Volume 27, Issue 5 (12-2024)                   J Arak Uni Med Sci 2024, 27(5): 271-278 | Back to browse issues page


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Khaki A, Baazm M, Bayat M. The Effect of Glyphosate on Ovarian Tissue and in Vitro Maturation in Superovulated Mice. J Arak Uni Med Sci 2024; 27 (5) :271-278
URL: http://jams.arakmu.ac.ir/article-1-7817-en.html
1- Department of Anatomy, Students Research Committee, Arak University of Medical Sciences, Arak, Iran
2- Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
3- Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran , dr.mbayat@arakmu.ac.ir
Abstract:   (109 Views)
Introduction: Glyphosate is the most popular broad-spectrum herbicide globally due to the growing demand for glyphosate-resistant crops. Glyphosate exhibits harmful properties, including cytotoxicity, neurotoxicity, and reproductive toxicity. This study aimed to investigate the detrimental effects of glyphosate on ovarian histopathology in mice and the in vitro maturation (IVM) of oocytes following superovulation.
Methods: In this study, thirty-two female NMRI mice were randomly divided into the following groups: control, glyphosate, superovulation, and superovulation-glyphosate. Animals received glyphosate (0.5%) continuously through drinking water for three weeks. HMG and HCG were used to induce superovulation. Oocytes were collected from the ampulla, and the quantity and quality of oocytes were analyzed. Then, in vitro maturation (IVM) of oocytes was performed. At the end of the study, ovarian histopathology was analyzed.
Results: Compared to the control group, the glyphosate-treated group exhibited a significant decrease in secondary and Graafian follicles while demonstrating a concomitant increase in atretic follicles (P < 0.05). Additionally, the superovulation-glyphosate group showed fewer germinal vesicle breakdown (GVBD) and MII oocytes than the superovulation group. In the superovulation-glyphosate group, there was a notable reduction in GVBD and MII oocytes following in vitro maturation (IVM).
Conclusions: Glyphosate has the potential to damage ovarian tissue and adversely affect IVM and oogenesis.
Full-Text [PDF 1310 kb]   (105 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2024/09/18 | Accepted: 2024/11/20

