Volume 22, Issue 5 (11-2019)                   J Arak Uni Med Sci 2019, 22(5): 32-43 | Back to browse issues page


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Mondanizadeh M, Moradi N, Amini R, Khansarinejad B, Mosayebi G. Bioinformatic Prediction of miRNAs Targeting APRIL and BAFF Genes in Chronic Lymphocytic Leukemia. J Arak Uni Med Sci 2019; 22 (5) :32-43
URL: http://jams.arakmu.ac.ir/article-1-6105-en.html
1- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak University of Medical Sciences, Arak, Iran.; Department of Biotechnology and Molecular Medicine, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
2- Department of Biotechnology and Molecular Medicine, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
3- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak University of Medical Sciences, Arak, Iran.; Department of Microbiology and Immunology, School of Paramedicine, Arak University of Medical Sciences, Arak, Iran.
4- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak University of Medical Sciences, Arak, Iran.; Department of Microbiology and Immunology, School of Paramedicine, Arak University of Medical Sciences, Arak, Iran. , gmosayebi@yahoo.com
Abstract:   (2165 Views)
Background and Aim Chronic Lymphocytic Leukemia (CLL) is the most commonly occurring leukemia in adults, accounting for about 30-25% of total leukemia. One of the important etiological causes of this leukemia is the disruption of the Nuclear Factor Kappa B (NF-kB) signaling pathway. The two proteins of Apoptosis-Inducing Ligand (APRIL) and B-Cell Activating Factor (BAFF) play a role in the pathogenesis of this leukemia by affecting the NF-kB signaling pathway. In this study, due to the effect of miRNAs in regulating many cellular processes, the prediction of the prominent miRNAs targeting APRIL and BAFF transcripts in B-cell CLL patients was evaluated using specific and different bioinformatics programs.
Methods & Materials Afterwards retrieving the sequences of APRIL and BAFF proteins from the NCBI website, by using several programs including miRanda, TargetScan, miRWalk, DIANA and miRDB with different algorithms, the prediction of miRNAs targeting these genes was investigated.
Ethical Considerations This study was approved by the Research Ethics Committee of Arak University of Medical Sciences.
Results Based on the scoring system of bioinformatics programs, “hsa-miR-145-5p” and “hsa-miR-185-5p” were identified as miRNAs targeting APRIL gene, while “hsa-miR-424” and “hsa-miR-497”were miRNAs targeting BAFF gene. They were suggested for the practical studies in future.
Conclusion Based on the important role of APRIL and BAFF genes in the normal process of cell death and B-cell evolution, it seems that the mi-RNAs predicted by bioinformatics programs using different algorithms can be used as a diagnostic molecular biomarker to identify B-cell CLL patients.
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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2019/07/8 | Accepted: 2019/08/27

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