Volume 23, Issue 6 (February & March 2020)                   J Arak Uni Med Sci 2020, 23(6): 902-911 | Back to browse issues page

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Morad A, Zeinoddini M. Enrichment and Rapid Detection of Vibrio Cholerae From Water by Non-culture Method. J Arak Uni Med Sci 2020; 23 (6) :902-911
URL: http://jams.arakmu.ac.ir/article-1-6212-en.html
1- Department of Biology, Faculty of Chemical Engineering, Malek-e-Ashtar University of Technology, Shahin Shahr, Iran.
2- Department of Biology, Faculty of Chemical Engineering, Malek-e-Ashtar University of Technology, Shahin Shahr, Iran. , zeinoddini52@mut.ac.ir
Abstract:   (1958 Views)
Background and Aim: In the microbial contamination of food and water, identifying the trace amounts of contaminating bacteria has always been of researchers’ interest and concern. The most frequent approach to resolve this problem is using culture-based methods to increase and enrich bacteria samples; accordingly, it extends the bacterial detection process to several hours or days. One of the smart strategies to solve this problem is the concentration of bacteria using physical methods. The present study aimed to enrich Vibrio cholerae as the most essential water-polluting germs. Accordingly, we used the filtration method and evaluated its function by culture method and two detection approaches of Adenosine Triphosphate (ATP) and PCR assay.
Methods & Materials: A certain concentration of V. Cholerae was artificially added to a specified volume of sterile water. Then, the bacteria were extracted from the medium and filtered using 0.450 µm separable filters. Finally, the performance of the pre- and post-filtration processes was compared using bacterial cell culture (CFU), ATP, and PCR assay with the specific primers for the ompW gene of V. cholerae.
Ethical Considerations: This article is a meta-analysis with no human or animal sample.
Results: The present research results indicated that the applied method presented high efficiency and recovery performance. In other words, samples provided no positive response before filtration in both methods; however, after filtration in isolated and recovered samples, the presence of bacteria was detected in the ATP and PCR methods. 
Conclusion: In conclusion, the employed strategy can detect V. cholerae in non-culture and in the shortest time in contaminated water samples.
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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2019/12/29 | Accepted: 2020/08/11

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