Volume 22, Issue 1 (4-2019)                   J Arak Uni Med Sci 2019, 22(1): 27-38 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Parvin A, Yaghmaei P, Noureddini M, Haeri Roohani A, Aminzadeh S. Comparative Investigation of the Effect of Quercetin and Hydroalcoholic Extract of Otostegia Persica Boiss with Atorvastatin on ABC A1 Gene Expression in Hypercholesterolemic Male Wistar Rats. J Arak Uni Med Sci 2019; 22 (1) :27-38
URL: http://jams.arakmu.ac.ir/article-1-5799-en.html
1- Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Applied Cell Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran. , mnoureddini@yahoo.com
3- Department of Bioprocess Engineering Research, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Abstract:   (2560 Views)
Background and Aim: Hypercholesterolemia is an independent risk factor for atherosclerosis that the use of medicinal plants with minimal side effects is very important in the treatment of it. In this study, comparative evaluation of the effect of hydroalcoholic extract and quercetin of Otostegia persica Boiss with atorvastatin on ABC A1 gene expression in hypercholesterolemic male Wistar rats was carried out.
Materials and Methods: Forty male wistar rats with about 180gr weight randomly individed into five groups of eight: 3 experimental groups, 1 sham group and 1 control group. The experimental and sham groups received a high-fat diet with 2% cholesterol (through gavage) for 40 days. The experimental groups were treated (were fed) separately with 40 mg/kg/day atorvastatin, 25 mg/kg/day quercetin and 25 mg/kg/day hydroalcoholic extract of Otostegia persica Boiss for 28 days. Sham group received daily 1 mg/kg saline water during this period. In the end, the expression of ABC A1 gene was determined by Real-Time PCR in leukocytes and serum lipids were measured by photometric method.
Ethical Considerations: This study with research ethics code B/29/5/1/1799 has been approved by committee for ethics in biomedical research at Kashan university of medical sciensec on July 31, 2016.
Findings: The hydroalcoholic extract and quercetin of Otostegia persica Boiss and atorvastatin significantly increased ABC A1 gene expression in three experimental groups {(1.14 ± 0.09) ,(1.18 ± 0.03),(1.11 ± 0.03) respectively} realated to control group(1.00 ± 0.011) (p<0.05) and sham group(0.89 ± 0.03) (p<0.05). Quercetin also was more effective than atorvastatin in increasing of ABC A1 gene expression (p<0.05).
Conclusion: Quercetin and hydroalcoholic extract of Otostegia persica Boiss have increased effect on ABC A1 gene expression in hypercholesterolemic male Wistar rats.
Full-Text [PDF 579 kb]   (796 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2018/06/7 | Accepted: 2018/09/17

References
1. Herrington W, Lacey B, Sherliker P, Armitage J, Lewington S. Epidemiology of atherosclerosis and the potential to reduce the global burden of atherothrombotic disease. Circulation research. 2016; 118(4):535-46.
2. Shanmugam N, Roman-Rego A, Ong P, Kaski JC. Atherosclerotic plaque regression: fact or fiction? Cardiovascular drugs and therapy. 2010; 24(4):311-7.
3. Hayden MR, Clee SM, Brooks-Wilson A, Genest Jr J, Attie A, Kastelein JJ. Cholesterol efflux regulatory protein, Tangier disease and familial high-density lipoprotein deficiency. Current opinion in lipidology. 2000; 11(2):117-22.
4. Vasiliou V, Vasiliou K, Nebert DW. Human ATP-binding cassette (ABC) transporter family. Human genomics. 2009; 3(3):281-90.
5. Langmann T, Mauerer R, Zahn A, Moehle C, Probst M, Stremmel W, et al. Real-time reverse transcription-PCR expression profiling of the complete human ATP-binding cassette transporter superfamily in various tissues. Clinical chemistry. 2003; 49(2):230-8.
6. Song G, Liu J, Zhao Z, Yu Y, Tian H, Yao S, et al. Simvastatin reduces atherogenesis and promotes the expression of hepatic genes associated with reverse cholesterol transport in apoE-knockout mice fed high-fat diet. Lipids in health and disease. 2011; 10(1):8-14.
7. Shimizu T, Miura S-i, Tanigawa H, Kuwano T, Zhang B, Uehara Y, et al. Rosuvastatin Activates ATP-Binding Cassette Transporter A1–Dependent Efflux Ex Vivo and Promotes Reverse Cholesterol Transport in Macrophage Cells in Mice Fed a High-Fat DietSignificance. Arteriosclerosis, thrombosis, and vascular biology. 2014; 34(10):2246-53.
8. Genvigir FDV, Rodrigues AC, Cerda A, Arazi SS, Willrich MAV, Oliveira R, et al. Effects of lipid-lowering drugs on reverse cholesterol transport gene expressions in peripheral blood mononuclear and HepG2 cells. Pharmacogenomics. 2010; 11(9):1235-46.
9. Taylor F, Ward K, Moore T, Burke M, Davey Smith G, Casas JP, et al. Statins for the primary prevention of cardiovascular disease. Cochrane database syst rev. 2011; 1(1):1-72.
10. Shrififar F, Yassa F, Shafiee A. Antioxidant activity of Otostegia persica (Labiatae) and its constituents. Iranian Journal of Pharmaceutical Research. 2010; 2(4):235-9.
