35):1062-67.
2. Adabi J, Shahraki Zahedani S, Bokaeian M, Tahmasebi H. An Investigation of the Prevalence of AmpC-producing Pseudomonas aeruginosa in Clinical Samples in Zahedan City, Iran. Qom Univ Med Sci J. 2017; 11(4):61-71.
3. Stevenson KB, Wang SH. Understanding the molecular pathogenesis of methicillin-resistant Staphylococcus aureus. J Infect Dis. 2014; 209(4):488-90.
4. Mollaei M, Rashki A. The Prevalence of Adhesive Surface Encoding Genes in Staphylococcus Aureus Isolated from Hospitalized Patients in Zabol-Iran by Multiplex PCR. Journal of Fasa University of Medical Sciences. 2016; 6(3):296-302.
5. McAleese FM, Foster TJ. Analysis of mutations in the Staphylococcus aureus clfB promoter leading to increased expression. Microbiology. 2003; 149(Pt 1):99-109.
6. Atshan SS, Nor Shamsudin M, Sekawi Z, Lung LTT, Hamat RA, Karunanidhi A, et al. Prevalence of Adhesion and Regulation of Biofilm-Related Genes in Different Clones of Staphylococcus aureus. Journal of Biomedicine and Biotechnology. 2012; 2012:10.
7. Nourbakhsh F, Namvar AE. Detection of genes involved in biofilm formation in Staphylococcus aureus isolates. GMS Hygiene and Infection Control. 2016; 11:Doc07.
8. William da Fonseca Batistão D, Amaral de Campos P, Caroline Camilo N, Royer S, Fuga Araújo B, Spirandelli Carvalho Naves K, et al. Biofilm formation of Brazilian meticillin-resistant Staphylococcus aureus strains: prevalence of biofilm determinants and clonal profiles. Journal of medical microbiology. 2016; 65(4):286-97.
9. Motamedi H, Asghari B, Tahmasebi H, Arabestani MR. Adhesion Factors and Association with Antibiotic Resistance among Clinical Isolates of Staphylococcus aureus. 2017. Iranian journal of Medical Microbiology. 2017; 11(3):27-36.
10. Urazgil'deev ZI, Shakina Iu G, Vaneeva NP, Iastrebova NE. [Antibodies to Staphylococcus aureus teichoic acids in the pathogenesis of chronic osteomyelitis]. Zh Mikrobiol Epidemiol Immunobiol. 1987(9):61-4.
11. Oeding P, Grov A. Antigen preparations from Staphylococcus aureus. Contrib Microbiol Immunol. 1973; 1:77-82.
12. Maeda M, Shoji H, Shirakura T, Takuma T, Ugajin K, Fukuchi K, et al. Analysis of Staphylococcal Toxins and Clinical Outcomes of Methicillin-Resistant Staphylococcus aureus Bacteremia. Biological & pharmaceutical bulletin. 2016; 39(7):1195-200.
13. Vafaee Mehr M, Alikhani M, Tahmasebi H, Arabestani M. Identification and Determination of the Relationship between ccr Alleles and Antibiotic Resistance in Clinical Isolates of Methicillin Resistant Staphylococcus aureus. Journal of Babol University Of Medical Sciences. 2017; 19(12):28-35.
14. Huesca M, Peralta R, Sauder DN, Simor AE, McGavin MJ. Adhesion and Virulence Properties of Epidemic Canadian Methicillin-Resistant Staphylococcus aureus Strain 1: Identification of Novel Adhesion Functions Associated with Plasmin-Sensitive Surface Protein. The Journal of Infectious Diseases. 2002; 185(9):1285-96.
15. Nemati M, Hermans K, Devriese LA, Maes D, Haesebrouck F. Screening of genes encoding adhesion factors and biofilm formation in Staphylococcus aureus isolates from poultry. Avian Pathology. 2009; 38(6):513-7.
16. Dakheel KH, Abdul Rahim R, Neela VK, Al-Obaidi JR, Hun TG, Yusoff K. Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. BioMed research international. 2016; 2016:14.
