Volume 22, Issue 2 (6-2019)
J Arak Uni Med Sci 2019, 22(2): 96-105 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Moeini A, Farsi S, Moghaddasi M. The Effect of Curcumin Supplementation on Expression of Regulatory Signaling Genes of Cardiac Muscle Growth Messenger in Obese Male Rats. J Arak Uni Med Sci 2019; 22 (2) :96-105
URL: http://jams.arakmu.ac.ir/article-1-5941-en.html
URL: http://jams.arakmu.ac.ir/article-1-5941-en.html
1- Department of Exercises Physiology, Larestan Branch, Islamic Azad University, Fars, Iran.
2- Department of Exercises Physiology, Larestan Branch, Islamic Azad University, Fars, Iran. ,sirous.farsi@gmail.com
3- Department of Exercises Physiology, Shiraz Branch, Islamic Azad University, Fars, Iran.
2- Department of Exercises Physiology, Larestan Branch, Islamic Azad University, Fars, Iran. ,
3- Department of Exercises Physiology, Shiraz Branch, Islamic Azad University, Fars, Iran.
Abstract: (2556 Views)
Background and Aim: Curcumin is one of the most important nutritional polyphenols that is included in daily supplements diet highly and plays a role in moderating some of the intracellular messenger pathways associated with the regulation of pathologic hypertrophy. The purpose of the present study is to survey the effect of curcumin supplementation on the expression of some genes regulators of the pathological processes of the heart muscle in rats.
Materials and Methods: In this eight-week experimental study, 12 male Sprague Dawley rats were divided into two groups: obesity control (n=6) and curcumin supplement (n=6). Curcumin supplementation was conducted for eight weeks. 24 hours after the completion of the curcumin supplement protocol, the rats were dissected and their heart muscle was removed. The expression of the genes (AMPK, mTOR, S6K, 4EBP, COL1, COL3, and Ang) was performed using Real-Time PCR technique. The expression of the genes was calculated by the 2-∆∆CT method. One way ANOVA was applied to determine the significance of the variables among the study groups.
Ethical Considerations: This study with research ethics code IR.SUMS.REC.1396.S446 has been approved by research ethics committee at Shiraz University of Medical Sciences.
Findings:The results has showed that supplement group of curcumin reduced the expression of mTOR (p < 0.001), S6K (p < 0.011), 4EBP (p > 0.005) collagen 1 (p > 0.002), Collagen 3 (p < 0.001) and Ang (p < 0.003) compared to the placebo group. There was also an increase in the expression of AMPK gene (p < 0.001) which was statistically significant.
Conclusion: it seems that the supplementation of 10 mg curcumin moderate the pathological pathway of cardiac muscle hypertrophy by reducing or keeping up the expression of mTOR gene in obese rats and increasing the expression of AMPK gene. Moreover, this supplement can affect on reducing the pathological hypertrophy during the consumption of curcumin supplementation
Materials and Methods: In this eight-week experimental study, 12 male Sprague Dawley rats were divided into two groups: obesity control (n=6) and curcumin supplement (n=6). Curcumin supplementation was conducted for eight weeks. 24 hours after the completion of the curcumin supplement protocol, the rats were dissected and their heart muscle was removed. The expression of the genes (AMPK, mTOR, S6K, 4EBP, COL1, COL3, and Ang) was performed using Real-Time PCR technique. The expression of the genes was calculated by the 2-∆∆CT method. One way ANOVA was applied to determine the significance of the variables among the study groups.
Ethical Considerations: This study with research ethics code IR.SUMS.REC.1396.S446 has been approved by research ethics committee at Shiraz University of Medical Sciences.
Findings:The results has showed that supplement group of curcumin reduced the expression of mTOR (p < 0.001), S6K (p < 0.011), 4EBP (p > 0.005) collagen 1 (p > 0.002), Collagen 3 (p < 0.001) and Ang (p < 0.003) compared to the placebo group. There was also an increase in the expression of AMPK gene (p < 0.001) which was statistically significant.
Conclusion: it seems that the supplementation of 10 mg curcumin moderate the pathological pathway of cardiac muscle hypertrophy by reducing or keeping up the expression of mTOR gene in obese rats and increasing the expression of AMPK gene. Moreover, this supplement can affect on reducing the pathological hypertrophy during the consumption of curcumin supplementation
References
1. Lima AR, Martinez PF, Okoshi K, Guizoni DM, Zornoff LA, Campos DH, Oliveira Jr SA, Bonomo C, Dal Pai‐Silva M, Okoshi MP. Myostatin and follistatin expression in skeletal muscles of rats with chronic heart failure. International journal of experimental pathology. 2010; 91(1):54-62.
2. Kaplan LM, Golden A, Jinnett K, Kolotkin RL, Kyle TK, Look M, Nadglowski J, O'Neil PM, Parry T, Tomaszewski KJ, Stevenin B. Perceptions of barriers to effective obesity care: results from the national ACTION study. Obesity. 2018; 26(1):61-9.