11. Vecera R, Zacharova A, Orolin J, Skottova N, Anzenbacher P. The effect of silymarin on expression of selected ABC transporters in the rat. Veterinarni Medicina 2011; 56 (2): 59–62.
12. Yeh YT, Chiang AN, Hsieh SC. Chinese Olive (Canarium album L.) Fruit Extract Attenuates Metabolic Dysfunction in Diabetic Rats. Nutrients 2017, 9, 1123; doi:10.3390/nu9101123.pages:1-18.
13. Sadeghi Z, Akaberi M, Valizadeh J. Otostegia persica (Lamiaceae): A review on its ethnopharmacology, phytochemistry, and pharmacology. Avicenna journal of phytomedicine. 2014; 4(2):79-88.
14. Amna B, Uzma S, Umme HH, Farha A, Ambreen MU. Hyperlipidemia and hypertension; cardiovascular risk factors, various induction methods and their management by ethnomedicines. International Research Journal of Pharmacy. 2016; 7(11):1-9.
15. Sharifi N, Mahernia S, Amanlou M. Comparison of Different Methods in Quercetin Extraction from Leaves of Raphanus sativus L. Pharmaceutical Sciences. 2017; 23(1):59-65.
16. Lekar A, Borisenko S, Vetrova E, Sushkova S, Borisenko N. Extraction of quercetin from Polygonum hydropiper L. by subcritical water. American Journal of Agricultural and Biological Sciences. 2014; 9(1):1-5.
17. Boyd M, Tennant S, Melendez J, Toema D, Galen J, Geddes C, et al. Adaptation of red blood cell lysis represents a fundamental breakthrough that improves the sensitivity of Salmonella detection in blood. Journal of applied microbiology. 2015; 118(5):1199-209.
18. Hamidinia M, Boroujerdnia MG, Talaiezadeh A, Solgi G, Roshani R, Iranprast S, et al. Increased P-35, EBI3 transcripts and other treg markers in peripheral blood mononuclear cells of breast cancer patients with different clinical Stages. Advanced pharmaceutical bulletin. 2015; 5(2):261-7.
19. Chen Z, Lei Y-L, Wang W-P, Lei Y-Y, Liu Y-H, Hei J, et al. Effects of Saponin from Trigonella Foenum-Graecum Seeds on Dyslipidemia. Iranian journal of medical sciences. 2017; 42(6):577-85.
20. Liu H-Y, Cui H-B, Chen X-M, Chen X-Y, Wang S-H, Du W-P, et al. Imbalanced response of ATP-binding cassette transporter A1 and CD36 expression to increased oxidized low-density lipoprotein loading contributes to the development of THP-1 derived foam cells. The Journal of Biochemistry. 2014; 155(1):35-42.
21. Wang M-D, Franklin V, Marcel YL. In vivo reverse cholesterol transport from macrophages lacking ABCA1 expression is impaired. Arteriosclerosis, thrombosis, and vascular biology. 2007; 27(8):1837-42.
22. Liao H, Langmann T, Schmitz G, Zhu Y. Native LDL upregulation of ATP-binding cassette transporter-1 in human vascular endothelial cells. Arteriosclerosis, thrombosis, and vascular biology. 2002; 22(1):127-32.
23. Su X-M, Wei Y, Wang Y, Zhang W. ABCA1 mRNA expression and cholesterol outflow in U937 cells. International journal of clinical and experimental pathology. 2015; 8(3):3116-21.
24. Ahmadi Y, Ghorbanihaghjo A, Argani H. The effect of statins on the organs: similar or contradictory? Journal of cardiovascular and thoracic research. 2017; 9(2):64-70.
25. Srinivas M, Annapurna A, Reddy YN. Anti-atherosclerotic effect of atorvastatin and clopidogrel alone and in combination in rats. Indian J Exp Biol. 2008; 46(10):698-703.
26. Argmann CA, Edwards JY, Sawyez CG, O'Neil CH, Hegele RA, Pickering JG, et al. Regulation of macrophage cholesterol efflux through hydroxymethylglutaryl-CoA reductase inhibition a role for RhoA in ABCA1-mediated Cholesterol efflux. Journal of Biological Chemistry. 2005; 280(23):22212-21.
27. Lakhanpal P, Rai DK. Role of quercetin in cardiovascular diseases. Internet Journal of Medical Update. 2008; 3(1):31-49.
28. Chis IC, Coseriu A, Simedrea R, Oros A, Nagy AL, Clichici S. In Vivo Effects of Quercetin in Association with Moderate Exercise Training in Improving Streptozotocin-Induced Aortic Tissue Injuries. Molecules. 2015; 20(12):21770-86.
29. Dourandishan M, Hossieni M, Malekaneh M, Bagherzade G. Effect of Otostegia persica’s root extract on the blood biochemical factors in diabetic hyperlipidemic rats. The Horizon of Medical Sciences. 2014; 20(1):17-21.
30. Bagherzade G, Dourandishana M, Malekanehb M. Antidiabetic effects of Otostegia persica root in alloxan-induced diabetic rats. Pure Appl Chem Sci. 2014; 2:1-9.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb