Volume 22, Issue 2 (6-2019)
J Arak Uni Med Sci 2019, 22(2): 11-21 |
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Pishgar E, Makhfian M. The Evaluation of Five Plant Extracts Inhibitory Potential against Bacterial Quorum Sensing of Staphylococcus Aureus. J Arak Uni Med Sci 2019; 22 (2) :11-21
URL: http://jams.arakmu.ac.ir/article-1-5956-en.html
URL: http://jams.arakmu.ac.ir/article-1-5956-en.html
1- Department of Microbiology, Islamic Azad University of Jahrom, Fars, Iran. , elhampishgar1504@gmail.com
2- Department of Plant Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Plant Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Abstract: (2967 Views)
Background and Aim: A variety of bacteria like Staphylococcus aureus utilize quorum sensing to perform their important activities such as biofilm formation and production of virulence factors. Interfering with the bacterial QS will disable the bacteria to perform above-mentioned vital activities. The principal aim of the study was to evaluate the effects of five plant extracts against bacterial Quorum sensing of Staphylococcus aureus.
Materials and Methods: Thirteen strains of Staphylococcus aureus were isolated from patients with dental implant infection and identified. The plant species were collected from vicinity gardens of Fars Province and extracted using 96% ethanol. The anti-QS and antimicrobial susceptibility methods were then carried out to evaluate their bactericidal and QS properties with the use of Agrobacterium tumefaciens NTL/PZLR4. Furthermore, the biofilm production of the isolates was evaluated by microtiter plate (MTP) assay.
Ethical Considerations: This study with research ethics codes 01-16-1-1959 and 73/118248 has been approved by research ethics committee at Islamic Azad University, Jahrom and research sciences branch, respectively.
Findings: The results of the study disclosed that the extract of raspberry blossom possesses significantly (p<0.05) anti-QS property (>21 mm). The anti-QS activity was proved by creating clear halo sides of the wells formed by Agrobacterium tumefaciens NTL/PZLR4. Moreover, the extracts of tarragon, wheat flower, flixweed and basil showed antimicrobial properties.
Conclusion: According to the anti-biofilm and anti-QS properties of raspberry blossom extract against the isolates of Staphylococcus aureus, it could be considered as a mouthwash against dental bacterial infection with the identification of active compounds in the raspberry.
Materials and Methods: Thirteen strains of Staphylococcus aureus were isolated from patients with dental implant infection and identified. The plant species were collected from vicinity gardens of Fars Province and extracted using 96% ethanol. The anti-QS and antimicrobial susceptibility methods were then carried out to evaluate their bactericidal and QS properties with the use of Agrobacterium tumefaciens NTL/PZLR4. Furthermore, the biofilm production of the isolates was evaluated by microtiter plate (MTP) assay.
Ethical Considerations: This study with research ethics codes 01-16-1-1959 and 73/118248 has been approved by research ethics committee at Islamic Azad University, Jahrom and research sciences branch, respectively.
Findings: The results of the study disclosed that the extract of raspberry blossom possesses significantly (p<0.05) anti-QS property (>21 mm). The anti-QS activity was proved by creating clear halo sides of the wells formed by Agrobacterium tumefaciens NTL/PZLR4. Moreover, the extracts of tarragon, wheat flower, flixweed and basil showed antimicrobial properties.
Conclusion: According to the anti-biofilm and anti-QS properties of raspberry blossom extract against the isolates of Staphylococcus aureus, it could be considered as a mouthwash against dental bacterial infection with the identification of active compounds in the raspberry.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2018/11/22 | Accepted: 2019/04/22
Received: 2018/11/22 | Accepted: 2019/04/22
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