3. Sutaria S, Devakumar D, Yasuda SS, Das S, Saxena S. Is obesity associated with depression in children? Systematic review and meta-analysis. Archives of disease in childhood. 2019; 104(1):64-74.
4. Adabag S, Huxley RR, Lopez FL, Chen LY, Sotoodehnia N, Siscovick D, Deo R, Konety S, Alonso A, Folsom AR. Obesity related risk of sudden cardiac death in the atherosclerosis risk in communities study. Heart. 2015; 101(3):215-21.
5. Schirone L, Forte M, Palmerio S, Yee D, Nocella C, Angelini F, Pagano F, Schiavon S, Bordin A, Carrizzo A, Vecchione C. A review of the molecular mechanisms underlying the development and progression of cardiac remodeling. Oxidative medicine and cellular longevity. 2017; 2017.
6. Bujak M, Frangogiannis NG. The role of TGF-β signaling in myocardial infarction and cardiac remodeling. Cardiovascular research. 2007; 74(2):184-95.
7. Fang CX, Dong F, Thomas DP, Ma H, He L, Ren J. Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription. American Journal of Physiology-Heart and Circulatory Physiology. 2008; 295(3):H1206-15.
8. Khan SA, Salloum F, Das A, Xi L, Vetrovec GW, Kukreja RC. Rapamycin confers preconditioning-like protection against ischemia–reperfusion injury in isolated mouse heart and cardiomyocytes. Journal of molecular and cellular cardiology. 2006; 41(2): 256-64.
9. Das A, Durrant D, Koka S, Salloum FN, Xi L, Kukreja RC. Mammalian target of rapamycin (mTOR) inhibition with rapamycin improves cardiac function in type 2 diabetic mice potential role of attenuated oxidative stress and altered contractile protein expression. Journal of Biological Chemistry. 2014; 289(7):4145-60.
10. Hardie DG. AMPK: positive and negative regulation, and its role in whole-body energy homeostasis. Current opinion in cell biology. 2015; 33:1-7.
11. Faubert B, Vincent EE, Poffenberger MC, Jones RG. The AMP-activated protein kinase (AMPK) and cancer: many faces of a metabolic regulator. Cancer letters. 2015; 356(2):165-70.
12. Zang M, Xu S, Maitland-Toolan KA, Zuccollo A, Hou X, Jiang B, Wierzbicki M, Verbeuren TJ, Cohen RA. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor–deficient mice. Diabetes. 2006; 55(8):2180-91.
13. Zhai X, Qiao H, Guan W, Li Z, Cheng Y, Jia X, Zhou Y. Curcumin regulates peroxisome proliferator-activated receptor-γ coactivator-1α expression by AMPK pathway in hepatic stellate cells in vitro. European journal of pharmacology. 2015; 746:56-62.
14. Tong W, Wang Q, Sun D, Suo J. Curcumin suppresses colon cancer cell invasion via AMPK-induced inhibition of NF-κB, uPA activator and MMP9. Oncology letters. 2016; 12(5):4139-46.
15. Xiao J, Sheng X, Zhang X, Guo M, Ji X. Curcumin protects against myocardial infarction-induced cardiac fibrosis via SIRT1 activation in vivo and in vitro. Drug design, development and therapy. 2016; 10:1267.
16. Salesi M, Mehrtash M, Daryanoosh F, Tanide N. The Role of Caloric Restriction on Lipid Coat Proteins Gene Expression and Insulin Resistance after 8 Weeks High Caloric Diet in Male Rats. Journal of Arak University of Medical Sciences. 2018; 21(5):21-31.
17. Na LX, Zhang YL, Li Y, Liu LY, Li R, Kong T, Sun CH. Curcumin improves insulin resistance in skeletal muscle of rats. Nutrition, Metabolism and Cardiovascular Diseases. 2011; 21(7):526-33.
18. Li HL, Liu C, De Couto G, Ouzounian M, Sun M, Wang AB, Huang Y, He CW, Shi Y, Chen X, Nghiem MP. Curcumin prevents and reverses murine cardiac hypertrophy. The Journal of clinical investigation. 2008; 118(3):879-93.
19. Marcu MG, Jung YJ, Lee S, Chung EJ, Lee MJ, Trepel J, Neckers L. Curcumin is an inhibitor of p300 histone acetylatransferase. Medicinal chemistry. 2006; 2(2):169-74.
20. Morimoto T, Sunagawa Y, Kawamura T, Takaya T, Wada H, Nagasawa A, Komeda M, Fujita M, Shimatsu A, Kita T, Hasegawa K. The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats. The Journal of clinical investigation. 2008; 118(3):868-78.
21. Epstein J, Sanderson IR, MacDonald TT. Curcumin as a therapeutic agent: the evidence from in vitro, animal and human studies. British journal of nutrition. 2010; 103(11): 1545-57.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