17. Balasubramanian D, Ohneck EA, Chapman J, Weiss A, Kim MK, Reyes-Robles T, et al. Staphylococcus aureus Coordinates Leukocidin Expression and Pathogenesis by Sensing Metabolic Fluxes via RpiRc. mBio. 2016; 7(3): e00818-16.
18. Bronesky D, Wu Z, Marzi S, Walter P, Geissmann T, Moreau K, et al. Staphylococcus aureus RNAIII and Its Regulon Link Quorum Sensing, Stress Responses, Metabolic Adaptation, and Regulation of Virulence Gene Expression. Annu Rev Microbiol. 2016; 70:299-316.
19. Wayne, PA.Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 24th informational supplement. CLSI M100-S24. Clinical and Laboratory Standards Institute. 2014.
20. Tristan A, Ying L, Bes M, Etienne J, Vandenesch F, Lina G. Use of Multiplex PCR To Identify Staphylococcus aureus Adhesins Involved in Human Hematogenous Infections. Journal of Clinical Microbiology. 2003; 41(9):4465-7.
21. Saderi H, Emadi B, Owlia P. Phenotypic and genotypic study of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of Staphylococcus aureus in Tehran, Iran. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research. 2011; 17(2):BR48-BR53.
22. Seyedi Marghaki F, Hosseini nave H, Kalantar-Neyestanaki D, Safaari F, Fasihi Y, Moradi M. Frequency of Aminoglycoside-Resistance Genes in Methicillin Resistant Staphylococcus Aureus Isolated from Clinical Specimens. Journal of Mazandaran University of Medical Sciences. 2017; 27(153):112-7.
23. Thompson T, Brown PD. Comparison of antibiotic resistance, virulence gene profiles, and pathogenicity of methicillin-resistant and methicillin-susceptible Staphylococcus aureus using a Caenorhabditis elegans infection model. Pathogens and Global Health. 2014; 108(6):283-91.
24. Ghasemian A, Najar Peerayeh S, Bakhshi B, Mirzaee M. The Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) Genes among Clinical Isolates of Staphylococcus aureus from Hospitalized Children. Iranian journal of pathology. 2015; 10(4):258-64.
25. Shafaei E, honarmand Jahromy s, Noorbakhsh F. Investigation of fnBP and clf genes prevalence among Methicillin-resistant Staphylococcus aureus strains and assessment of the effects of host factors and clinical specimens on their distribution. Journal of Fasa University of Medical Sciences. 2017; 7(1):94-103.
26. Anderson AS, Scully IL, Buurman ET, Eiden J, Jansen KU. Staphylococcus aureus Clumping Factor A Remains a Viable Vaccine Target for Prevention of S. aureus Infection. mBio. 2016; 7(2): 00225-16.
27. Botelho J, Grosso F, Quinteira S, Mabrouk A, Peixe L. The complete nucleotide sequence of an IncP-2 megaplasmid unveils a mosaic architecture comprising a putative novel blaVIM-2-harbouring transposon in Pseudomonas aeruginosa. J Antimicrob Chemother. 2017; 72(8):2225-9.
28. Anderson AS, Miller AA, Donald RGK, Scully IL, Nanra JS, Cooper D, et al. Development of a multicomponent Staphylococcus aureus vaccine designed to counter multiple bacterial virulence factors. Human Vaccines & Immunotherapeutics. 2012; 8(11):1585-94.
29. Michelim L, Lahude M, Araújo PR, Giovanaz DSH, Müller G, Delamare APL, et al. Pathogenic factors and antimicrobial resistance of Staphylococcus epidermidis associated with nosocomial infections occurring in intensive care units. Brazilian Journal of Microbiology. 2005; 36:17-23.
30. Iqbal Z, Seleem MN, Hussain HI, Huang L, Hao H, Yuan Z. Comparative virulence studies and transcriptome analysis of Staphylococcus aureus strains isolated from animals. Scientific reports. 2016; 6:35442.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