References
1. Zhang J-W, Xu D-Q, Feng X-Z. The toxic effects and possible mechanisms of glyphosate on mouse oocytes. Chemosphere. 2019;237:124435. pmid: 31352102 doi: 10.1016/j.chemosphere.2019.124435
2. Duke SO, Powles SB. Glyphosate: a once‐in‐a‐century herbicide. Pest Manag Sci. 2008;64(4):319-25. pmid: 18273882 doi: 10.1002/ps.1518
3. Schimpf MG, Milesi MM, Ingaramo PI, Luque EH, Varayoud J. Neonatal exposure to a glyphosate based herbicide alters the development of the rat uterus. Toxicology. 2017;376:2-14. pmid: 27287056 doi: 10.1016/j.tox.2016.06.004
4. Bradberry SM, Proudfoot AT, Vale JA. Glyphosate poisoning. Toxicol Rev. 2004;23(3):159-67. pmid: 15862083 doi: 10.2165/00139709-200423030-00003
5. Serra L, Estienne A, Vasseur C, Froment P, Dupont J. Mechanisms of glyphosate and glyphosate-based herbicides action in female and male fertility in humans and animal models. Cells. 2021;10(11):3079. pmid: 34831302 doi: 10.3390/cells10113079
6. Spinaci M, Nerozzi C, Tamanini Cl, Bucci D, Galeati G. Glyphosate and its formulation Roundup impair pig oocyte maturation. Sci Rep. 2020;10(1):12007. doi: 10.1038/s41598-020-68813-6
7. Romano RM, Romano MA, Bernardi MM, Furtado PV, Oliveira CA. Prepubertal exposure to commercial formulation of the herbicide glyphosate alters testosterone levels and testicular morphology. Arch Toxicol. 2010;84(4):309-17. pmid: 20012598 doi: 10.1007/s00204-009-0494-z
8. Hamdaoui L, Naifar M, Rahmouni F, Harrabi B, Ayadi F, Sahnoun Z, et al. Subchronic exposure to kalach 360 SL-induced endocrine disruption and ovary. damage in female rats. Arch Physiol Biochem. 2018;124(1):27-34. pmid: 28708416 doi: 10.1080/13813455.2017.1352606
9. Ren X, Li R, Liu J, Huang K, Wu S, Li Y, et al. Effects of glyphosate on the ovarian function of pregnant mice, the secretion of hormones and the sex ratio of their fetuses. Environ Pollut. 2018; 243(Pt B):833-41. pmid: 30245445 doi: 10.1016/j.envpol.2018.09.049
10. Ingaramo PI, Schimpf MG, Milesi MM, Luque EH, Varayoud J. Acute uterine effects and long-term reproductive alterations in postnatally exposed female rats to a mixture of commercial formulations of endosulfan and glyphosate. Food Chem Toxicol. 2019;134:110832. pmid: 31550491 doi: 10.1016/j.fct.2019.110832
11. Ganesan S, Keating AF. Ovarian mitochondrial and oxidative stress proteins are altered by glyphosate exposure in mice. Toxicol Appl Pharmacol. 2020;402:115116. pmid: 32634520 doi: 10.1016/j.taap.2020.115116
12. Yahfoufi ZA, Bai D, Khan SN, Chatzicharalampous C, Kohan-Ghadr H-R, Morris RT, et al. Glyphosate induces metaphase II oocyte deterioration and embryo damage by zinc depletion and overproduction of reactive oxygen species. Toxicology. 2020;439:152466. pmid: 32315717 doi: 10.1016/j.tox.2020.152466
13. Williams CJ, Erickson GF, Feingold KR, Anawalt B, Blackman MR, Boyce A, et al. Morphology and Physiology of the Ovary. South Dartmouth (MA): MDText.com, Inc.; 2000. pmid: 25905186
14. Linher K, Dyce P, Li J. Characterization of primordial germ cell-like cells derived from porcine skin stem cells PLoS One.
15. 2009;4(12):e8263. pmid: 20011593 doi: 10.1371/journal.pone.0008263
16. Mahmoud AA, Elfiky AM, Abo-Zeid FS. The anti-androgenic effect of quercetin on hyperandrogenism and ovarian dysfunction induced in a dehydroepiandrosterone rat model of polycystic ovary syndrome. Steroids. 2022;177:108936. pmid: 34752810 doi: 10.1016/j.steroids.2021.108936
17. Lewis NB. New developments in female contraception: an immunocontraceptive vaginal film.[Thesis]. Boston, MA: Boston University; 2024.
18. Williams AL, Watson RE, DeSesso JM. Developmental and reproductive outcomes in humans and animals after glyphosate exposure: a critical analysis. J Toxicol Environ Health B Crit Rev. 2012;15(1):39-96. pmid: 22202229 doi: 10.1080/10937404.2012.632361
19. Milesi MM, Lorenz V, Durando M, Rossetti MF, Varayoud J. Glyphosate herbicide: reproductive outcomes and multigenerational effects. Front Endocrinol (Lausanne). 2021;12:672532. pmid: 34305812 doi: 10.3389/fendo.2021.672532
20. Mohammadi K, Sani MA, Safaei P, Rahmani J, Molaee-Aghaee E, Jafari SM. A systematic review and meta-analysis of the impacts of glyphosate on the reproductive hormones. Environ Sci Pollut Res Int. 2022;29(41):62030-41. pmid: 34453247 doi: 10.1007/s11356-021-16145-x
21. Parvez S, Gerona R, Proctor C, Friesen M, Ashby J, Reiter J, et al. Glyphosate exposure in pregnancy and shortened gestational length: a prospective Indiana birth cohort study. Environ Health. 2018;17(1):23. pmid: 29519238 doi: 10.1186/s12940-018-0367-0
22. Milesi MM, Lorenz V, Pacini G, Repetti MR, Demonte LD, Varayoud J, et al. Perinatal exposure to a glyphosate-based herbicide impairs female reproductive outcomes and induces second-generation adverse effects in Wistar rats. Arch Toxicol. 2018;92(8):2629-43. pmid: 29947892 doi: 10.1007/s00204-018-2236-6
23. Kaboli Kafshgiri S, Farkhondeh T, Miri-Moghaddam E. Glyphosate effects on the female reproductive systems: a systematic review. Rev Environ Health. 2022;37(4):487-500. pmid: 34265884 doi: 10.1515/reveh-2021-0029
24. Ingaramo P, Alarcón R, Munoz-de-Toro M, Luque EH. Are glyphosate and glyphosate-based herbicides endocrine disruptors that alter female fertility? Mol Cell Endocrinol. 2020;518:110934. pmid: 32659439 doi: 10.1016/j.mce.2020.110934
25. Shafiee Mehr M, Haeri SMJ, Barzroodi Pour M, Bayat M. Vitamin E improves oxidative stress, apoptosis, and steroidogenesis impairment in glyphosate-induced mice. Drug Chem Toxicol. 2024:1-9. pmid: 39478355 doi: 10.1080/01480545.2024.2417954
26. Hamdaoui L, Oudadesse H, Lefeuvre B, Mahmoud A, Naifer M, Badraoui R, et al. Sub-chronic exposure to Kalach 360 SL, Glyphosate-based Herbicide, induced bone rarefaction in female Wistar rats. Toxicology. 2020;436:152412. pmid: 32145347 doi: 10.1016/j.tox.2020.152412
27. Zhiqiang E, Zhao Y, Sun J, Zhang X, Jin Q, Gao Q. Glyphosate decreases bovine oocyte quality by inducing oxidative stress and apoptosis. Zygote. 2022;30(5):704-11. pmid: 35677960 doi: 10.1017/S0967199422000181

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