Volume 24, Issue 2 (June & July 2021)                   J Arak Uni Med Sci 2021, 24(2): 216-229 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dehghani S, Rouhi L, Ziya Jahromi N, Dehghani R, Khashei Varnamkhasti K. The Antioxidant Effects of Ginger Extract on Bioavailability and Oxidative Stress-induced Apoptosis in Mesenchymal Stem Cells of Human Adipose Tissue and Rat Bone Marrow. J Arak Uni Med Sci 2021; 24 (2) :216-229
URL: http://jams.arakmu.ac.ir/article-1-6474-en.html
1- Department of Biochemistry, Shahrekord Branch, University of Islamic Azad, Shahrekord, Iran.
2- Department of Physiology, Shahrekord Branch, University of Islamic Azad, Shahrekord, Iran. , irouhi59@gmail.com
Abstract:   (1731 Views)
Background and Aim: Proliferate potential differentiate into different cell lineages and high self-renewal of Mesenchymal Stem Cells (MSCs); thus, they are ideal tools for regenerative medicine. However, a leading problem is an oxidative stress in the target tissue and the apoptosis of transplanted stem cells before tissue repair. The pretreatment of stem cells with antioxidants may make them resistant to oxidative stress. Ginger is the main medicinal plant with antioxidant properties. This study explored the antioxidant effects of ginger extract on bioavailability and oxidative stress-induced apoptosis in human adipose tissue-derived mesenchymal stem cells and rat bone marrow examined. 
Methods & Materials: In this study, human adipose tissue-derived mesenchymal stem cells and rat bone marrow were cultured in a DMEM medium with 20% FBS. The explored cells were incubated for 4 and 6 hours for pretreatment with different concentrations of ginger extract (50, 100, 200, & 400 mg/mL); then, they were treated with 200 μM H2O2 for 2 hours. Bioavailability was analyzed by ELISA reader using an MTS kit and apoptosis was analyzed by flow cytometry using an Annexin V-FITC/PI kit into the manufacturer’s protocol at both times. The obtained data were analyzed by Analysis of Variance (ANOVA) using SPSS. 
Ethical Considerations: This study was approved by the Ethics Research Committee of Shahrekord Branch, Islamic Azad University (Code: IR.IAU.SHK.REC.1397.028).
Results: The MTS results indicated a dose- and time-dependent manner increase in the bioavailability of human adipose tissue-derived mesenchymal treated stem cells. Ginger extract treatment also dose- and time-dependently decreased the rate of apoptosis in rat bone marrow mesenchymal stem cells. 
Conclusion: Ginger extract, by reducing the oxidative stress in mesenchymal stem cells, elevates their lifespan in the target tissue, and increases the efficiency of these cells in tissue regeneration.
Full-Text [PDF 4798 kb]   (1056 Downloads) |   |   Full-Text (HTML)  (1269 Views)  
Type of Study: Original Atricle | Subject: General
Received: 2020/10/31 | Accepted: 2021/01/9

1. Le Blanc K, Rasmusson I, Sundberg B, Götherström C, Hassan M, Uzunel M, et al. Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells. Lancet 2004; 363(9419): 1439-41. [DOI:10.1016/S0140-6736(04)16104-7]
2. Kuo TK, Hung SP, Chuang CH, Chen CT, Shih YRV, Fang SCY, et al. Stem cell therapy for liver disease: parameters governing the success of using bone marrow mesenchymal stem cells. Gastroenterology 2008; 134(7): 2111-2121. [DOI:10.1053/j.gastro.2008.03.015]
3. Amit N Patel, Jorge Genovese. Potential clinical application of adult human mesenchymal stem cell (Prochymal) therapy.Stem Cell Cloning: Advances and Applications 2011:4, 61-72. [DOI:10.2147/SCCAA.S11991]
4. Nassiri Asl M, Aali E.Review on the mesenchymal stem cells and their potential application in regenerative medicine. J Qazvin Univ Med Sci 2018; 21(6): 74-89.‏ [Persian]‏ [DOI:10.29252/qums.21.6.89]
5. Pournasr Khakbaz B, Baharvand H. Human mesenchymal stem cells and their clinical application. Journal of Iranian Anatomical Sciences 2007; 5(19): 157-206. [Persian]‏
6. Parson A. The proteus effect: stem cells and their promise for medicine: Joseph Henry Press; 2004. doi: 10.1172/JCI25763. [DOI:10.1172/JCI25763]
7. Song L, Tuan RS. Transdifferentiation potential of human mesenchymal stem cells derived from bone marrow. FASEB journal: official publication of the Downloaded from Federation of American Societies for Experimental Biology. 2004; 18(9): 980-982. [DOI:10.1096/fj.03-1100fje]
8. Oubari F, Amirizade N, Mohammadpour H. The important role of FLT3-L in ex vivo expansion of hematopoietic stem cells following co-culture with mesenchymal stem cells. Cell Journal (Yakhteh) 2015; 17(2): 201.‏ [Persian]‏
9. Oubari F, Nikougoftar Zarif M, Amirizadeh N,Shaiegan M,Atarodi A,Nakhlestani et al.Isolation and expansion of Mesenchymal Stem cells from placenta. Sci J Iran Blood Transfus Organ 2013; 10: 222-230. [Persian]‏
