Volume 26, Issue 1 (April & May 2023)                   J Arak Uni Med Sci 2023, 26(1): 11-17 | Back to browse issues page


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Shifteh S Z, Hamta A. Study of some Polymorphisms of CTLA-4 Gene in Women with Breast Cancer–Markazi Province. J Arak Uni Med Sci 2023; 26 (1) :11-17
URL: http://jams.arakmu.ac.ir/article-1-7193-en.html
1- MSc Student in Genetics, Department of Biology, Faculty of Basic Sciences, University of Arak, Arak, Iran , z.shifteh96@gmail.com
2- Associate Professor, Department of Biology, Faculty of Sciences, University of Arak, Arak, Iran
Abstract:   (178 Views)
Introduction: Breast cancer is a highly heterogeneous disease. The antigen molecule of four cytotoxic T-lymphocytes is involved in inhibition of T cell response and immune response regulation. Single nucleotide polymorphisms in the CTLA4 gene can affect the expression of the aforementioned molecule. The aim of this study was to investigate the polymorphisms of rs4553808 and rs733618 of CTLA4 gene with the risk of breast cancer.
Methods: In this study to investigation polymorphisms, the DNA of 80 patients with breast cancer and 80 healthy individuals in central province of ARAK were extracted from peripheral blood. Then, PCR-RFLP technique was used. The results were analyzed using SPSS software and SNP Analyzer. This study was approved by the Ethics Committee of the Arak University (Code: Ir.arakmu.rec.1396.25).
Results: Statistical analysis rs4553808 polymorphism showed no significant increase in the risk of patients with GG genotype compared with the control group (OR = 2/013, CI = 95% 1/721-2/353). Also, heterozygotes AG genotype analysis did not show any relationship between the genetic diversity and breast cancer (OR = 1/204, CI = 95% 0/604-2/402). The combination of AG + GG genotypes did not show any significant correlations (OR = 1/130, CI = 95% 0/569-2/242). Statistical analysis for rs733618 polymorphism showed increase in the risk of breast cancer. The results indicate that the TC (OR = 2/992, CI = 95% 1/280-1/998) showed a significant relationship between the genetic diversity and breast cancer. The analysis of the combined CC and TC genotypes was associated with increased risk for breast cancer compared to TT genotypes (OR = 0/334, CI = 95%; 0.143-0.782, P = 0.009). Considering that the distribution of CC and TC genotypes was significant between the two groups of control and the patient, so the frequency of TT genotype with the same amount of P = 0.001 was significant between the two groups of control and the patient.
Conclusions: There was a significant relationship between the genotypes rs733618 polymorphism and breast cancer. However, there was no significant relationship between rs4553808 polymorphism and breast cancer risk.
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Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2022/05/27 | Accepted: 2023/09/3

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