Volume 26, Issue 4 (October & November 2023)
J Arak Uni Med Sci 2023, 26(4): 8-13 |
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Abbaspour Naderi E, Bepouei M A, Diar M, mohamadi M, Hedayati M, Shahriarinour M. Investigation of qnrB and qnrS genes frequency in E.Coli and Klebsiella Pneumoniae isolates in children with UTI in Rasht city. J Arak Uni Med Sci 2023; 26 (4) :8-13
URL: http://jams.arakmu.ac.ir/article-1-7513-en.html
URL: http://jams.arakmu.ac.ir/article-1-7513-en.html
Ensiyeh Abbaspour Naderi1 
, Mohammad Ali Bepouei1 , Mahzad Diar1 , Matin Mohamadi1 , Mohammad Hedayati2 , Mahdi Shahriarinour 1
, Mohammad Ali Bepouei1 , Mahzad Diar1 , Matin Mohamadi1 , Mohammad Hedayati2 , Mahdi Shahriarinour 1
1- Department of Biology, Faculty of Sciences, Rasht branch, Islamic Azad University, Rasht, Iran.
2- Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran
2- Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran
Abstract: (622 Views)
Introduction: Urinary tract infection (UTI) is one of the most important and common infections in children. The aim of this study was to investigate the frequency of qnrB and qnrS genes in Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections of children in 17 Shahrivar Hospital in Rasht.
Materials and methods: In this descriptive cross-sectional study, 49 strains of Escherichia coli and Klebsiella pneumoniae were isolated from 17 Shahrivar Hospital in Rasht and identified using biochemical methods. Sensitivity and resistance of strains to antibiotics were determined by Kirby Bohr and dilution broth methods. PCR method was used to evaluate the frequency of qnrS and qnrB genes in isolates.
Results: In this study, the highest resistance was observed in piperacillin (81.5%) and cefazolin (88.9%) isolates from Escherichia coli and in Klebsiella pneumoniae (cefazolin (90.9%) and amoxicillin (95.5%) isolates from 49 Isolated, 73.4% had qnrB gene and 97.9% had qnrS gene.
Conclusion: It seems that one of the reasons for increasing multidrug resistance in hospital isolates of urinary tract infection (UTI) in Rasht is the increased transfer of plasmid genes between these isolates.
Materials and methods: In this descriptive cross-sectional study, 49 strains of Escherichia coli and Klebsiella pneumoniae were isolated from 17 Shahrivar Hospital in Rasht and identified using biochemical methods. Sensitivity and resistance of strains to antibiotics were determined by Kirby Bohr and dilution broth methods. PCR method was used to evaluate the frequency of qnrS and qnrB genes in isolates.
Results: In this study, the highest resistance was observed in piperacillin (81.5%) and cefazolin (88.9%) isolates from Escherichia coli and in Klebsiella pneumoniae (cefazolin (90.9%) and amoxicillin (95.5%) isolates from 49 Isolated, 73.4% had qnrB gene and 97.9% had qnrS gene.
Conclusion: It seems that one of the reasons for increasing multidrug resistance in hospital isolates of urinary tract infection (UTI) in Rasht is the increased transfer of plasmid genes between these isolates.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2023/09/27 | Accepted: 2024/05/9
Received: 2023/09/27 | Accepted: 2024/05/9
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