10. Dehghani Fard A, Saki N, Ahmadvand M. Mesenchymal stem cell biology, application and its role in regenerative medicine. Scientific Journal of Iran Blood Transfus Organ 2012; 8(4): 306-320.‏ [Persian]‏
11. Ryan JM, Barry FP, Murphy JM, Mahon BP. Mesenchymal stem cells avoid allogeneic ejection. J Inflamm (Lond) 2005; 2(8): 1-11. [DOI:10.1186/1476-9255-2-8]
12. Nasiri F, Amiri F, Mohammadipour M, Molaei S. H 2 O 2-preconditioned mesenchymal stem cell regenerative effects on acute liver failure mice. Scientific Journal of Iranian Blood Transfusion Organization 2015; 12(2): 111-124. [Persian]‏
13. Baksh D, Song L, Tuan RS. Adult mesenchymal stem cells:characterization, differentiation and application in cell and gene therapy. J Cell Mol Med 2004; 8(3): 301-16. [DOI:10.1111/j.1582-4934.2004.tb00320.x]
14. Chapel A, Bertho JM Bensidhoum M, Fouillard L, Young RG, Frick J. Mesenchymal stem cells home to injure tissues when coinfused with hematopoietics cell to treat at radiation, inducedmulti, organfailure syndrome. J Gene Med 2003; 5(12): 1028-38. [DOI:10.1002/jgm.452]
15. Marquez-Curtis LA, Janowska-Wieczork A, McGann LE, Elliott JA. Mesenchymal stromal cells derived from various tissues: Biological, clinical and cryopreservation aspects. Cryobiology 2015; 71: 181-97. [DOI:10.1016/j.cryobiol.2015.07.003]
16. Nasir GA, Mohsin S, Khan M, Shams S, Ali G, Khan SN. Mesenchymal stem cells and Interleukin-6 attenuate liver fibrosis in mice. J Transl Med 2013; 11(3): 78-97. [Persian]‏ [DOI:10.1186/1479-5876-11-78]
17. Bahmani M, Saki K, Shahsavari S . Identification of medicinal plants effective in infectious diseases in Urmia, northwest of Iran. Asi Paci J Trop Biomed 2015; 5: 858-64. [Persian]‏ [DOI:10.1016/j.apjtb.2015.06.004]
18. Dadfar F, Hosseini S. E, Bahaoddini A. A review of phytochemical, pharmacological and physiological properties of ginger (zingiber officinale). Clinical Excellence 2014; 3(1): 72-86.‏ [Persian]‏
19. Haksar A, Sharma A, Chawla R, Kumar R, Arora R, Singh S, Prasad J, Gupta M, Tripathi RP, Arora MP, Islam F, Sharma RK. Zingiber officinale exhibits behavioral radioprotection against radiation. Pharmacology Biochemistry and Behavior 2006; 84: 179-188. [DOI:10.1016/j.pbb.2006.04.008]
20. Stoilova I, Krastanov A, Stoyanova A, Denev P, Gargova S. Antioxidant activity of a ginger extract (Zingiber officinale). Food Chemistry 2007; 102(3): 764-770. [DOI:10.1016/j.foodchem.2006.06.023]
21. Mirazi N, Karami Z. The protective effect of hydroalcoholic extract from rhizome of Zingiber officinale L. on carbon tetrachloride-induced hepatic injury in male rat. KAUMS Journal (FEYZ) 2016; 20(4): 297-305.‏
22. Johari H, Sharifi E, Delirnasab F, Hemayatkhah V, Kargar H, Nikpoor M. The effect of hydro-alcoholic extracts of ginger on lead detoxification of kidney in the immature wistar rats. J Rafsanjan Uni Med Sci 2013; 12(6): 417-24. [Persian]
23. Khoshtabiat L, Mahdavi M. The role of oxidative stress in proliferation and cell death. Journal of Mazandaran University of Medical Sciences 2015; 25(127): 130-145.‏ [Persian]‏
24. Halliwell B. Free radicals and antioxidants - quo vadis? Trends Pharmacol Sci 2011; 3(32): 30- 125. [DOI:10.1016/j.tips.2010.12.002]
25. Aeschbach R, Löliger J, Scott B. C, Murcia A. Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food and chemical toxicology 1994; 32(1): 31-36.‏ [DOI:10.1016/0278-6915(84)90033-4]
26. Kim JK, Kim Y. Kim TY. Gingerol prevents UVB induced Ros production and cox-2 expression invitro and invivo. Free Rad Res 2007; 41:603-14. [DOI:10.1080/10715760701209896]
27. Dugasani S, Pichika M. R. Comparative antioxidant and anti-inflammatory effects of [6]-gingerol,[8]-gingerol,[10]-gingerol and [6]-shogaol. Journal of ethnopharmacology 2010; 127(2): 515-520.‏ [DOI:10.1016/j.jep.2009.10.004]
28. Ajayi B. O, Adedara I. A. Pharmacological activity of 6‐gingerol in dextran sulphate sodium‐induced ulcerative colitis in BALB/c mice. Phytotherapy Research 2015; 29(4): 566-572.‏ [DOI:10.1002/ptr.5286]
29. Abolaji A. O, Ojo M, Afolabi T. T, Arowoogun M. D. Protective properties of 6-gingerol-rich fraction from Zingiber officinale (Ginger) on chlorpyrifos-induced oxidative damage and inflammation in the brain, ovary and uterus of rats. Chemico-Biological Interactions 2017; 270: 15-23.‏ [DOI:10.1016/j.cbi.2017.03.017]
30. Ajayi B. O, Adedara I. A. 6-Gingerol abates benzo [a] pyrene-induced colonic injury via suppression of oxido-inflammatory stress responses in BALB/c mice. Chemico-biological interactions 2019; 307: 1-7.‏ [DOI:10.1016/j.cbi.2019.04.026]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb